LOCUS       sequence-461768-        8435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  KHC064 (pX335 BRD2 K1-1a), gRNA and cas9D10A mammalian expression 
            vector for CRISPR mediated cutting of BRD2 at the N-terminus, use 
            with KHC065.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Bond AG, Craigon C, Chan KH, Testa A, Karapetsas A, Fasimoye R, 
            Macartney T, Blow JJ, Alessi DR, Ciulli A
  TITLE     Development of BromoTag: A ""Bump-and-Hole""-PROTAC System to Induce
            Potent, Rapid, and Selective Degradation of Tagged Target Proteins.
  JOURNAL   J Med Chem. 2021 Oct 28;64(20):15477-15502. doi: 
            10.1021/acs.jmedchem.1c01532. Epub 2021 Oct 15.
  PUBMED    34652918
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.1c01532"; journalName: "J Med Chem"; date: 
            "2021-10-28- 28"; volume: "64"; issue: "20"; pages: "15477-15502"
COMMENT     Sequence Label: KHC064 pX335 BRD2 K1-1a
FEATURES             Location/Qualifiers
     source          1..8435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        120..405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     misc_RNA        complement(502..577)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(599..847)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(657..676)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(827..847)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(909..1497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(989..1008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1668..2459,2460..2528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2291..2310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2529..2633)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2701..2719
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2757..2779)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2879..2898
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2915..3370)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3052..3073)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3264..3283
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3445..3585
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3456..3585
                     /label=AAV2 ITR
     polyA_signal    3594..3801
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3790..3807
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3844..3864)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3868..7941,7942..7944,7945..7968))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3868..7968)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(7978..7998)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8002..8028)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          8049..8276
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     promoter        complement(join(8277..8435,1..118))
                     /label=chicken beta-actin promoter
ORIGIN
        1 cccccccccc ccgcccccat cgctgcacaa aataattaaa aaataaataa atacaaaatt
       61 gggggtgggg agggggggga gatggggaga gtgaagcaga acgtggggct cacctcgacc
      121 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
      181 atgtactggg cacaatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
      241 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
      301 cattgacgtc aatggaaagt ccctattggc gttactattg acgtcaatgg gcgggggtcg
      361 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgggtac ctctagagcc
      421 atttgtctgc agaattggcg cacgcgctaa aaacggacta gccttatttt aacttgctat
      481 ttctagctct aaaacaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac
      541 tagccttatt ttaacttgct atttctagct ctaaaaccaa ggaaccggcg ctgccctcgg
      601 tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg
      661 taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt
      721 attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc
      781 caattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata
      841 ggccctcaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1801 ataccgcggc ttccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2641 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2701 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2761 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     2821 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     2881 gcatcagagc agattgtact gagagtgcac cataaaattg taaacgttaa tattttgtta
     2941 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaatagac cgaaatcggc
     3001 aaaatccctt ataaatcaaa agaatagccc gagatagagt tgagtgttgt tccagtttgg
     3061 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3121 cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc
     3181 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3241 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc taaggcgctg
     3301 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3361 cagggcgcgt actatggttg ctttgacgta tgcggtgtga aataccgcac agatgcgtaa
     3421 ggagaaaata ccgcatcagg cgcccctgca ggcagctgcg cgctcgctcg ctcactgagg
     3481 ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc
     3541 gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc cgctccccag
     3601 catgcctgct attctcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
     3661 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
     3721 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
     3781 gctggcaact agaaggcaca gtcgaggctg atcagcgagc tctaggaatt cttagctggc
     3841 ctccaccttt ctcttcttct tggggctgtc gcctcccagc tgagacaggt cgatccgtgt
     3901 ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct ctttggtgct
     3961 ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag gggctcccag
     4021 attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg gcttatcccg
     4081 gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga tcactctctt
     4141 ggagaactcg ctgatctgct cgatgatctc gtccaggtag tgcttgtgct gttccacaaa
     4201 cagctgtttc tgctcattat cctcggggga gcccttcagc ttctcatagt ggctggccag
     4261 gtacaggaag ttcacatatt tggagggcag ggccagttcg tttcccttct gcagttcgcc
     4321 ggcagaggcc agcattctct tccggccgtt ttccagctcg aacagggagt acttaggcag
     4381 cttgatgatc aggtcctttt tcacttcttt gtagcccttg gcttccagaa agtcgatggg
     4441 attcttctcg aagctgcttc tttccatgat ggtgatcccc agcagctctt tcacactctt
     4501 cagtttcttg gacttgccct tttccacttt ggccaccacc agcacagaat aggccacggt
     4561 ggggctgtcg aagccgccgt acttcttagg gtcccagtcc ttctttctgg cgatcagctt
     4621 atcgctgttc ctcttgggca ggatagactc tttgctgaag ccgcctgtct gcacctcggt
     4681 ctttttcacg atattcactt ggggcatgct cagcactttc cgcacggtgg caaaatcccg
     4741 gcccttatcc cacacgatct ccccggtttc gccgtttgtc tcgatcagag gccgcttccg
     4801 gatctcgccg ttggccaggg taatctcggt cttgaaaaag ttcatgatgt tgctgtagaa
     4861 gaagtacttg gcggtagcct tgccgatttc ctgctcgctc ttggcgatca tcttccgcac
     4921 gtcgtacacc ttgtagtcgc cgtacacgaa ctcgctttcc agcttagggt actttttgat
     4981 cagggcggtt cccacgacgg cgttcaggta ggcgtcgtgg gcgtggtggt agttgttgat
     5041 ctcgcgcact ttgtaaaact ggaaatcctt ccggaaatcg gacaccagct tggacttcag
     5101 ggtgatcact ttcacttccc ggatcagctt gtcattctcg tcgtacttag tgttcatccg
     5161 ggagtccagg atctgtgcca cgtgctttgt gatctgccgg gtttccacca gctgtctctt
     5221 gatgaagccg gccttatcca gttcgctcag gccgcctctc tcggccttgg tcagattgtc
     5281 gaactttctc tgggtaatca gcttggcgtt cagcagctgc cgccagtagt tcttcatctt
     5341 cttcacgacc tcttcggagg gcacgttgtc gctcttgccc cggttcttgt cgcttctggt
     5401 cagcaccttg ttgtcgatgg agtcgtcctt cagaaagctc tgaggcacga tatggtccac
     5461 atcgtagtcg gacagccggt tgatgtccag ttcctggtcc acgtacatat cccgcccatt
     5521 ctgcaggtag tacaggtaca gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc
     5581 tttcaggatc tggctgccca gctctttgat gccctcttcg atccgcttca ttctctcgcg
     5641 gctgttcttc tgtcccttct gggtggtctg gttctctctg gccatttcga tcacgatgtt
     5701 ctcgggcttg tgccggccca tcactttcac gagctcgtcc accaccttca ctgtctgcag
     5761 gatgcccttc ttaatggcgg ggctgccggc cagattggca atgtgctcgt gcaggctatc
     5821 gccctggccg gacacctggg ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg
     5881 gatcagctgc atgaagtttc tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt
     5941 cttgccggac tgcttgtccc ggatgccgtt gatcagcttc cggctcagcc tgccccagcc
     6001 ggtgtatctc cgccgcttca gctgcttcat cactttgtcg tcgaacaggt gggcataggt
     6061 tttcagccgt tcctcgatca tctctctgtc ctcaaacagt gtcagggtca gcacgatatc
     6121 ttccagaatg tcctcgtttt cctcattgtc caggaagtcc ttgtccttga taattttcag
     6181 cagatcgtgg tatgtgccca gggaggcgtt gaaccgatct tccacgccgg agatttccac
     6241 ggagtcgaag cactcgattt tcttgaagta gtcctctttc agctgcttca cggtcacttt
     6301 ccggttggtc ttgaacagca ggtccacgat ggcctttttc tgctcgccgc tcaggaaggc
     6361 gggctttctc attccctcgg tcacgtattt cactttggtc agctcgttat acacggtgaa
     6421 gtactcgtac agcaggctgt gcttgggcag caccttctcg ttgggcaggt tcttatcgaa
     6481 gttggtcatc cgctcgatga agctctgggc ggaagcgccc ttgtccacca cttcctcgaa
     6541 gttccagggg gtgatggttt cctcgctctt tctggtcatc caggcgaatc tgctgtttcc
     6601 cctggccaga gggcccacgt agtaggggat gcggaaggtc aggatcttct cgatcttttc
     6661 ccggttgtcc ttcaggaatg ggtaaaaatc ttcctgccgc cgcagaatgg cgtgcagctc
     6721 tcccaggtgg atctggtggg ggatgctgcc gttgtcgaag gtccgctgct tccgcagcag
     6781 gtcctctctg ttcagcttca cgagcagttc ctcggtgccg tccatctttt ccaggatggg
     6841 cttgatgaac ttgtagaact cttcctggct ggctccgccg tcaatgtagc cggcgtagcc
     6901 gttcttgctc tggtcgaaga aaatctcttt gtacttctca ggcagctgct gccgcacgag
     6961 agctttcagc agggtcaggt cctggtggtg ctcgtcgtat ctcttgatca tagaggcgct
     7021 caggggggcc ttggtgatct cggtgttcac tctcaggatg tcgctcagca ggatggcgtc
     7081 ggacaggttc ttggcggcca gaaacaggtc ggcgtactgg tcgccgatct gggccagcag
     7141 gttgtccagg tcgtcgtcgt aggtgtcctt gctcagctgc agtttggcat cctcggccag
     7201 gtcgaagttg ctcttgaagt tgggggtcag gcccaggctc agggcaatca ggttgccgaa
     7261 caggccattc ttcttctcgc cgggcagctg ggcgatcaga ttttccagcc gtctgctctt
     7321 gctcagtctg gcagacagga tggccttggc gtccacgccg ctggcgttga tggggttttc
     7381 ctcgaacagc tggttgtagg tctgcaccag ctggatgaac agcttgtcca cgtcgctgtt
     7441 gtcggggttc aggtcgccct cgatcaggaa gtggccccgg aacttgatca tgtgggccag
     7501 ggccagatag atcagccgca ggtcggcctt gtcggtgctg tccaccagtt tctttctcag
     7561 gtggtagatg gtggggtact tctcgtggta ggccacctcg tccacgatgt tgccgaagat
     7621 ggggtgccgc tcgtgcttct tatcctcttc caccaggaag gactcttcca gtctgtggaa
     7681 gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat agcagatccg
     7741 gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg cctcggctgt
     7801 ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt gccggtcggt
     7861 gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga tcacggccca
     7921 gcccacagag ttggtgccga tggccaggcc gatgctgtac ttcttgtcgg acgcttcgac
     7981 cttgcgcttt ttcttcggcg aagcgtaatc tggaacatcg tatgggtaca tggtggcacc
     8041 ggtccaacct gaaaaaaagt gatttcaggc aggtgctcca ggtaattaaa cattaatacc
     8101 ccaccaacca accatccctt aaacccttac ctcttgctca gctaattaca gcccggagga
     8161 gaagggccgt cccgcccgct cacctgtggg agtaacgcgg tcagtcagag ccggggcggg
     8221 cggcgcgagg cggcggcgga gcggggcacg gggcgaaggc agcgcgcagc gactcccgcc
     8281 cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc gcctcgccat aaaaggaaac
     8341 tttcggagcg cgccgctctg attggctgcc gccgcacctc tccgcctcgc cccgccccgc
     8401 ccctcgcccc gccccgcccc gcctggcgcg cgccc
//
