LOCUS       sequence-461777-        8395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stably express CD63 fused with GFP in mammalian cells via the 
            sleeping beauty transposon system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Li W, Saleh NA, Gao C, Gagea MA, Vitija X, Kanada M, Deng CX
  TITLE     Dynamic reorganization of multivesicular bodies and exosome 
            production impacted by sonoporation.
  JOURNAL   Sci Rep. 2024 Nov 9;14(1):27432. doi: 10.1038/s41598-024-79042-6.
  PUBMED    39521850
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-11-9"; volume: "14(1):27432. doi"; pages: " 
            10.1038/s41598-024-79042-6"
COMMENT     Sequence Label: pKT2/CAGXSP/GFPCD63
FEATURES             Location/Qualifiers
     source          1..8395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(319..336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(490..1078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(570..589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1221..2030)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1944..1963
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2022..2041
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     polyA_signal    2535..2656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2719..2738)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2910..3289
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3602..4618
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4542..4564
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4626..4645
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4845..4854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4851..4853,4854..4856,4857..5567)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4851..4853,4854..4856,4857..5567)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4896..4917)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5157..5176)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5504..5525
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5643..6359
                     /codon_start=1
                     /product="human CD63 tetraspanin"
                     /label=CD63
                     /note="enriched in intraluminal vesicles of late endosomes 
                     (multivesicular bodies) and in exosomes"
                     /translation="MAVEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQ
                     GATPGSLLPVVIIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAG
                     YVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKN
                     RVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIV
                     FACCLVKSIRSGYEVM"
     primer_bind     complement(6417..6434)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6423..6647
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6693..7121
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6780..6799)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6990..7011
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7130..7150)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7135..7464
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7315..7450
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7377..7396
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7475..8074
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7475..7494)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7971..7991
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8234..8355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        join(8386..8395,1..9)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cactaaaggg agaccaagtt aaacaattta aaggcaatgc taccaaatac taattgagtg
       61 tatgtaaact tctgacccac tgggaatgtg atgaaagaaa taaaagctga aatgaatcat
      121 tctctctact attattctga tatttcacat tcttaaaata aagtggtgat cctaactgac
      181 ctaagacagg gaatttttac taggattaaa tgtcaggaat tgtgaaaaag tgagtttaaa
      241 tgtatttggc taaggtgtat gtaaacttcc gacttcaact gtatagggat ctggtaccat
      301 ttaaatctgt tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      361 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      421 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      661 ttcgggaacc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      901 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1141 gattttggtc atgagattat caaaaaggat cttcacctag atcctttttg ccagtgttac
     1201 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     1261 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     1321 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     1381 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     1441 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     1501 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     1561 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     1621 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     1681 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc ggggatcgca
     1741 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     1801 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     1861 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt
     1921 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     1981 atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt
     2041 gtattactgt ttatgtaagc agacagtttt attgttcatg atgcagctgg atccagatcc
     2101 ctatacagtt gaagtcggaa gtttacatac acttaagttg gagtcattaa aactcgtttt
     2161 tcaactactc cacaaatttc ttgttaacaa acaatagttt tggcaagtca gttaggacat
     2221 ctactttgtg catgacacaa gtcatttttc caacaattgt ttacagacag attatttcac
     2281 ttataattca ctgtatcaca attccagtgg gtcagaagtt tacatacact aagttgactg
     2341 tgcctttaaa cagcttggaa gctgcgcact aggcaagtta actaactcct ctgaatgtca
     2401 gtatttccat ctgtaagatg aacacagtgg ggctccaatt ccataccaca tttgtagagg
     2461 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     2521 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2581 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2641 tcatcaatgt atcttatcat gtctggccag ctatcgacgg atcgggagat ctcccgatcc
     2701 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     2761 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     2821 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     2881 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     2941 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     3001 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     3061 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     3121 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     3181 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3241 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
     3301 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     3361 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg gggggggccc
     3421 cggggggggg gggggggggg ggggggggcg gcgggggggg gcggggcggg ggggggggcg
     3481 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     3541 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     3601 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     3661 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     3721 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     3781 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     3841 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     3901 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     3961 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     4021 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     4081 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     4141 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     4201 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     4261 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     4321 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     4381 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     4441 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     4501 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     4561 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     4621 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgagga
     4681 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     4741 ggctcacaaa taccactgag atctgcatga gctagcgttt aaacttaagc ttggtaccga
     4801 gctcggatcc actagtccag tgtggtggaa ttctgcagat agccgccacc atggtgagca
     4861 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4921 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4981 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     5041 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     5101 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5161 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5221 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5281 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5341 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5401 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5461 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5521 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggccggactc
     5581 agatctcgag ctcaagcttc gaattctgca gtcgacggta ccagagccca ggcccggcag
     5641 ccatggcggt ggaaggagga atgaaatgtg tgaagttctt gctctacgtc ctcctgctgg
     5701 ccttttgcgc ctgtgcagtg ggactgattg ccgtgggtgt cggggcacag cttgtcctga
     5761 gtcagaccat aatccagggg gctacccctg gctctctgtt gccagtggtc atcatcgcag
     5821 tgggtgtctt cctcttcctg gtggcttttg tgggctgctg cggggcctgc aaggagaact
     5881 attgtcttat gatcacgttt gccatctttc tgtctcttat catgttggtg gaggtggccg
     5941 cagccattgc tggctatgtg tttagagata aggtgatgtc agagtttaat aacaacttcc
     6001 ggcagcagat ggagaattac ccgaaaaata accacactgc ttcgatcctg gacaggatgc
     6061 aggcagattt taagtgctgt ggggctgcta actacacaga ttgggagaaa atcccttcca
     6121 tgtcgaagaa ccgagtcccc gactcctgct gcattaatgt tactgtgggc tgtgggatta
     6181 atttcaacga gaaggcgatc cataaggagg gctgtgtgga gaagattggg ggctggctga
     6241 ggaaaaatgt gctggtggta gctgcagcag cccttggaat tgcttttgtc gaggttttgg
     6301 gaattgtctt tgcctgctgc ctcgtgaaga gtatcagaag tggctacgag gtgatgtaga
     6361 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     6421 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6481 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6541 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6601 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6661 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     6721 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6781 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6841 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6901 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6961 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7021 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7081 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     7141 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7201 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7261 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7321 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7381 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     7441 tttggaggcc taggcttttg caaaaagctc ccgcatgacc gagtacaagc ccacggtgcg
     7501 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     7561 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     7621 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     7681 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7741 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7801 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7861 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7921 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7981 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     8041 cacctggtgc atgacccgca agcccggtgc ctgattcgaa atgaccgacc aagcgacgcc
     8101 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     8161 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     8221 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8281 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8341 catcaatgta tcttatcatg tctgtatacc gtcgatagcg atatcaatta accct
//
