LOCUS       sequence-461683-       10592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one 
            grazoprevir-inducible expression (empty) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Blanch-Asensio A, Ploessl DS, Wang NB, Mummery CL, Galloway KE, 
            Davis RP
  TITLE     STRAIGHT-IN Dual: a platform for dual, single-copy integrations of 
            DNA payloads and gene circuits into human induced pluripotent stem 
            cells.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 
            10.1101/2024.10.17.616637.
  PUBMED    39464154
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 
            10.1101/2024.10.17.616637."
COMMENT     Sequence Label: Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor
FEATURES             Location/Qualifiers
     source          1..10592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        275..471
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      322..457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        558..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          789..1727
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1684..1704
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1744..1753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1756..1793
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    1808..1855
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      2353..2362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2357..2366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2370..2391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2406..2411,2412..2429,2430..2436)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2444..2460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2468..2484
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2480..2803
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    2497..2553
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2554..2570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2869..3457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2922..2942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3340..3351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     enhancer        3514..3893
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3895..4168
                     /label=chicken beta-actin promoter
     intron          4169..5185
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5109..5131
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5193..5212
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5803..5868
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5875..5895
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6745..6771
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6997..7368
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     polyA_signal    7378..7602
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7650..7683
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(7691..7707)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7721..7737)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7950..8405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8037..8056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8247..8268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8541..8563
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8601..8619)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8687..8791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8792..8860,8861..9652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9010..9029)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9823..10411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10312..10331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10565..10582
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggactccg tcgtcaggat catccggatc
     1801 cggatccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcatgat
     1861 attcggcaag caggcatcga ctagttaatt aagcggacga ttgagtaaca ctgtatctag
     1921 tctacctaca gggctcggcg tagccgatgt cgcgcggata gcgtcaacct cttcggcgta
     1981 gccgatgtcg cgcaatcagt tctcccgagg tcggcgtagc cgatgtcgcg cagagccgtg
     2041 cttccaatac ggcgtagccg atgtcgcgcc gggtttcgta acaatcgcat gaggattcgc
     2101 aacgccttcg gcgtagccga tgtcgcgctc ccgtctcagt aaaggtcggc gtagccgatg
     2161 tcgcgcaatc ggactgcctt cgtacggcgt agccgatgtc gcgcgtatca gtcgcctcgg
     2221 aacggcgtag ccgatgtcgc gcattcgtaa gaggctcact ctcccttaca cggagtggat
     2281 aggatcctct agagggtata taatgggggc cactagtcta ctaccagagc tcatcgctag
     2341 cgctaccgga tcgccaccat ggcggccgct aatgtgagtt agctcactca ttaggcaccc
     2401 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     2461 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac
     2521 tctagaggat ccccgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga
     2581 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     2641 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     2701 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2761 catatggtgc actctcagta caatctgctc tgatgccgca tagcccagcc cgcctaatga
     2821 gcgggctttt ttttgaacaa attaagccta taacgcggaa gcggccgcaa tcaacctctg
     2881 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2941 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3001 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3061 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3121 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3181 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3241 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     3301 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3361 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3421 cagacgagtc ggatctccct ttgggccgcc tccccgccct ttgggccgcc tccccgcgct
     3481 agtgctgggg atgcggtggg ctctatgggt ctcgacattg attattgact agttattaat
     3541 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     3601 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     3661 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     3721 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     3781 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     3841 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     3901 tgagccccac gttctgcttc actctcccca tctccccccc ctcccccccc ccaattttgt
     3961 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggcgcgcgcc
     4021 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     4081 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     4141 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     4201 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     4261 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     4321 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     4381 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     4441 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     4501 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     4561 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     4621 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     4681 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     4741 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     4801 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     4861 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     4921 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     4981 agccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     5041 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     5101 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     5161 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     5221 tcattttggc aaagaattcc gatcgcgcca cctgccgcca ccatgtctag acccggagag
     5281 cgcccattcc agtgtcggat ttgcatgcgg aacttttcga gaagacacgg cctggacaga
     5341 catacccgta ctcatacagg tgaaaaaccc tttcagtgtc ggatctgtat gcgaaatttc
     5401 tccgaccaca gcagcctgaa gagacatcta cgtacccaca ccggcagcca gaagccattt
     5461 cagtgtcgga tctgtatgcg gaacttctcc gtgagacaca acctgaccag acatctacgt
     5521 acgcacaccg gagagaagcc attccaatgc cgaatatgca tgcgcaactt cagtgaccac
     5581 agcaacctga gcagacacct aaaaacccac accggttccc agaagccatt tcagtgtcgg
     5641 atctgtatgc ggaacttctc ccagcgcagc agcctggtga gacatctacg tacgcacacc
     5701 ggagagaagc cattccaatg ccgaatatgc atgcgcaact tcagtgagag cggccacctg
     5761 aagagacacc tgcgtacgca cctgagggga tccacctgca gggactacaa agaccatgac
     5821 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag
     5881 aaaaagagga aggtgggcat tcacggggtg ccgggtggac tcgagggagg cggtggaagc
     5941 ggcggtaccg aggacgtggt gtgctgccac tcaatctacg gcaagaagaa gggtgatatc
     6001 gacacctacc gatacatagg ctcttccggg acaggctgcg tggtcatagt gggcaggatc
     6061 gtcttgtccg gatccggcac tagtgcgccc atcacggcgt acgcccagca gacgagaggc
     6121 ctcctagggt gtataatcac cagcctgact ggccgggaca aaaaccaagt ggagggtgag
     6181 gtccagatcg tgtcaactgc tacccaaacc ttcctggcaa cgtgcatcaa tggggtatgc
     6241 tgggcagtct accacggggc cggaacgagg accatcgcat cacccaaggg tcctgtcatc
     6301 cagatgtata ccaatgtgga ccaagacctt gtgggctggc ccgctcctca aggttcccgc
     6361 tcattgacac cctgtacctg cggctcctcg gacctttacc tggtcacgag gcacgccgat
     6421 gtcattcccg tgcgccggcg aggtgatagc aggggtagcc tgctttcgcc ccggcccatt
     6481 tcctacttga aaggctcctc ggggggtccg ctgttgtgcc ccgcgggaca cgccgtgggc
     6541 ctattcaggg ccgcggtgtg cacccgtgga gtggctaaag cggtggactt tatccctgtg
     6601 gagaacctag agacaaccat gagatccccg gtgttcacgg acaactcctc tccaccagca
     6661 gtcaccctga cgcacccaat caccaaaatc gatagggagg ttctctacca ggagttcgat
     6721 gagatggaag agtgctctca gcactatccc tacgatgtgc ccgattacgc tggaggcggt
     6781 ggaagcggcg gtaccgatga gtttcccacc atggtgtttc cttctgggca gatcagccag
     6841 gcctcggcct tggccccggc ccctccccaa gtcctgcccc aggctccagc ccctgcccct
     6901 gctccagcca tggtatcagc tctggcccag gccccagccc ctgtcccagt cctagcccca
     6961 ggccctcctc aggctgtggc cccacctgcc cccaagccca cccaggctgg ggaaggaacg
     7021 ctgtcagagg ccctgctgca gctgcagttt gatgatgaag acctgggggc cttgcttggc
     7081 aacagcacag acccagctgt gttcacagac ctggcatccg tcgacaactc cgagtttcag
     7141 cagctgctga accagggcat acctgtggcc ccccacacaa ctgagcccat gctgatggag
     7201 taccctgagg ctataactcg cctagtgaca ggggcccaga ggccccccga cccagctcct
     7261 gctccactgg gggccccggg gctccccaat ggcctccttt caggagatga agacttctcc
     7321 tccattgcgg acatggactt ctcagccctg ctgagtcaga tcagctccca attgtaactg
     7381 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     7441 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     7501 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     7561 aagacaatag caggcatgct ggggatgcgg tgggctctat ggctagccgc tgcaagaact
     7621 cttcctcacg gatccgaagt tcctattccg aagttcctat tcttcaaata gtataggaac
     7681 ttcagcgctc actggccgtc gttttacaaa gggcgaattc actggccgtc gttttacaac
     7741 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7801 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7861 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7921 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     7981 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     8041 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8101 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8161 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8221 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8281 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     8341 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     8401 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     8461 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     8521 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     8581 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     8641 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8701 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8761 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8821 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8881 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8941 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     9001 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     9061 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     9121 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9181 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9241 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9301 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9361 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9421 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9481 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9541 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9601 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9661 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9721 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9781 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9841 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9901 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9961 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    10021 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    10081 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    10141 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10201 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    10261 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    10321 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10381 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    10441 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    10501 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    10561 gcgcagcgag tcagtgagcg aggaagcgga ag
//
