LOCUS       sequence-461750-        9134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  M13 helper genes cloned into the pSEVA631 vector. This plasmid does 
            not carry an M13 packaging signal. (This plasmid is a gift of the 
            Jaramillo Lab, where it is called PAJ296.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Kushwaha M, Singh V, Jaramillo A
  TITLE     Development of a genetically-encoded in vivo computing platform to 
            play the game of Tic-Tac-Toe
  JOURNAL   Zenodo (2017) Conference: SB7.0: The Seventh International Meeting 
            on Synthetic Biology.
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zenodo 
            (2017) Conference: SB7.0: The Seventh International Meeting on 
            Synthetic Biology."
COMMENT     Sequence Label: pSEVA47_Helper
FEATURES             Location/Qualifiers
     source          1..9134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             843..1106
                     /codon_start=1
                     /gene="V"
                     /product="pV"
                     /label=M13 gene V
                     /note="replication"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             1108..1209
                     /codon_start=1
                     /gene="VII"
                     /product="pVII"
                     /label=M13 gene VII
                     /note="minor coat protein"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             1206..1304
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             1301..1522
                     /codon_start=1
                     /gene="VIII"
                     /product="pVIII"
                     /label=M13 gene VIII
                     /note="major coat protein"
                     /translation="MKKSLALKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEYI
                     GYAWAMVVVIVGATIGIKLFKKFTSKAS"
     terminator      1526..1574
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             1579..2853
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             2856..3194
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             3196..4242
                     /codon_start=1
                     /gene="I"
                     /product="pI"
                     /label=M13 gene I
                     /note="phage assembly"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             4220..5500
                     /codon_start=1
                     /gene="IV"
                     /product="pIV"
                     /label=M13 gene IV
                     /note="phage assembly"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     terminator      5573..5667
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             5903..6694
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            6840..6948
                     /note="incP origin of transfer"
     CDS             complement(7139..8089)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      8137..8359
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(8460..8546)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     8561..8583
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             join(8733..9134,1..831)
                     /codon_start=1
                     /gene="II"
                     /product="pII"
                     /label=M13 gene II
                     /note="replication"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSAR
                     TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
ORIGIN
        1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
      421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
      841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
      901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
      961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagccct
     1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     1561 tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
     1621 tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
     1681 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt
     1741 ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
     1801 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     1861 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     1921 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     1981 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     2041 cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
     2101 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     2161 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag
     2221 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
     2281 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
     2341 ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
     2401 gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     2461 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     2521 gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
     2581 ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
     2641 ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
     2701 tttgtctttg gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
     2761 ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
     2821 tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
     2881 tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
     2941 ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
     3001 ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
     3061 ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
     3121 tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
     3181 attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
     3241 ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
     3301 cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
     3361 cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
     3421 tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
     3481 acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
     3541 aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
     3601 cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
     3661 tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
     3721 gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
     3781 actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
     3841 tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
     3901 aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
     3961 tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
     4021 gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
     4081 cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
     4141 agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
     4201 attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
     4261 tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
     4321 tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
     4381 tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
     4441 tgttttacgt gcaaataatt ttgatatggt aggttctaac ccttccatta ttcagaagta
     4501 taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
     4561 tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
     4621 ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
     4681 gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
     4741 tagtgctcct aaagatattt tagataacct tcctcaattc ctttcaactg ttgatttgcc
     4801 aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
     4861 tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
     4921 cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
     4981 agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
     5041 tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
     5101 tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
     5161 tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
     5221 tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
     5281 tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
     5341 cggtggcctc actgattata aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa
     5401 aatcccttta atcggcctcc tgtttagctc ccgctctgat tctaacgagg aaagcacgtt
     5461 atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgc
     5521 atgcaagctt gcggccgcgt cgtgactggg aaaaccctgg cgactagtct tggactcctg
     5581 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
     5641 cgccgggcgt tttttattgg tgagaatcca ggggtcccca ataattacga tttacgtatt
     5701 taaatgaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     5761 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     5821 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     5881 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
     5941 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     6001 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     6061 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     6121 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     6181 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     6241 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     6301 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     6361 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     6421 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     6481 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     6541 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     6601 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     6661 gaaaggcgag atcaccaagg tagtcggcaa ataagacaat tgtccttttc cgctgcataa
     6721 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     6781 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     6841 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     6901 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgta ggccggcctg
     6961 gtgaacagct ttgaatgcac caaaaactcg taaaagctct gatgtatcta tcttttttac
     7021 accgttttca tctgtgcata tggacagttt tccctttgat atgtaacggt gaacagttgt
     7081 tctacttttg tttgttagtc ttgatgcttc actgatagat acaagagcca taagaacctc
     7141 agatccttcc gtatttagcc agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc
     7201 atgagaacga accattgaga tcatacttac tttgcatgtc actcaaaaat tttgcctcaa
     7261 aactggtgag ctgaattttt gctgttaaag catcgtgtag tgtttttctt agtccgttat
     7321 gtaggtagga atctgatgta atggttgttg gtattttgtc accattcatt tttatctggt
     7381 tgttctcaag ttcggttacg agatccattt gtctatctag ttcaacttgg aaaatcaacg
     7441 tatcagtcgg gcggcctcgc ttatcaacca ccaatttcat attgctgtaa gtgtttaaat
     7501 ctttacttat tggtttcaaa acccattggt taagcctttt aaactcatgg tagttatttt
     7561 caagcattaa catgaactta aattcatcaa ggctaatctc tatatttgcc ttgtgagttt
     7621 tcttttgtgt tagttctttt aataaccact cataaatcct catagagtat ttgttttcaa
     7681 aagacttaac atgttccaga ttatatttta tgaatttttt taactggaaa agataaggca
     7741 atatctcttc actaaaaact aattctaatt tttcgcttga gaacttggca tagtttgtcc
     7801 actggaagat cttaaagcct ttaaccaaag gattcctgat ttccacagtt ctcgtcatca
     7861 gctctctggt tgctttagct aatacaccat aagcattttc cctactgatg ttcatcatct
     7921 gagcgtattg gttataagtg aacgataccg tccgttcttt ccttgtaggg ttttcaatcg
     7981 tggggttgag tagtgccaca cagcataaaa ttagcttggt ttcatgctcc gttaagtcat
     8041 agcgactaat cgctagttca tttgctttga aaacaactaa ttcagacata catctcaatt
     8101 ggtctaggtg attttaatca ctataccaat tgagatgggc tagtcaatga taattactag
     8161 tccttttcct ttgagttgtg ggtatctgta aattctgcta gacctttgct ggaaaacttg
     8221 taaattctgc tagaccctct gtaaattccg ctagaccttt gtgtgttttt tttgtttata
     8281 ttcaagtggt tataatttat agaataaaga aagaataaaa aaagataaaa agaatagatc
     8341 ccagccctgt gtataactca ctactttagt cagttccgca gtattacaaa aggatgtcgc
     8401 aaacgctgtt tgctcctcta caaaacagac ctggcgcgcc cagctgtcta gggcggcgga
     8461 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     8521 ttcgactgag cctttcgttt tatttgatgc ctttaattaa agcggataac aatttcacac
     8581 aggaggccgc ctaggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8641 attttaacaa aatattaacg tttacaattt aaatatttgc ttatacaatc ttcctgtttt
     8701 tggggctttt ctgattatca accggggtac atatgattga catgctagtt ttacgattac
     8761 cgttcatcga ttctcttgtt tgctccagac tctcaggcaa tgacctgata gcctttgtag
     8821 acctctcaaa aatagctacc ctctccggca tgaatttatc agctagaacg gttgaatatc
     8881 atattgatgg tgatttgact gtctccggcc tttctcaccc ttttgaatct ttacctacac
     8941 attactcagg cattgcattt aaaatatatg agggttctaa aaatttttat ccttgcgttg
     9001 aaataaaggc ttctcccgca aaagtattac agggtcataa tgtttttggt acaaccgatt
     9061 tagctttatg ctctgaggct ttattgctta attttgctaa ttctttgcct tgcctgtatg
     9121 atttattgga tgtt
//
