LOCUS       sequence-461664-        4204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry red fluorescent protein (RFP) reporter protein with an amber
            stop codon at position 13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdelkader EH, Qianzhu H, Otting G, Huber T
  TITLE     Biosynthesis and genetic encoding of activated nitriles for fast 
            protein conjugation and tunable fluorogenic labeling
  JOURNAL   Chem 102385
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            102385"
COMMENT     Sequence Label: pCDF-RFP amber
FEATURES             Location/Qualifiers
     source          1..4204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             55..77
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..120
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             124..798
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(885..903)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      899..946
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1124..1915)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1916..2007)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2055..2793)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(3003..4085)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4047..4066
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4086..4163)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 aaattaatac gactcactat agggagacca caacggtttc cctctagaaa taattttgtt
       61 taactttaag aaggagatat acatatggct tctatgaccg gtcatcacca tcaccatcac
      121 taggccagta gtgaagacgt tatcaaggag tttatgcgtt tcaaagtacg tatggagggt
      181 agtgttaacg gacacgaatt tgagatcgag ggagaggggg aaggtcgtcc ttacgaggga
      241 actcaaacgg ccaaattaaa ggtgaccaaa ggtgggccct tgccattcgc gtgggacatc
      301 ttgtcacccc agttccagta cgggtcgaag gcatacgtaa aacacccagc ggacattcct
      361 gactatctta agttatcttt cccggaaggt tttaaatggg aacgcgtgat gaactttgag
      421 gatggggggg ttgttacggt gacacaagac tcctcattgc aagatggaga gtttatctat
      481 aaagtcaaac ttcgcggcac caattttcca tctgacggtc ctgtaatgca gaaaaaaaca
      541 atgggctggg aagcctccac agaacgtatg taccccgaag atggagcttt aaagggcgaa
      601 attaaaatgc gcttaaaact taaagacggc ggccattacg acgccgaagt gaaaacgacg
      661 tatatggcta agaaacccgt ccagcttccg ggagcctata aaactgacat caaactggat
      721 attacatcac acaacgaaga ttatactatt gtcgaacagt acgaacgcgc cgaaggccgc
      781 cattcaacgg gagcataaga attcgagctc ccgggtacca tggcatgcat cgatagatcc
      841 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      901 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      961 tacaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc
     1021 agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc gggtcatcgt
     1081 ggccggatct tgcggcccct cggcttgaac gaattgttag acattatttg ccgactacct
     1141 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
     1201 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
     1261 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     1321 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     1381 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     1441 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     1501 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     1561 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     1621 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     1681 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     1741 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     1801 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     1861 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatactct
     1921 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1981 ttgaatgtat ttagaaaaat aaacaaatag ctagctcact cggtcgctac gctccgggcg
     2041 tgagactgcg gcgggcgctg cggacacata caaagttacc cacagattcc gtggataagc
     2101 aggggactaa catgtgaggc aaaacagcag ggccgcgccg gtggcgtttt tccataggct
     2161 ccgccctcct gccagagttc acataaacag acgcttttcc ggtgcatctg tgggagccgt
     2221 gaggctcaac catgaatctg acagtacggg cgaaacccga caggacttaa agatccccac
     2281 cgtttccggc gggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg tttaccggat
     2341 acctgttccg cctttctccc ttacgggaag tgtggcgctt tctcatagct cacacactgg
     2401 tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga actccccgtt
     2461 cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc ggaaaagcac
     2521 ggtaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc
     2581 taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga aggacagatt
     2641 tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca actgacttaa
     2701 ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc
     2761 agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgct ctagatttca
     2821 gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa
     2881 ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
     2941 agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc
     3001 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3061 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
     3121 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
     3181 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
     3241 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc
     3301 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
     3361 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
     3421 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
     3481 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
     3541 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
     3601 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
     3661 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
     3721 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
     3781 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
     3841 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
     3901 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
     3961 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
     4021 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
     4081 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
     4141 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat
     4201 tagg
//
