LOCUS       sequence-46159-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of CaMKIIa-driven hChR2 variant for ultrafast 
            optogenetic control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R,
            Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
  TITLE     Principles for applying optogenetic tools derived from direct 
            comparative analysis of microbial opsins.
  JOURNAL   Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
  PUBMED    22179551
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-18- 18"; volume: "9"; 
            issue: "2"; pages: "159-72"
COMMENT     Sequence Label: pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      226..235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat
       61 gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct
      121 cgtcagtcaa gccggttctc cgtttgcact caggagcacg ggcaggcgag tggcccctag
      181 ttctgggggc agctctagag cggtaccgga tccccgggta ccggtgccac catggactat
      241 ggcggcgctt tgtctgccgt cggacgcgaa cttttgttcg ttactaatcc tgtggtggtg
      301 aacgggtccg tcctggtccc tgaggatcaa tgttactgtg ccggatggat tgaatctcgc
      361 ggcacgaacg gcgctcagac cgcgtcaaat gtcctgcagt ggcttgcagc aggattcagc
      421 attttgctgc tgatgttcta tgcctaccaa acctggaaat ctacatgcgg ctgggaggag
      481 atctatgtgt gcgccattga aatggttaag gtgattctcg agttcttttt tgagtttaag
      541 aatccctcta tgctctacct tgccacagga caccgggtgc agtggctgcg ctatgcaacc
      601 tggctgctca cttgtcctgt catccttatc cacctgagca acctcaccgg cctgagcaac
      661 gactacagca ggagaaccat gggactcctt gtctcagaca tcgggtgtat cgtgtggggg
      721 gctaccagcg ccatggcaac cggctatgtt aaagtcatct tcttttgtct tggattgtgc
      781 tatggcgcga acacattttt tcacgccgcc aaagcatata tc
//
