LOCUS       sequence-46168-e        5204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5204)
  TITLE     pHyVec Cloning Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHyVec13
FEATURES             Location/Qualifiers
     source          1..5204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     428..447
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     661..683
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     675..692
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     676..692
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     702..721
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        702..720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1790..2506
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1820..1848)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2443..2463
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3016..3036)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3016..3034)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3055..3071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3055..3071)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3068..3090)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3079..3095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3103..3109,3110..3127,3128..3133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3148..3169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3286..3303)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3457..4045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3537..3556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4216..5007,5008..5076))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4839..4858
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5077..5181)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccggatcca aggtaccttc tcgagttact agtagtctag actatcgata ggatatctca
      361 gatctaggtc gacgagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
      421 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
      481 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
      541 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      601 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      661 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
      721 gcgaattggt tttaagccga gatgcaggat tctgaatgaa aaagaaaaaa agaagtctcg
      781 gtagagtaaa agtgatcggt ttgcaactgt aaaatttatt gaagtaccaa taattttatt
      841 taaaataaaa ctgaaatata aagttaaagt tgctgttcta taagtttacc gaattttaaa
      901 accattgtaa cgctagagta atatttgagt ctactaagtt agtccccgca ctttttaatc
      961 aagcaataaa tacccaaact ttgcttattc aaatcaataa accaatatat ctcttaaaat
     1021 aaagtaaaaa cttctgaaat tctataaaaa aaaatttaat ttcgaaatat caaatgtaac
     1081 ttcaacaccg cactattttc ttttaaacaa ctgatatagt aattacttct caaaaacgtt
     1141 atctcaaggt ttgtgatgta cttaaaacca ctcctatttt gttacgcgtt taaaaaagca
     1201 aacataagtt ggtttctatt gatgaatgag aacatatttc atttaaagtt aaaatcctac
     1261 cagtggtttc actgtacgta aacaccgtca aaaaaacagg aacgttttta aagattaata
     1321 attgaagtaa aaaaaattta ataccggggg ttaaaaaaat cttttaaaat aattataaat
     1381 atatatatta aaatttataa atttttaaac acatttaaaa tatatattaa gtataataaa
     1441 agtaatatta taaaaaaaaa tttaatttta taattatttt tattaaattt ataaataata
     1501 ggtaaaactt acatatccgt tttatttttt cttaataaaa taacgcgtgc aaatttttgt
     1561 ccatataaag accttttcga acaataactt ttttgcttag ccgttttttt tcttatatgg
     1621 tcaaaaaagc gctcaagcga ttcaccataa aaagcgcaat tagttcagcg ttcgttattc
     1681 agaagcttca gctttgcttg atactcagct cttctctttt taaacaaaac acttaatcaa
     1741 aatggccgat gatgaagttg ccgccctcgc tgcagccccg gtagaaaaaa tgagtaaagg
     1801 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
     1861 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
     1921 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
     1981 ctgttatggt gttcaatgct tttcaagata cccagatcat atgaaacggc atgacttttt
     2041 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     2101 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     2161 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     2221 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
     2281 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
     2341 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
     2401 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
     2461 aacagctgct gggattacac atggcatgga tgaactatac aaatagcatt cgtagaattc
     2521 acaattcgat tatatttata ctggactatt tttacatctg ttcggttatt ttcacattta
     2581 tttttctata tatatcttat aaacgtttta aaacccatgt aatttttgtt aagctgtaat
     2641 ataaaagacg tcctaacaaa cttcttttat tactgaattt cctttaatta taataaataa
     2701 caagttttaa aataaattca ggcaattaag gcgctcctga ggtactaaaa ttaatgtaaa
     2761 catttaaaat taacttggat ggtcttaagt actgtactcg tgattttgtt atactttatt
     2821 attagaaaag tcgtctatta actttttgtt ccttaattta cttgattaaa ttgtcgctta
     2881 atttatcaaa tcaggttttg cgcgttattt tagagaaaaa cttattagaa aaatgaataa
     2941 gcaaagttta ggctaacatg tttttttatt attttaaata gttcaagtca atgacgtata
     3001 aaccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     3061 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3121 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3181 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3241 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3301 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3361 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3421 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3481 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3541 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3601 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3661 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3721 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3781 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3841 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     3901 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3961 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4021 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4081 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4141 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4201 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4261 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4321 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4381 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4441 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4501 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4561 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4621 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4681 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4741 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4801 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4861 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4921 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4981 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5041 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5101 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5161 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
