LOCUS       sequence-461681-        9996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bxb1-GT donor plasmid with divergent syntax for constitutively 
            expressing mRuby2 and mGreenLantern by the respective promoters CAG 
            and hEF1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Blanch-Asensio A, Ploessl DS, Wang NB, Mummery CL, Galloway KE, 
            Davis RP
  TITLE     STRAIGHT-IN Dual: a platform for dual, single-copy integrations of 
            DNA payloads and gene circuits into human induced pluripotent stem 
            cells.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 
            10.1101/2024.10.17.616637.
  PUBMED    39464154
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 
            10.1101/2024.10.17.616637."
COMMENT     Sequence Label: Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent
FEATURES             Location/Qualifiers
     source          1..9996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(28..60)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(68..85)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(68..84)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(77..99)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      297..752
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(384..403)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     594..615
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(769..788)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     888..910
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(948..966)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1034..1138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1139..1207,1208..1999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1357..1376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2170..2758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2659..2678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2912..2929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3046..3067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3082..3087,3088..3105,3106..3112)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3120..3136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3125..3147
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3144..3160
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3144..3160
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3192..3402
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3214..3410
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3261..3396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3497..4675
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3728..4666
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      4683..4692
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4695..4732
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    complement(4741..4774)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4822..5046
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(5061..5771)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     regulatory      5768..5777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5803..5822)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5830..6846
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5884..5906)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6847..7124)
                     /label=chicken beta-actin promoter
     enhancer        7126..7429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(7665..7684)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     promoter        7794..8975
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8024..8966
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8977..9001
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      9031..9040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9037..9039,9040..9042,9043..9756)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(9082..9103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    9768..9992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgctcgctgc aagaactctt cctcacgata acttcgtata ggagacttta tacgaagtta
       61 agcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      121 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      181 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
      241 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
      301 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      361 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      421 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      481 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
      541 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      601 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
      661 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
      721 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
      781 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
      841 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
      901 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
      961 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1021 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1081 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1141 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1201 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1261 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1321 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1381 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1441 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1501 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1561 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1621 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1681 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1741 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1801 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1861 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1921 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1981 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2041 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2101 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2161 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2221 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2281 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2341 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2401 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2461 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2521 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2581 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2641 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2701 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2761 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2821 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2881 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2941 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     3001 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     3061 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     3121 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttaa
     3181 ttccctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3241 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3301 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3361 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttgca
     3421 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     3481 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     3541 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     3601 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     3661 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     3721 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     3781 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     3841 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     3901 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     3961 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     4021 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     4081 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     4141 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     4201 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     4261 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     4321 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     4381 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     4441 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     4501 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     4561 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     4621 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat
     4681 tcgccaccat ggccggcttg tcgacgacgg cggtctccgt cgtcaggatc atccggatcc
     4741 ataacttcgt atagcataca ttatacgaag ttatcatgat attcggcaag caggcatcga
     4801 ctagttaatt aagtactagc gccatagagc ccaccgcatc cccagcatgc ctgctattgt
     4861 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc
     4921 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac
     4981 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag
     5041 gcacaggtgc aacgttgtta cttgtacagc tcgtccatcc caccaccaag cccggcgaac
     5101 ttggcaactg cgtgttcgcg ttgtactacg aacatttcat tgtcactttc ctctaacctt
     5161 tccaggcggt gatcaacggc atggataccg ggcatcttga tgttcccgac ggtctttttt
     5221 gacctgtaag ttgttacgaa agagcaagac agatggccac caccatcaac tttcagtgcc
     5281 atatgagtgt atcccctcag accaccatct gctggataca tcatctctgt attaggctcc
     5341 caacccttgg tcttcttctg catcacggga ccattggagg gaaagtttac ccctctgact
     5401 tggacgtggt aaacgagaca gccatcctca aggctggtgt cctgcatgac ggtgacgact
     5461 ccaccatctt cgtatctcgt aactctttcc caagtaaaac cctcaggaaa ggactgttta
     5521 aagaaatcag gaatgccttt cgggtacttg ataaaagtac ggctgccata catgaacgac
     5581 gtggcaagaa tgtcaaaggc aaatggcagg ggtcctccct cgatgacttt gatcctcatg
     5641 gtttgagttc ccatgtacgg attgccttct ccttcacctg tgcatttgaa ttggtggccg
     5701 ttgaccgaac cttccatgac caccttcata cgcatatttt ccttgatcag ctcttcgccc
     5761 ttagacacca tggtggccat ttttgccaaa atgatgagac agcacaataa ccagcacgtt
     5821 gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg ttagcagagg ctctagagcc
     5881 gccggtcaca cgccagaagc cgaaccccgc cctgccccgt cccccccgaa ggcagccgtc
     5941 cccccgcgga cagccccgag gctggagagg gagaagggga cggcggcgcg gcgacgcacg
     6001 aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac cgcttcgccc cgcgcccgct
     6061 agagggggtg cggcggcgcc tcccagattt cggctccgca cagatttggg acaaaggaag
     6121 tccctgcgcc ctctcgcacg attaccataa aaggcaatgg ctgcggctcg ccgcgcctcg
     6181 acagccgccg gcgctccggg ggccgccgcg cccctccccc gagccctccc cggcccgagg
     6241 cggccccgcc ccgcccggca cccccacctg ccgccacccc ccgcccggca cggcgagccc
     6301 cgcgccacgc cccgtacgga gccccgcacc cgaagccggg ccgtgctcag caactcgggg
     6361 aggggggtgc agggggggtt gcagcccgac cgacgcgccc acaccccctg ctcacccccc
     6421 cacgcacaca ccccgcacgc agcctttgtt cccctcgcag ccccccccgc accgcggggc
     6481 accgcccccg gccgcgctcc cctcgcgcac actgcggagc gcacaaagcc ccgcgccgcg
     6541 cccgcagcgc tcacagccgc cgggcagcgc ggagccgcac gcggcgctcc ccacgcacac
     6601 acacacgcac gcaccccccg agccgctccc cccgcacaaa gggccctccc ggagcccctc
     6661 aaggctttca cgcagccaca gaaaagaaac aagccgtcat taaaccaagc gctaattaca
     6721 gcccggagga gaagggccgt cccgcccgct cacctgtggg agtaacgcgg tcagtcagag
     6781 ccggggcggg cggcgcgagg cggcggcgga gcggggcacg gggcgaaggc agcgcgcagc
     6841 gactcccgcc cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc gcctcgccat
     6901 aaaaggaaac tttcggagcg cgccgctctg attggctgcc gccgcacctc tccgcctcgc
     6961 cccgccccgc ccctcgcccc gccccgcccc gcctggcgcg cgcccccccc ccccccccgc
     7021 ccccatcgct gcacaaaata attaaaaaat aaataaatac aaaattgggg gtggggaggg
     7081 gggggagatg gggagagtga agcagaacgt ggggctcacc tcgaccatgg taatagcgat
     7141 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     7201 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     7261 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     7321 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     7381 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     7441 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tagtacgaac
     7501 atggtagcta gtggatcctt cgtcgacctg cagactggct gtgtataagg gagcctgaca
     7561 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
     7621 gatcagccac ttcttccccg ataacggaga ccggcacact ggcgcatatc ggtggtcatc
     7681 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacgcat atgaagctgg
     7741 acatcacctc ccacaacctc gaggtggaac agtacgctga tatctcttat actgctccgg
     7801 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     7861 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     7921 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     7981 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     8041 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     8101 gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     8161 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg
     8221 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     8281 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     8341 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     8401 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     8461 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     8521 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     8581 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     8641 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     8701 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     8761 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     8821 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     8881 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     8941 ttcaaagttt ttttcttcca tttcaggtgt cgtgagagct cgtttagtga accgtcagat
     9001 cgcctggaga cgccatccac gctgtaatga gccaccatgg tgagcaaggg cgaggagctg
     9061 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     9121 agcgtccgcg gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
     9181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt aggctacggc
     9241 gtggcctgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     9301 atgcccgaag gctacgtcca ggagcgcacc atctctttca aggacgacgg tacctacaag
     9361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcgt gctgaagggc
     9421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc
     9481 cacaaggtct atatcacggc cgacaagcag aagaacggca tcaaggctaa cttcaagacc
     9541 cgccacaacg ttgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     9601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagccatca gtccaaactg
     9661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctga aggagagggt gaccgccgcc
     9721 gggattacac atgacatgga cgagctgtac aagtaacaac gttgcacctg tgccttctag
     9781 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     9841 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     9901 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     9961 caggcatgct ggggatgcgg tgggctctat ggcgct
//
