LOCUS       sequence-46176-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt10A-V5
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..48)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg
       61 gccctctaga tcaaccactt tgtacaagaa agctgggtcg gcgcgcccac ccttcagaat
      121 gcacttgcag acgctgaccc actcggtgat gcggcactct tcgcagacca cgaaacagca
      181 ccagtggaag cggcagtggc agcgctcgct gcgcgtctgg cgcaggatgt tgtggccgcg
      241 gccgcagcac atgctgccgc agccatccga gccggcgctg ctcttgttgc acaggcggcc
      301 cacggtgccc gccgagtcca ggcgcggctc gcgctcgcag aagtcgggag acttttcgaa
      361 gtagaccagg tcggcggggc tggcccgtcg gcgcggcccg ggagcgcccg gagccggcga
      421 gggtgccccc gctgggcccg gctccagctg gccgccgttg cggttgtgcg gccggatgag
      481 cgtggcgcgg tggaagcggc tgcgcagcag cgcccccacg gtgcggaact cgggcgtcac
      541 ctgccagcac gtcttgagct ggcagctgcc tgacgtgccg tggcacttgc acttccgccg
      601 catgttctcc atcactgcct gcctcccaac tcggttgttg tgaagcctca ttctcgcgtg
      661 gatgtctctg tgaggctccc gggagtccag aaagtcctta gaaaagcgct ccccgaagcc
      721 catgtcgggg ctgcagccgc cccactccca ggagtcctgc aggcctgggc tggctgtggg
      781 cagggctggg tgttccggga ccccatggc
//
