LOCUS       sequence-46174-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt9A-V5
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..62)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actcattact aaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta
       61 ccttcgaacc gcgggccctc tagatcaacc actttgtaca agaaagctgg gtcggcgcgc
      121 ccacccttca gaatgcagcc cttgcaggtg tagacctcct cacgctgcgt gcactgcctg
      181 cactccacat agcagcacca acgcacctgg cactggcagg gccttgtcac cacccggctc
      241 tgtgtgttat ggccgcggcc acagcagatg ctctcgcagt tcttctcacg gtggcaccta
      301 cggccagcgg tgcccgggga gaagcggcca gccaggcaga agctaggcga gtcatccagg
      361 tgcaccagct ctggagtgcg gggcagcggg tcgctgccac ctgcccccga ggcacggccc
      421 cgtggtgggg agatggcacc tgcctcgccg gcagcttcat tggtggtgct gcccaccttg
      481 agtgccgtct catacttgtg cttcagatgc ttgcccacct catggaaagg cgccaactgc
      541 cgccagcagg tccgcaccgt gcatgagcct gacacgccgt ggcacttgca ggtggtctcc
      601 accccagcct tgatcacctt cacacccacg aggttgttgt ggaagtccac acgggctcgc
      661 agatccttgc ttgaccgtct gcccaggaac tccttgacga acttgctgct gtacttaagg
      721 ttgtctccgc agccccccca ctgccaggcc tcacggttct ccaggtcggg tgcctcatcg
      781 caggtacagc gctccatgcg gcccgcgctg cacgccttgg ccagtgcgtg cgtcaggcca
      841 gccgaggaga tggcata
//
