LOCUS       sequence-461679-       11019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one 
            grazoprevir-inducible expression of mouse neurogenin 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Blanch-Asensio A, Ploessl DS, Wang NB, Mummery CL, Galloway KE, 
            Davis RP
  TITLE     STRAIGHT-IN Dual: a platform for dual, single-copy integrations of 
            DNA payloads and gene circuits into human induced pluripotent stem 
            cells.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 
            10.1101/2024.10.17.616637.
  PUBMED    39464154
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 
            10.1101/2024.10.17.616637."
COMMENT     Sequence Label: Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor
FEATURES             Location/Qualifiers
     source          1..11019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        275..471
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      322..457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        558..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          789..1727
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1684..1704
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1744..1753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1756..1793
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    1808..1855
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             3187..3213
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3223..3249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3259..3285
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3296..3884
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3349..3369)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3767..3778)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     enhancer        3941..4320
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4596..5612
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5536..5558
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5620..5639
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6230..6295
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6302..6322
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7172..7198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7424..7795
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     polyA_signal    7805..8029
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8077..8110
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(8118..8134)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8148..8164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8157..8179)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8377..8832
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8464..8483)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8674..8695
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8849..8868)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8968..8990
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9028..9046)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9114..9218
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9219..9287,9288..10079)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9437..9456)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10250..10838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10739..10758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10992..11009
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggactccg tcgtcaggat catccggatc
     1801 cggatccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcatgat
     1861 attcggcaag caggcatcga ctagttaatt aagcggacga ttgagtaaca ctgtatctag
     1921 tctacctaca gggctcggcg tagccgatgt cgcgcggata gcgtcaacct cttcggcgta
     1981 gccgatgtcg cgcaatcagt tctcccgagg tcggcgtagc cgatgtcgcg cagagccgtg
     2041 cttccaatac ggcgtagccg atgtcgcgcc gggtttcgta acaatcgcat gaggattcgc
     2101 aacgccttcg gcgtagccga tgtcgcgctc ccgtctcagt aaaggtcggc gtagccgatg
     2161 tcgcgcaatc ggactgcctt cgtacggcgt agccgatgtc gcgcgtatca gtcgcctcgg
     2221 aacggcgtag ccgatgtcgc gcattcgtaa gaggctcact ctcccttaca cggagtggat
     2281 aggatcctct agagggtata taatgggggc cactagtcta ctaccagagc tcatcgctag
     2341 cgctaccgga tcgccaccat gttcgtcaaa tctgagactc tggagttgaa ggaggaagag
     2401 gaggtactga tgctgctggg ctcggcttcc ccggcctcgg cgaccctgac cccgatgtcc
     2461 tccagcgcgg acgaggagga ggacgaggag ctgcgccggc cgggctccgc gcgtgggcag
     2521 cgtggagcgg aagccgggca gggggtgcag ggcagtccgg cgtcgggtgc cgggggttgc
     2581 cggccagggc ggctgctggg cctgatgcac gagtgcaagc gtcgcccgtc gcgctcacgg
     2641 gccgtctccc gaggtgccaa gacggcggag acggtgcagc gcatcaagaa gacccgcagg
     2701 ctcaaggcca acaaccgcga gcgcaaccgc atgcacaacc taaacgccgc gctggacgcg
     2761 ctgcgcgagg tgctgcccac cttccccgag gatgccaagc tcacgaagat cgagacgctg
     2821 cgcttcgccc acaattacat ctgggcgctc accgagactc tgcgcctggc ggaccactgc
     2881 gccggcgccg gtggcctcca gggggcgctc ttcacggagg cggtgctcct gagcccggga
     2941 gctgcgctcg gcgccagcgg ggacagccct tctccacctt cctcctggag ctgcaccaac
     3001 agcccggcgt catcctccaa ctccacgtcc ccatacagct gcactttatc gcccgctagc
     3061 cccgggtcag acgtggacta ctggcagccc ccacctccgg agaagcatcg ttatgcgcct
     3121 cacctgcccc tcgccaggga ctgtatcggg tctggctccg gatcaagtgg tggcagcgga
     3181 tcaggatatc catacgatgt accagactat gcgggatcgg gttaccccta cgacgtcccc
     3241 gactacgccg gctccggcta cccctacgac gtccctgatt atgcttagcg gccgcaatca
     3301 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3361 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3421 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3481 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3541 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3601 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3661 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     3721 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3781 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3841 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgccctttg ggccgcctcc
     3901 ccgcgctagt gctggggatg cggtgggctc tatgggtctc gacattgatt attgactagt
     3961 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4021 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4081 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4141 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4201 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4261 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4321 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     4381 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     4441 gggcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     4501 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     4561 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
     4621 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
     4681 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
     4741 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
     4801 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
     4861 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     4921 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     4981 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     5041 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     5101 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     5161 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     5221 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     5281 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     5341 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     5401 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     5461 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     5521 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     5581 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     5641 tgtctcatca ttttggcaaa gaattccgat cgcgccacct gccgccacca tgtctagacc
     5701 cggagagcgc ccattccagt gtcggatttg catgcggaac ttttcgagaa gacacggcct
     5761 ggacagacat acccgtactc atacaggtga aaaacccttt cagtgtcgga tctgtatgcg
     5821 aaatttctcc gaccacagca gcctgaagag acatctacgt acccacaccg gcagccagaa
     5881 gccatttcag tgtcggatct gtatgcggaa cttctccgtg agacacaacc tgaccagaca
     5941 tctacgtacg cacaccggag agaagccatt ccaatgccga atatgcatgc gcaacttcag
     6001 tgaccacagc aacctgagca gacacctaaa aacccacacc ggttcccaga agccatttca
     6061 gtgtcggatc tgtatgcgga acttctccca gcgcagcagc ctggtgagac atctacgtac
     6121 gcacaccgga gagaagccat tccaatgccg aatatgcatg cgcaacttca gtgagagcgg
     6181 ccacctgaag agacacctgc gtacgcacct gaggggatcc acctgcaggg actacaaaga
     6241 ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg acaagatggc
     6301 ccccaagaaa aagaggaagg tgggcattca cggggtgccg ggtggactcg agggaggcgg
     6361 tggaagcggc ggtaccgagg acgtggtgtg ctgccactca atctacggca agaagaaggg
     6421 tgatatcgac acctaccgat acataggctc ttccgggaca ggctgcgtgg tcatagtggg
     6481 caggatcgtc ttgtccggat ccggcactag tgcgcccatc acggcgtacg cccagcagac
     6541 gagaggcctc ctagggtgta taatcaccag cctgactggc cgggacaaaa accaagtgga
     6601 gggtgaggtc cagatcgtgt caactgctac ccaaaccttc ctggcaacgt gcatcaatgg
     6661 ggtatgctgg gcagtctacc acggggccgg aacgaggacc atcgcatcac ccaagggtcc
     6721 tgtcatccag atgtatacca atgtggacca agaccttgtg ggctggcccg ctcctcaagg
     6781 ttcccgctca ttgacaccct gtacctgcgg ctcctcggac ctttacctgg tcacgaggca
     6841 cgccgatgtc attcccgtgc gccggcgagg tgatagcagg ggtagcctgc tttcgccccg
     6901 gcccatttcc tacttgaaag gctcctcggg gggtccgctg ttgtgccccg cgggacacgc
     6961 cgtgggccta ttcagggccg cggtgtgcac ccgtggagtg gctaaagcgg tggactttat
     7021 ccctgtggag aacctagaga caaccatgag atccccggtg ttcacggaca actcctctcc
     7081 accagcagtc accctgacgc acccaatcac caaaatcgat agggaggttc tctaccagga
     7141 gttcgatgag atggaagagt gctctcagca ctatccctac gatgtgcccg attacgctgg
     7201 aggcggtgga agcggcggta ccgatgagtt tcccaccatg gtgtttcctt ctgggcagat
     7261 cagccaggcc tcggccttgg ccccggcccc tccccaagtc ctgccccagg ctccagcccc
     7321 tgcccctgct ccagccatgg tatcagctct ggcccaggcc ccagcccctg tcccagtcct
     7381 agccccaggc cctcctcagg ctgtggcccc acctgccccc aagcccaccc aggctgggga
     7441 aggaacgctg tcagaggccc tgctgcagct gcagtttgat gatgaagacc tgggggcctt
     7501 gcttggcaac agcacagacc cagctgtgtt cacagacctg gcatccgtcg acaactccga
     7561 gtttcagcag ctgctgaacc agggcatacc tgtggccccc cacacaactg agcccatgct
     7621 gatggagtac cctgaggcta taactcgcct agtgacaggg gcccagaggc cccccgaccc
     7681 agctcctgct ccactggggg ccccggggct ccccaatggc ctcctttcag gagatgaaga
     7741 cttctcctcc attgcggaca tggacttctc agccctgctg agtcagatca gctcccaatt
     7801 gtaactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7861 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7921 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     7981 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc tagccgctgc
     8041 aagaactctt cctcacggat ccgaagttcc tattccgaag ttcctattct tcaaatagta
     8101 taggaacttc agcgctcact ggccgtcgtt ttacaaaggg cgaattcact ggccgtcgtt
     8161 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     8221 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     8281 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     8341 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     8401 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     8461 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8521 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8581 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8641 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8701 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     8761 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     8821 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8881 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     8941 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     9001 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     9061 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     9121 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9181 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9241 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9301 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9361 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9421 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9481 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9541 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9601 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9661 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9721 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9781 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9841 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9901 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9961 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    10021 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    10081 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    10141 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    10201 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10261 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10321 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10381 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10441 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10501 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10561 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10621 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10681 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10741 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10801 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10861 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10921 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10981 cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
