LOCUS       sequence-46172-e         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Kato HE, Zhang F, Yizhar O, Ramakrishnan C, Nishizawa T, Hirata K, 
            Ito J, Aita Y, Tsukazaki T, Hayashi S, Hegemann P, Maturana AD, 
            Ishitani R, Deisseroth K, Nureki O
  TITLE     Crystal structure of the channelrhodopsin light-gated cation 
            channel.
  JOURNAL   Nature. 2012 Jan 22. doi: 10.1038/nature10870.
  PUBMED    22266941
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2012 Jan 22. doi: 10.1038/nature10870."
COMMENT     Sequence Label: pLenti-CaMKIIa-C1C2-TS-EYFP
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..304)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(95..116)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     444..463
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaattttgta atccagaggt tgattatcga taagcttgat atcgaattct tacttgtaca
       61 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
      121 cgcgcttctc gttggggtct ttgctcaggg cggactggta gctcaggtag tggttgtcgg
      181 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
      241 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
      301 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
      361 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
      421 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
      481 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
      541 cggggtagcg ggcgaagcac tgcaggccgt agccgaaggt ggtcacgagg gtgggccagg
      601 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg ta
//
