LOCUS       sequence-461629-        6472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Cosma CL, Klein K, Kim R, Beery D, Ramakrishnan L
  TITLE     Mycobacterium marinum Erp is a virulence determinant required for 
            cell wall integrity and intracellular survival.
  JOURNAL   Infect Immun. 2006 Jun . 74(6):3125-33.
  PUBMED    16714540
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun. 2006 Jun . 74(6):3125-33."
COMMENT     Sequence Label: pGFPHYG2
FEATURES             Location/Qualifiers
     source          1..6472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(129..148)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(213..232)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2322..2910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2811..2830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4961..4977
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4961..4977
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5542..5558)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5543..5559)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             5613..6326
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5643..5671)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6266..6286
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      6404..6450
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctagccacc tgacgtcggg ggggggggga aagccacgtt gtgtctcaaa atctctgatg
       61 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      121 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg
      181 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      241 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc catgcgccag agttgtttct
      301 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      361 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgacta
      421 gagggggcgt caggcgccgg gggcggtgtc cggcggcccc cagaggaact gcgccagttc
      481 ctccggatcg gtgaagccgg agagatccag cggggtctcc tcgaacacct cgaagtcgtg
      541 caggaaggtg aaggcgagca gttcgcgggc gaagtcctcg gtccgcttcc actgcgcccc
      601 gtcgagcagc gcggccagga tctcgcggtc gccccggaag gcgttgagat gcagttgcac
      661 caggctgtag cgggagtctc ccgcatagac gtcggtgaag tcgacgatcc cggtgacctc
      721 ggtcgcggcc aggtccacga agatgttggt cccgtgcagg tcgccgtgga cgaaccgggg
      781 ttcgcggccg gccagcagcg tgtccacgtc cggcagccag tcctccaggc ggtccagcag
      841 ccggggcgag aggtagcccc acccgcggtg gtcctcgacg gtcgccgcgc ggcgttcccg
      901 cagcagttcc gggaagacct cggaatgggg ggtgagcacg gtgttcccgg tcagcggcac
      961 cctgtgcagc cggccgagca cccggccgag ttcgcgggcc agggcgagca gcgcgttccg
     1021 gtcggtcgtg ccgtccatcg cggaccgcca ggtggtgccg gtcatccggc tcatcaccag
     1081 gtagggccac ggccaggctc cggtgccggg ccgcagctcg ccgcggccga ggaggcgggg
     1141 caccggcacc ggggcgtccg ccaggaccgc gtacgcctcc gactccgacg cgaggctctc
     1201 cggaccgcac cagtgctcgc cgaacagctt gatcaccggg ccgggctcgc cgaccagtac
     1261 ggggttggtg ctctcgccgg gcacccgcag caccggcggc accggcagcc cgagctcctc
     1321 cagggctcgg cgggccagcg gctcccagaa ttcctggtcg ttccgcaggc tcgcgtagga
     1381 atcatccgaa tcaatacggt cgagaagtaa cagggattct tgtgtcacag cggacctcta
     1441 ttcacagggt acgggccggc ttaattccgc acggccggtc gcgacacggc ctgtccgcac
     1501 cgcggatcag gcgttgacga tgacgggctg gtcggccacg tcggggagtc aggcaactat
     1561 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1621 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1681 aaggatctag gtgaagatcc tttttgataa tctcatgctc tagtaatctt ttgccattct
     1741 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     1801 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     1861 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     1921 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     1981 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     2041 gacgggacgg cggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac
     2101 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg
     2161 gtccacctac aacaaagctc tcaccaaccg tggctccctc actttctggc tggatgatgg
     2221 ggcgattcag gcctggtatg agtcagcaac accttcttca cgaggcagac ctcactagtt
     2281 ccatgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2461 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2701 ctacagcgtg agctatgaga aagcgccacg cttcccgagg ggagaaaggc ggacaggtat
     2761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2941 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3001 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3061 cgcaacgcgt gagcccacca gctccgtaag ttcgggcgct gtgtggctcg tacccgcgca
     3121 ttcaggcggc agggggtcta acgggtctaa ggcggcgtgt acggccgcca cagcggctct
     3181 cagcggcccg gaaacgtcct cgaaacgacg catgtgttcc tcctggttgg tacaggtggt
     3241 tgggggtgct cggctgtcgc tggtgttcca ccaccagggc tcgacgggag agcgggggag
     3301 tgtgcagttg tggggtggcc cctcagcgaa atatctgact tggagctcgt gtcggaccat
     3361 acaccggtga ttaatcgtgg tctactacca agcgtgagcc acgtcgccga cgaatttgag
     3421 cagctctggc tgccgtactg gccgctggca agcgacgatc tgctcgaggg gatctaccgc
     3481 caaagccgcg cgtcggccct aggccgccgg tacatcgagg cgaacccaac agcgctggca
     3541 aacctgctgg tcgtggacgt agaccatcca gacgcagcgc tccgagcgct cagcgcccgg
     3601 gggtcccatc cgctgcccaa cgcgatcgtg ggcaatcgcg ccaacggcca cgcacacgca
     3661 gtgtgggcac tcaacgcccc tgttccacgc accgaatacg cgcggcgtaa gccgctcgca
     3721 tacatggcgg cgtgcgccga aggccttcgg cgcgccgtcg atggcgaccg cagttactca
     3781 ggcctcatga ccaaaaaccc cggccacatc gcctgggaaa cggaatggct ccactcagat
     3841 ctctacacac tcagccacat cgaggccgag ctcggcgcga acatgccacc gccgcgctgg
     3901 cgtcagcaga ccacgtacaa agcggctccg acgccgctag ggcggaattg cgcactgttc
     3961 gattccgtca ggttgtgggc ctatcgtccc gccctcatgc ggatctacct gccgacccgg
     4021 aacgtggacg gactcggccg cgcgatctat gccgagtgcc acgcgcgaaa cgccgaattt
     4081 ccgtgcaacg acgtgtgtcc cggaccgcta ccggacagcg aggtccgcgc catcgccaac
     4141 agcatttggc gttggatcac aaccaagtcg cgcatttggg cggacgggat cgtggtctac
     4201 gaggccacac tcagtgcgcg ccagtcggcc atctcgcgga agggcgcagc agcgcgcacg
     4261 gcggcgagca cagttgcgcg gcgcgcaaag tccgcgtcag ccatggaggc attgctatga
     4321 gcgacggcta cagcgacggc tacaaccggc agccgactgt ccgcaaaaag cggcgcgtga
     4381 ccgccgccga aggcgctcga atcaccggac tatccgaacg ccacgtcgtc cggctcgtgg
     4441 cgcaggaacg cagcgagtgg ctcgccgagc aggctgcacg ccgcgaacgc atccgcgcct
     4501 atcacgacga cgagggccac tcttggccgc aaacggccaa acatttcggg ctgcatctgg
     4561 acaccgttaa gcgactcggc tatcgggcga ggaaagagcg tgcggcagaa caggaagcgg
     4621 ctcaaaaggc ccacaacgaa gccgacaatc caccgctgtt ctaacgcaat tggggagcgg
     4681 gtgtcgcggg ggttccgtgg ggggttccgt tgcaacgggt cggacaggta aaagtcctgg
     4741 tagacgctag ttttctggtt tgggccatgc ctgtctcgtt gcgtgtttcg ttgcgcccgt
     4801 tttgaatacc agccagacga gacggggttc tacgaatctt ggtcgatacc aagccatttc
     4861 cgctgaatat cggggagctc accgccagaa tcggtggttg tggtgatgta cgtggcgaac
     4921 tccgttgtag tgcctgtggt ggcatccgtg gcgcggccgc tctagaacta gtggatctga
     4981 cccgctccac aaccgctgag cagggtggca gtcagagcga ccaggccgac cgatacgagg
     5041 cggattttga acaggttcac tcgaagcctc ccgctgggtt cggagcatcg tcgatcacat
     5101 gacggcgtgc tgacaccgat tcgactatgc acagtagtag gtagccgccg cggacggtag
     5161 cgcaggcggc ctcggcgccg tcgtggcgtc gcgatggttg gcgccagacc cgcctgatcg
     5221 gcgtcccgac cagggccgcc ggggtggtga gcctcggctt ggtccagccg tagtgagacg
     5281 ggatgtcttc ccggttgtcg ggtttgggtg ggcggtcaca tgttcgctgg ctgtgggcgt
     5341 gtcgggggtc gtcgaagcgg ccgcgaacag ggctggacga tgcacgtctt gcacctcctg
     5401 tcaaccccca atgttgagcc gcgatgcccc tgacctgcac cgttggaccg cagtcgttag
     5461 gggggcgtgt tagcatggag tgacgaaagg ggcctaaatc agatgatccc ccgggctgca
     5521 ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg gtaccagatc
     5581 tttaaatcta gatttaagaa ggagatatac atatgagtaa aggagaagaa cttttcactg
     5641 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     5701 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     5761 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
     5821 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
     5881 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
     5941 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
     6001 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
     6061 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
     6121 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
     6181 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
     6241 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     6301 cacatggcat ggatgaacta tacaaataaa tgtccagacc tgcaggcatg caagcttatc
     6361 gatgtcgacg tagttaacta gcgtacgatc gactgccagg catcaaataa aacgaaaggc
     6421 tcagtcgaaa gactgggcct ttcgttttat gccatcatgg ccgcggtgat ca
//
