LOCUS       sequence-461742-       15366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template for multi-nucleosome array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15366)
  AUTHORS   Le TT, Gao X, Park SH, Lee J, Inman JT, Lee JH, Killian JL, Badman 
            RP, Berger JM, Wang MD
  TITLE     Synergistic Coordination of Chromatin Torsional Mechanics and 
            Topoisomerase Activity.
  JOURNAL   Cell. 2019 Oct 17;179(3):619-631.e15. doi: 
            10.1016/j.cell.2019.09.034.
  PUBMED    31626768
REFERENCE   2  (bases 1 to 15366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.09"; journalName: "Cell"; date: "2019-10-17- 
            17"; volume: "179"; issue: "3"; pages: "619-631.e15"
COMMENT     Sequence Label: pMDW108
FEATURES             Location/Qualifiers
     source          1..15366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     701..718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    835..856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(871..876,877..894,895..901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    909..925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     914..936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     933..949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     933..949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13699..13716)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13699..13715)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13708..13730)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14043..14065
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(14103..14121)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        14189..14293
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14294..14362,14363..15154)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14512..14531)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(15325..15366,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      961 caagcttgca tgcctgcagg tcgactctag agaggtacct tcaccaggag ggccacccgg
     1021 gcgtacaatc tgctctgatg ccgcatagtt aagcccaccc cgggcgccgc cctggagaat
     1081 cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta
     1141 cgcgctgtcc cccgcgtttt aaccgccaag gggattactc cctagtctcc aggcacgtgt
     1201 cagatatata catcctgtgc atgtattgaa cacaccccct aacacactac gacaccccgg
     1261 gcgccgccct ggagaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca
     1321 ccgcttaaac gcacgtacgc gctgtccccc gcgttttaac cgccaagggg attactccct
     1381 agtctccagg cacgtgtcag atatatacat cctgtgcatg tattgaacac accccctaac
     1441 acactacgac accccgggcg ccgccctgga gaatcccggt gccgaggccg ctcaattggt
     1501 cgtagacagc tctagcaccg cttaaacgca cgtacgcgct gtcccccgcg ttttaaccgc
     1561 caaggggatt actccctagt ctccaggcac gtgtcagata tatacatcct gtgcatgtat
     1621 tgaacacacc ccctaacaca ctacgacacc ccgggcgccg ccctggagaa tcccggtgcc
     1681 gaggccgctc aattggtcgt agacagctct agcaccgctt aaacgcacgt acgcgctgtc
     1741 ccccgcgttt taaccgccaa ggggattact ccctagtctc caggcacgtg tcagatatat
     1801 acatcctgtg catgtattga acacaccccc taacacacta cgacaccccg ggcgccgccc
     1861 tggagaatcc cggtgccgag gccgctcaat tggtcgtaga cagctctagc accgcttaaa
     1921 cgcacgtacg cgctgtcccc cgcgttttaa ccgccaaggg gattactccc tagtctccag
     1981 gcacgtgtca gatatataca tcctgtgcat gtattgaaca caccccctaa cacactacga
     2041 caccccgggc gccgccctgg agaatcccgg tgccgaggcc gctcaattgg tcgtagacag
     2101 ctctagcacc gcttaaacgc acgtacgcgc tgtcccccgc gttttaaccg ccaaggggat
     2161 tactccctag tctccaggca cgtgtcagat atatacatcc tgtgcatgta ttgaacacac
     2221 cccctaacac actacgacac cccgggcgcc gccctggaga atcccggtgc cgaggccgct
     2281 caattggtcg tagacagctc tagcaccgct taaacgcacg tacgcgctgt cccccgcgtt
     2341 ttaaccgcca aggggattac tccctagtct ccaggcacgt gtcagatata tacatcctgt
     2401 gcatgtattg aacacacccc ctaacacact acgacacccc gggcgccgcc ctggagaatc
     2461 ccggtgccga ggccgctcaa ttggtcgtag acagctctag caccgcttaa acgcacgtac
     2521 gcgctgtccc ccgcgtttta accgccaagg ggattactcc ctagtctcca ggcacgtgtc
     2581 agatatatac atcctgtgca tgtattgaac acacccccta acacactacg acaccccggg
     2641 cgccgccctg gagaatcccg gtgccgaggc cgctcaattg gtcgtagaca gctctagcac
     2701 cgcttaaacg cacgtacgcg ctgtcccccg cgttttaacc gccaagggga ttactcccta
     2761 gtctccaggc acgtgtcaga tatatacatc ctgtgcatgt attgaacaca ccccctaaca
     2821 cactacgaca ccccgggcgc cgccctggag aatcccggtg ccgaggccgc tcaattggtc
     2881 gtagacagct ctagcaccgc ttaaacgcac gtacgcgctg tcccccgcgt tttaaccgcc
     2941 aaggggatta ctccctagtc tccaggcacg tgtcagatat atacatcctg tgcatgtatt
     3001 gaacacaccc cctaacacac tacgacaccc cgggcgccgc cctggagaat cccggtgccg
     3061 aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta cgcgctgtcc
     3121 cccgcgtttt aaccgccaag gggattactc cctagtctcc aggcacgtgt cagatatata
     3181 catcctgtgc atgtattgaa cacaccccct aacacactac gacaccccgg gcgccgccct
     3241 ggagaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca ccgcttaaac
     3301 gcacgtacgc gctgtccccc gcgttttaac cgccaagggg attactccct agtctccagg
     3361 cacgtgtcag atatatacat cctgtgcatg tattgaacac accccctaac acactacgac
     3421 accccgggcg ccgccctgga gaatcccggt gccgaggccg ctcaattggt cgtagacagc
     3481 tctagcaccg cttaaacgca cgtacgcgct gtcccccgcg ttttaaccgc caaggggatt
     3541 actccctagt ctccaggcac gtgtcagata tatacatcct gtgcatgtat tgaacacacc
     3601 ccctaacaca ctacgacacc ccgggcgccg ccctggagaa tcccggtgcc gaggccgctc
     3661 aattggtcgt agacagctct agcaccgctt aaacgcacgt acgcgctgtc ccccgcgttt
     3721 taaccgccaa ggggattact ccctagtctc caggcacgtg tcagatatat acatcctgtg
     3781 catgtattga acacaccccc taacacacta cgacaccccg ggcgccgccc tggagaatcc
     3841 cggtgccgag gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg
     3901 cgctgtcccc cgcgttttaa ccgccaaggg gattactccc tagtctccag gcacgtgtca
     3961 gatatataca tcctgtgcat gtattgaaca caccccctaa cacactacga caccccgggc
     4021 gccgccctgg agaatcccgg tgccgaggcc gctcaattgg tcgtagacag ctctagcacc
     4081 gcttaaacgc acgtacgcgc tgtcccccgc gttttaaccg ccaaggggat tactccctag
     4141 tctccaggca cgtgtcagat atatacatcc tgtgcatgta ttgaacacac cccctaacac
     4201 actacgacac cccgggcgcc gccctggaga atcccggtgc cgaggccgct caattggtcg
     4261 tagacagctc tagcaccgct taaacgcacg tacgcgctgt cccccgcgtt ttaaccgcca
     4321 aggggattac tccctagtct ccaggcacgt gtcagatata tacatcctgt gcatgtattg
     4381 aacacacccc ctaacacact acgacacccc gggcgccgcc ctggagaatc ccggtgccga
     4441 ggccgctcaa ttggtcgtag acagctctag caccgcttaa acgcacgtac gcgctgtccc
     4501 ccgcgtttta accgccaagg ggattactcc ctagtctcca ggcacgtgtc agatatatac
     4561 atcctgtgca tgtattgaac acacccccta acacactacg acaccccggg cgccgccctg
     4621 gagaatcccg gtgccgaggc cgctcaattg gtcgtagaca gctctagcac cgcttaaacg
     4681 cacgtacgcg ctgtcccccg cgttttaacc gccaagggga ttactcccta gtctccaggc
     4741 acgtgtcaga tatatacatc ctgtgcatgt attgaacaca ccccctaaca cactacgaca
     4801 ccccgggcgc cgccctggag aatcccggtg ccgaggccgc tcaattggtc gtagacagct
     4861 ctagcaccgc ttaaacgcac gtacgcgctg tcccccgcgt tttaaccgcc aaggggatta
     4921 ctccctagtc tccaggcacg tgtcagatat atacatcctg tgcatgtatt gaacacaccc
     4981 cctaacacac tacgacaccc cgggcgccgc cctggagaat cccggtgccg aggccgctca
     5041 attggtcgta gacagctcta gcaccgctta aacgcacgta cgcgctgtcc cccgcgtttt
     5101 aaccgccaag gggattactc cctagtctcc aggcacgtgt cagatatata catcctgtgc
     5161 atgtattgaa cacaccccct aacacactac gacaccccgg gcgccgccct ggagaatccc
     5221 ggtgccgagg ccgctcaatt ggtcgtagac agctctagca ccgcttaaac gcacgtacgc
     5281 gctgtccccc gcgttttaac cgccaagggg attactccct agtctccagg cacgtgtcag
     5341 atatatacat cctgtgcatg tattgaacac accccctaac acactacgac accccgggcg
     5401 ccgccctgga gaatcccggt gccgaggccg ctcaattggt cgtagacagc tctagcaccg
     5461 cttaaacgca cgtacgcgct gtcccccgcg ttttaaccgc caaggggatt actccctagt
     5521 ctccaggcac gtgtcagata tatacatcct gtgcatgtat tgaacacacc ccctaacaca
     5581 ctacgacacc ccgggcgccg ccctggagaa tcccggtgcc gaggccgctc aattggtcgt
     5641 agacagctct agcaccgctt aaacgcacgt acgcgctgtc ccccgcgttt taaccgccaa
     5701 ggggattact ccctagtctc caggcacgtg tcagatatat acatcctgtg catgtattga
     5761 acacaccccc taacacacta cgacaccccg ggcgccgccc tggagaatcc cggtgccgag
     5821 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg cgctgtcccc
     5881 cgcgttttaa ccgccaaggg gattactccc tagtctccag gcacgtgtca gatatataca
     5941 tcctgtgcat gtattgaaca caccccctaa cacactacga caccccgggc gccgccctgg
     6001 agaatcccgg tgccgaggcc gctcaattgg tcgtagacag ctctagcacc gcttaaacgc
     6061 acgtacgcgc tgtcccccgc gttttaaccg ccaaggggat tactccctag tctccaggca
     6121 cgtgtcagat atatacatcc tgtgcatgta ttgaacacac cccctaacac actacgacac
     6181 cccgggcgcc gccctggaga atcccggtgc cgaggccgct caattggtcg tagacagctc
     6241 tagcaccgct taaacgcacg tacgcgctgt cccccgcgtt ttaaccgcca aggggattac
     6301 tccctagtct ccaggcacgt gtcagatata tacatcctgt gcatgtattg aacacacccc
     6361 ctaacacact acgacacccc gggcgccgcc ctggagaatc ccggtgccga ggccgctcaa
     6421 ttggtcgtag acagctctag caccgcttaa acgcacgtac gcgctgtccc ccgcgtttta
     6481 accgccaagg ggattactcc ctagtctcca ggcacgtgtc agatatatac atcctgtgca
     6541 tgtattgaac acacccccta acacactacg acaccccggg cgccgccctg gagaatcccg
     6601 gtgccgaggc cgctcaattg gtcgtagaca gctctagcac cgcttaaacg cacgtacgcg
     6661 ctgtcccccg cgttttaacc gccaagggga ttactcccta gtctccaggc acgtgtcaga
     6721 tatatacatc ctgtgcatgt attgaacaca ccccctaaca cactacgaca ccccgggcgc
     6781 cgccctggag aatcccggtg ccgaggccgc tcaattggtc gtagacagct ctagcaccgc
     6841 ttaaacgcac gtacgcgctg tcccccgcgt tttaaccgcc aaggggatta ctccctagtc
     6901 tccaggcacg tgtcagatat atacatcctg tgcatgtatt gaacacaccc cctaacacac
     6961 tacgacaccc cgggcgccgc cctggagaat cccggtgccg aggccgctca attggtcgta
     7021 gacagctcta gcaccgctta aacgcacgta cgcgctgtcc cccgcgtttt aaccgccaag
     7081 gggattactc cctagtctcc aggcacgtgt cagatatata catcctgtgc atgtattgaa
     7141 cacaccccct aacacactac gacaccccgg gcgccgccct ggagaatccc ggtgccgagg
     7201 ccgctcaatt ggtcgtagac agctctagca ccgcttaaac gcacgtacgc gctgtccccc
     7261 gcgttttaac cgccaagggg attactccct agtctccagg cacgtgtcag atatatacat
     7321 cctgtgcatg tattgaacac accccctaac acactacgac accccgggcg ccgccctgga
     7381 gaatcccggt gccgaggccg ctcaattggt cgtagacagc tctagcaccg cttaaacgca
     7441 cgtacgcgct gtcccccgcg ttttaaccgc caaggggatt actccctagt ctccaggcac
     7501 gtgtcagata tatacatcct gtgcatgtat tgaacacacc ccctaacaca ctacgacacc
     7561 ccgggcgccg ccctggagaa tcccggtgcc gaggccgctc aattggtcgt agacagctct
     7621 agcaccgctt aaacgcacgt acgcgctgtc ccccgcgttt taaccgccaa ggggattact
     7681 ccctagtctc caggcacgtg tcagatatat acatcctgtg catgtattga acacaccccc
     7741 taacacacta cgacaccccg ggcgccgccc tggagaatcc cggtgccgag gccgctcaat
     7801 tggtcgtaga cagctctagc accgcttaaa cgcacgtacg cgctgtcccc cgcgttttaa
     7861 ccgccaaggg gattactccc tagtctccag gcacgtgtca gatatataca tcctgtgcat
     7921 gtattgaaca caccccctaa cacactacga caccccgggc gccgccctgg agaatcccgg
     7981 tgccgaggcc gctcaattgg tcgtagacag ctctagcacc gcttaaacgc acgtacgcgc
     8041 tgtcccccgc gttttaaccg ccaaggggat tactccctag tctccaggca cgtgtcagat
     8101 atatacatcc tgtgcatgta ttgaacacac cccctaacac actacgacac cccgggcgcc
     8161 gccctggaga atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
     8221 taaacgcacg tacgcgctgt cccccgcgtt ttaaccgcca aggggattac tccctagtct
     8281 ccaggcacgt gtcagatata tacatcctgt gcatgtattg aacacacccc ctaacacact
     8341 acgacacccc gggcgccgcc ctggagaatc ccggtgccga ggccgctcaa ttggtcgtag
     8401 acagctctag caccgcttaa acgcacgtac gcgctgtccc ccgcgtttta accgccaagg
     8461 ggattactcc ctagtctcca ggcacgtgtc agatatatac atcctgtgca tgtattgaac
     8521 acacccccta acacactacg acaccccggg cgccgccctg gagaatcccg gtgccgaggc
     8581 cgctcaattg gtcgtagaca gctctagcac cgcttaaacg cacgtacgcg ctgtcccccg
     8641 cgttttaacc gccaagggga ttactcccta gtctccaggc acgtgtcaga tatatacatc
     8701 ctgtgcatgt attgaacaca ccccctaaca cactacgaca ccccgggcgc cgccctggag
     8761 aatcccggtg ccgaggccgc tcaattggtc gtagacagct ctagcaccgc ttaaacgcac
     8821 gtacgcgctg tcccccgcgt tttaaccgcc aaggggatta ctccctagtc tccaggcacg
     8881 tgtcagatat atacatcctg tgcatgtatt gaacacaccc cctaacacac tacgacaccc
     8941 cgggcgccgc cctggagaat cccggtgccg aggccgctca attggtcgta gacagctcta
     9001 gcaccgctta aacgcacgta cgcgctgtcc cccgcgtttt aaccgccaag gggattactc
     9061 cctagtctcc aggcacgtgt cagatatata catcctgtgc atgtattgaa cacaccccct
     9121 aacacactac gacaccccgg gcgccgccct ggagaatccc ggtgccgagg ccgctcaatt
     9181 ggtcgtagac agctctagca ccgcttaaac gcacgtacgc gctgtccccc gcgttttaac
     9241 cgccaagggg attactccct agtctccagg cacgtgtcag atatatacat cctgtgcatg
     9301 tattgaacac accccctaac acactacgac accccgggcg ccgccctgga gaatcccggt
     9361 gccgaggccg ctcaattggt cgtagacagc tctagcaccg cttaaacgca cgtacgcgct
     9421 gtcccccgcg ttttaaccgc caaggggatt actccctagt ctccaggcac gtgtcagata
     9481 tatacatcct gtgcatgtat tgaacacacc ccctaacaca ctacgacacc ccgggcgccg
     9541 ccctggagaa tcccggtgcc gaggccgctc aattggtcgt agacagctct agcaccgctt
     9601 aaacgcacgt acgcgctgtc ccccgcgttt taaccgccaa ggggattact ccctagtctc
     9661 caggcacgtg tcagatatat acatcctgtg catgtattga acacaccccc taacacacta
     9721 cgacaccccg ggcgccgccc tggagaatcc cggtgccgag gccgctcaat tggtcgtaga
     9781 cagctctagc accgcttaaa cgcacgtacg cgctgtcccc cgcgttttaa ccgccaaggg
     9841 gattactccc tagtctccag gcacgtgtca gatatataca tcctgtgcat gtattgaaca
     9901 caccccctaa cacactacga caccccgggc gccgccctgg agaatcccgg tgccgaggcc
     9961 gctcaattgg tcgtagacag ctctagcacc gcttaaacgc acgtacgcgc tgtcccccgc
    10021 gttttaaccg ccaaggggat tactccctag tctccaggca cgtgtcagat atatacatcc
    10081 tgtgcatgta ttgaacacac cccctaacac actacgacac cccgggcgcc gccctggaga
    10141 atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct taaacgcacg
    10201 tacgcgctgt cccccgcgtt ttaaccgcca aggggattac tccctagtct ccaggcacgt
    10261 gtcagatata tacatcctgt gcatgtattg aacacacccc ctaacacact acgacacccc
    10321 gggcgccgcc ctggagaatc ccggtgccga ggccgctcaa ttggtcgtag acagctctag
    10381 caccgcttaa acgcacgtac gcgctgtccc ccgcgtttta accgccaagg ggattactcc
    10441 ctagtctcca ggcacgtgtc agatatatac atcctgtgca tgtattgaac acacccccta
    10501 acacactacg acaccccggg cgccgccctg gagaatcccg gtgccgaggc cgctcaattg
    10561 gtcgtagaca gctctagcac cgcttaaacg cacgtacgcg ctgtcccccg cgttttaacc
    10621 gccaagggga ttactcccta gtctccaggc acgtgtcaga tatatacatc ctgtgcatgt
    10681 attgaacaca ccccctaaca cactacgaca ccccgggcgc cgccctggag aatcccggtg
    10741 ccgaggccgc tcaattggtc gtagacagct ctagcaccgc ttaaacgcac gtacgcgctg
    10801 tcccccgcgt tttaaccgcc aaggggatta ctccctagtc tccaggcacg tgtcagatat
    10861 atacatcctg tgcatgtatt gaacacaccc cctaacacac tacgacaccc cgggcgccgc
    10921 cctggagaat cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta
    10981 aacgcacgta cgcgctgtcc cccgcgtttt aaccgccaag gggattactc cctagtctcc
    11041 aggcacgtgt cagatatata catcctgtgc atgtattgaa cacaccccct aacacactac
    11101 gacaccccgg gcgccgccct ggagaatccc ggtgccgagg ccgctcaatt ggtcgtagac
    11161 agctctagca ccgcttaaac gcacgtacgc gctgtccccc gcgttttaac cgccaagggg
    11221 attactccct agtctccagg cacgtgtcag atatatacat cctgtgcatg tattgaacac
    11281 accccctaac acactacgac accccgggcg ccgccctgga gaatcccggt gccgaggccg
    11341 ctcaattggt cgtagacagc tctagcaccg cttaaacgca cgtacgcgct gtcccccgcg
    11401 ttttaaccgc caaggggatt actccctagt ctccaggcac gtgtcagata tatacatcct
    11461 gtgcatgtat tgaacacacc ccctaacaca ctacgacacc ccgggcgccg ccctggagaa
    11521 tcccggtgcc gaggccgctc aattggtcgt agacagctct agcaccgctt aaacgcacgt
    11581 acgcgctgtc ccccgcgttt taaccgccaa ggggattact ccctagtctc caggcacgtg
    11641 tcagatatat acatcctgtg catgtattga acacaccccc taacacacta cgacaccccg
    11701 ggcgccgccc tggagaatcc cggtgccgag gccgctcaat tggtcgtaga cagctctagc
    11761 accgcttaaa cgcacgtacg cgctgtcccc cgcgttttaa ccgccaaggg gattactccc
    11821 tagtctccag gcacgtgtca gatatataca tcctgtgcat gtattgaaca caccccctaa
    11881 cacactacga caccccgggc gccgccctgg agaatcccgg tgccgaggcc gctcaattgg
    11941 tcgtagacag ctctagcacc gcttaaacgc acgtacgcgc tgtcccccgc gttttaaccg
    12001 ccaaggggat tactccctag tctccaggca cgtgtcagat atatacatcc tgtgcatgta
    12061 ttgaacacac cccctaacac actacgacac cccgggcgcc gccctggaga atcccggtgc
    12121 cgaggccgct caattggtcg tagacagctc tagcaccgct taaacgcacg tacgcgctgt
    12181 cccccgcgtt ttaaccgcca aggggattac tccctagtct ccaggcacgt gtcagatata
    12241 tacatcctgt gcatgtattg aacacacccc ctaacacact acgacacccc gggcgccgcc
    12301 ctggagaatc ccggtgccga ggccgctcaa ttggtcgtag acagctctag caccgcttaa
    12361 acgcacgtac gcgctgtccc ccgcgtttta accgccaagg ggattactcc ctagtctcca
    12421 ggcacgtgtc agatatatac atcctgtgca tgtattgaac acacccccta acacactacg
    12481 acaccccggg cgccgccctg gagaatcccg gtgccgaggc cgctcaattg gtcgtagaca
    12541 gctctagcac cgcttaaacg cacgtacgcg ctgtcccccg cgttttaacc gccaagggga
    12601 ttactcccta gtctccaggc acgtgtcaga tatatacatc ctgtgcatgt attgaacaca
    12661 ccccctaaca cactacgaca ccccgggcgc cgccctggag aatcccggtg ccgaggccgc
    12721 tcaattggtc gtagacagct ctagcaccgc ttaaacgcac gtacgcgctg tcccccgcgt
    12781 tttaaccgcc aaggggatta ctccctagtc tccaggcacg tgtcagatat atacatcctg
    12841 tgcatgtatt gaacacaccc cctaacacac tacgacaccc cgggcgccgc cctggagaat
    12901 cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta
    12961 cgcgctgtcc cccgcgtttt aaccgccaag gggattactc cctagtctcc aggcacgtgt
    13021 cagatatata catcctgtgc atgtattgaa cacaccccct aacacactac gacaccccgg
    13081 gcgccgccct ggagaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca
    13141 ccgcttaaac gcacgtacgc gctgtccccc gcgttttaac cgccaagggg attactccct
    13201 agtctccagg cacgtgtcag atatatacat cctgtgcatg tattgaacac accccctaac
    13261 acactacgac accccgggcg ccgccctgga gaatcccggt gccgaggccg ctcaattggt
    13321 cgtagacagc tctagcaccg cttaaacgca cgtacgcgct gtcccccgcg ttttaaccgc
    13381 caaggggatt actccctagt ctccaggcac gtgtcagata tatacatcct gtgcatgtat
    13441 tgaacacacc ccctaacaca ctacgacacc ccgggcgccg ccctggagaa tcccggtgcc
    13501 gaggccgctc aattggtcgt agacagctct agcaccgctt aaacgcacgt acgcgctgtc
    13561 ccccgcgttt taaccgccaa ggggattact ccctagtctc caggcacgtg tcagatatat
    13621 acatcctgtg catgtattga acacaccccc taacacacta cgacaccccg tgggccacat
    13681 cggtgggatc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    13741 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    13801 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
    13861 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
    13921 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
    13981 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
    14041 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
    14101 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
    14161 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    14221 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    14281 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    14341 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    14401 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    14461 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    14521 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
    14581 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    14641 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    14701 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    14761 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    14821 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    14881 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    14941 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    15001 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    15061 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    15121 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    15181 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    15241 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    15301 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    15361 gcaaac
//
