LOCUS       sequence-461803-       19570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LbCas12a variant with D156R and E795L mutations and harboring same 
            NLS sequence as PEmax but codon-optimized for dicot species; for 
            Agrobacterium-mediated Arabidopsis transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19570)
  AUTHORS   Xin C, Qiao D, Wang J, Sun W, Cao Z, Lu Y, Jiang Y, Chai Y, Wang XC,
            Chen QJ
  TITLE     Enhanced editing efficiency in Arabidopsis with a LbCas12a variant 
            harboring D156R and E795L mutations.
  JOURNAL   aBIOTECH. 2024 Mar 26;5(2):117-126. doi: 10.1007/s42994-024-00144-w.
            eCollection 2024 Jun.
  PUBMED    38978783
REFERENCE   2  (bases 1 to 19570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s42994-024-00144-w"; journalName: "aBIOTECH"; date: 
            "2024-03-26- 26"; volume: "5"; issue: "2"; pages: "117-126"
COMMENT     Sequence Label: pBG-ttLbUV2
FEATURES             Location/Qualifiers
     source          1..19570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1126..1145
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1425..1677
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     promoter        2165..2269
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2270..2338,2339..3130)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2488..2507)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             4782..4802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8529..8549
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8559..8585
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     primer_bind     complement(9242..9258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9242..9258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9255..9277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9266..9282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9290..9296,9297..9314,9315..9320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9335..9356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    11602..11626
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12090..12884
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      14278..14364
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      14456..14483
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      14589..15177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15078..15097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15331..15348
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15363..15503
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15429..15448)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15589..15611
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15847..16041
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16107..17180)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17609..18238)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag ctattcgcta ccttaggacc gttatagtta cgagcttaca attgattgac
      301 aactaagctg gcacaactat atttccaaca tcactagcta ccatcaaaag attgacttct
      361 catcttactc gattgaaacc aaattaacat agggttttta tttaaataaa agtttaacct
      421 tctttttaaa aaattgttca tagtgtcatg tcagaacaag agctacaaat cacacatagc
      481 atgcataagc ggagctatga tgagtggtat tgttttgttc gtcacttgtc actcttttcc
      541 aacacataat cccgacaaca acgtaagagc atctctctct ctccacacac actcatgcat
      601 gcatgcattc ttacacgtga ttgccatgca aatctccttt ctcacctata aatacaaacc
      661 aacccttcac tacactcttc actcaaacca aaacaagaaa acatacacaa atagcaaaac
      721 ggtaccaaca atggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
      781 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      841 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
      901 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
      961 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     1021 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     1081 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     1141 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     1201 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     1261 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     1321 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     1381 gggccgccac tccaccggcg gcatggacga gctgtacaag tgagcttgga ctcccatgtt
     1441 ggcaaaggca accaaacaaa caatgaatga tccgctcctg catatggggc ggtttgagta
     1501 tttcaactgc catttgggct gaattgaaga catgctcctg tcagaaattc cgtgatctta
     1561 ctcaatattc agtaatctcg gccaatatcc taaatgtgcg tggctttatc tgtctttgta
     1621 ttgtttcatc aattcatgta acgtttgctt ttcttatgaa ttttcaaata aattatcgta
     1681 ttgagcctag gaagcttcga cttgccttcc gcacaataca tcatttcttc ttagcttttt
     1741 ttcttcttct tcgttcatac agtttttttt tgtttatcag cttacatttt cttgaaccgt
     1801 agctttcgtt ttcttctttt taactttcca ttcggagttt ttgtatcttg tttcatagtt
     1861 tgtcccagga ttagaatgat taggcatcga accttcaaga atttgattga ataaaacatc
     1921 ttcattctta agatatgaag ataatcttca aaaggcccct gggaatctga aagaagagaa
     1981 gcaggcccat ttatatggga aagaacaata gtatttctta tataggccca tttaagttga
     2041 aaacaatctt caaaagtccc acatcgctta gataagaaaa cgaagctgag tttatataca
     2101 gctagagtcg aagtagtgat tgtaatttct actaagtgta gatagagacc ttcggggaaa
     2161 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2221 gggacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2281 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2341 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2401 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2461 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2521 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2581 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2641 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2701 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2761 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2821 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2881 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2941 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggctctc gcggtatcat
     3001 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca ccacggggag
     3061 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3121 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3181 tttttaattt aaaaggatct aggtgaagat cctttttgat aatcccatga ccaaaatccc
     3241 ttaacgtgag ttttcgttcc actgagcgtc aggtctcagc cggcatggtc ccagcctcct
     3301 cgctggcgcc ggctgggcaa catgcttcgg catggcgaat gggacttttt tttgcaaaat
     3361 tttccagatc gatttcttct tcctctgttc ttcggcgttc aatttctggg gttttctctt
     3421 cgttttctgt aactgaaacc taaaatttga cctaaaaaaa atctcaaata atatgattca
     3481 gtggttttgt acttttcagt tagttgagtt ttgcagttcc gatgagataa accaatacta
     3541 gtagcttgat aaactatcgc caccaattta tttttgttgc gatattgtta ctttcctcag
     3601 tatgcagctt tgaaaagacc aaccctctta tcctttaaca atgaacaggt ttttagaggt
     3661 agcttgatga ttcctgcaca tgtgatcttg gcttcaggct taattttcca ggtaaagcat
     3721 tatgagatac tcttatatct cttacatact tttgagataa tgcacaagaa cttcataact
     3781 atatgcttta gtttctgcat ttgacactgc caaattcatt aatctctaat atctttgttg
     3841 ttgatctttg gtagacatgg gtactagaaa aagcaaacta caccaaggta aaatactttt
     3901 gtacaaacat aaactcgtta tcacggaaca tcaatggagt gtatatctaa cggagtgtag
     3961 aaacatttga ttattgcagg aagctatctc aggatattat cggtttatat ggaatctctt
     4021 ctacgcagag tatctgttat tccccttcct ctagctttca atttcatggt gaggatatgc
     4081 agttttcttt gtatatcatt cttcttcttc tttgtagctt ggagtcaaaa tcggttcctt
     4141 catgtacata catcaaggat atgtccttct gaatttttat atcttgcaat aaaaatgctt
     4201 gtaccaattg aaacaccagc tttttgagtt ctatgatcac tgacttggtt ctaaccaaaa
     4261 aaaaaaaaat gtttaattta catatctaaa agtaggttta gggaaaccta aacagtaaaa
     4321 tatttgtata ttattcgaat ttcactcatc ataaaaactt aaattgcacc ataaaatttt
     4381 gttttactat taatgatgta atttgtgtaa cttaagataa aaataatatt ccgtaagtta
     4441 accggctaaa accacgtata aaccagggaa cctgttaaac cggttcttta ctggataaag
     4501 aaatgaaagc ccatgtagac agctccatta gagcccaaac cctaaatttc tcatctatat
     4561 aaaaggagtg acattagggt ttttgttcgt cctcttaaag ctactcgttt tctctgccgt
     4621 ctctctcatt cgcgcgacgc aaacgatctt caggtgatct tctttctcca aatcctctct
     4681 cataactctg atttcgtact tgtgtatttg tgctcacgct ctgtttctct caccacagcc
     4741 tctagatgaa gcgtaccgct gatggcagcg agttcgagag ccctaagaag aagcgtaagg
     4801 tgtcaaagct cgagaagttc accaactgct actccctgag caagaccctg aggttcaagg
     4861 ccatcccagt cggcaagacc caggagaaca tcgacaacaa gaggctgctc gtggaggacg
     4921 agaagcgtgc cgaggactac aagggcgtga agaagctgct cgacaggtac tacctgtcat
     4981 tcatcaacga cgtcctgcac tcaatcaagc tgaagaacct gaacaactac atctcactgt
     5041 tccgtaagaa gaccaggacc gagaaggaga acaaggagct ggagaacctg gagatcaacc
     5101 tgcgtaagga gatcgccaag gctttcaagg gcaacgaggg ctacaagtca ctgttcaaga
     5161 aggacatcat cgagacaatc ctgccagagt tcctcgacga caaggacgag atcgccctgg
     5221 tcaactcctt caacggcttc accaccgcct tcactggctt cttcaggaac agagagaaca
     5281 tgttctctga ggaggccaag tccacctcta tcgccttcag gtgcatcaac gagaacctca
     5341 cccgttacat ctccaacatg gacatcttcg agaaggtgga cgccatcttc gacaagcacg
     5401 aggtgcagga gatcaaggag aagatcctca actccgacta cgacgtggag gacttcttcg
     5461 agggtgagtt cttcaacttc gtgctcactc aggagggtat cgacgtgtac aacgctatca
     5521 tcggtggctt cgtgactgag tccggtgaga agatcaaggg cctgaacgag tacatcaacc
     5581 tgtacaacca gaagaccaag cagaagctgc caaagttcaa gcctctgtac aagcaggtcc
     5641 tgtcagacag agagtcactg tcattctacg gcgagggcta cacttccgac gaggaggtcc
     5701 tggaggtgtt caggaacacc ctcaacaaga actccgagat cttctctagc atcaagaagc
     5761 tcgagaagct gttcaagaac ttcgacgagt actctagcgc tggcatcttc gtgaagaacg
     5821 gacctgccat ctccaccatc tccaaggaca tcttcggcga gtggaacgtg atcagagaca
     5881 agtggaacgc cgagtacgac gatattcacc tcaagaagaa ggccgtcgtc accgagaagt
     5941 acgaggacga cagacgtaag agcttcaaga agatcggctc cttcagcctg gagcagctgc
     6001 aggagtacgc tgacgctgac ctgagcgtcg tcgagaagct gaaggagatc atcatccaga
     6061 aggtcgatga gatctacaag gtctacggct ctagcgagaa gctgttcgac gctgacttcg
     6121 tgctggagaa gtcactgaag aagaacgacg ctgtcgtcgc tatcatgaag gacctgctcg
     6181 acagcgtgaa gtcattcgag aactacatca aggctttctt cggcgagggc aaggagacta
     6241 acagagacga gtcattctac ggcgacttcg tcctcgctta cgacatcctg ctcaaggtgg
     6301 accacatcta cgacgccatc agaaactacg tgacacagaa gccctacagc aaggacaagt
     6361 tcaagctcta cttccagaac ccacagttca tgggcggctg ggacaaggac aaggagactg
     6421 actacagggc caccatcctg aggtacggtt caaagtacta cctggccatc atggacaaga
     6481 agtacgccaa gtgcctccag aagatcgaca aggacgacgt caacggcaac tacgagaaga
     6541 tcaactacaa gctgctgccc ggtcccaaca agatgctccc aaaggtgttc ttcagcaaga
     6601 agtggatggc ctactacaac ccaagcgagg acatccagaa gatctacaag aacggcacct
     6661 tcaagaaggg cgacatgttc aacctgaacg actgccacaa gctgatcgac ttcttcaagg
     6721 actcaatcag cagataccca aagtggtcta acgcctacga cttcaacttc tccgagactg
     6781 agaagtacaa ggacatcgcc ggcttctacc gtgaggtgga ggagcagggc tacaaggtgt
     6841 cattcgagtc cgccagcaag aaggaggtgg acaagctcgt ggaggagggc aagctctaca
     6901 tgttccagat ctacaacaag gacttcagcg acaagagcca cggcacacct aacctccaca
     6961 ctatgtactt caagctgctg ttcgacgaga acaaccacgg ccagatcagg ctgtcaggtg
     7021 gtgctgagct gttcatgcgt agagccagcc tcaagaagga ggagctggtc gtgcaccctg
     7081 ctaactcacc aatcgccaac aagaaccctg acaaccctaa gaagactacc acactgagct
     7141 acgacgtgta caaggacaag aggttctccg aggaccagta cctgctgcac atccctatcg
     7201 ctatcaacaa gtgcccaaag aacatcttca agatcaacac agaggtgaga gtgctgctca
     7261 agcacgacga caacccttac gtcatcggca tcgacagagg cgagaggaac ctgctgtaca
     7321 tcgtcgtcgt ggacggcaag ggcaacatcg tcgagcagta ctccctgaac gagatcatca
     7381 acaacttcaa cggcatcaga atcaagaccg actaccactc cctgctcgac aagaaggaga
     7441 aggagcgttt cgaggctaga cagaactgga catccatcga gaacatcaag gagctgaagg
     7501 ctggttacat ctctcaggtc gtgcacaaga tctgcgagct ggtggagaag tacgacgctg
     7561 tgatcgctct ggaggacctc aactccggct tcaagaactc cagagtgaag gtggagaagc
     7621 aggtctacca gaagttcgag aagatgctga tcgacaagct caactacatg gtcgataaga
     7681 agtccaaccc atgcgccact ggtggtgctc tcaagggtta ccagatcaca aacaagttcg
     7741 agtccttcaa gtccatgtcc actcagaacg gcttcatctt ctacatccct gcttggctga
     7801 cctctaagat cgatccctca accggtttcg tcaacctgct caagaccaag tacacctcca
     7861 tcgccgactc caagaagttc atctctagct tcgacagaat catgtatgtc ccagaggagg
     7921 acctgttcga gttcgccctc gactacaaga acttctccag aaccgacgcc gactacatca
     7981 agaagtggaa gctctactcc tacggcaaca gaatcaggat cttcagaaac cctaagaaga
     8041 acaacgtctt cgactgggag gaggtgtgcc tgaccagcgc ctacaaggag ctgttcaaca
     8101 agtacggcat caactaccag cagggtgaca tcagagccct gctgtgcgag cagtctgaca
     8161 aggctttcta ctctagcttc atggctctga tgagcctgat gctccagatg agaaactcaa
     8221 tcacaggcag aaccgacgtg gacttcctga tctcacccgt gaagaactct gacggcatct
     8281 tctacgacag caggaactac gaggctcagg agaacgctat cctgccaaag aacgctgatg
     8341 ctaacggtgc ttacaacatc gctaggaagg tcctgtgggc tatcggccag ttcaagaagg
     8401 ctgaggatga gaagctggat aaggtcaaga tcgctatcag caacaaggag tggctggagt
     8461 acgctcagac atctgtgaag cactctggtg gatctaagag gactgccgac ggatctgagt
     8521 tcgagtcacc caagaagaag cgtaaggtcg gatctggacc tgctgctaag agagtcaagc
     8581 tcgactgagc tcagagcttt cgttcgtatc atcggtttcg acaacgttcg tcaagttcaa
     8641 tgcatcagtt tcattgcgca cacaccagaa tcctactgag tttgagtatt atggcattgg
     8701 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt
     8761 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc
     8821 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt
     8881 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc
     8941 tctaatgacc gaagttaata tgaggagtaa aacacttgta gttgtaccat tatgcttatt
     9001 cactaggcaa caaatatatt ttcagaccta gaaaagctgc aaatgttact gaatacaagt
     9061 atgtcctctt gtgttttaga catttatgaa ctttccttta tgtaattttc cagaatcctt
     9121 gtcagattct aatcattgct ttataattat agttatactc atggatttgt agttgagtat
     9181 gaaaatattt tttaatgcat tttatgactt gccaattgat tgacaacgaa ttcgtaatca
     9241 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9301 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9361 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9421 tcggccaacg cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt
     9481 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     9541 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     9601 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     9661 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     9721 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     9781 gcaagtggat tgatgtgatt ggcagacata ctgtcccaca aatgaagatg gaatctgtaa
     9841 aagaaaacgc gtgaaataat gcgtctgaca aaggttaggt cggctgcctt taatcaatac
     9901 caaagtggtc cctaccacga tggaaaaact gtgcagtcgg tttggctttt tctgacgaac
     9961 aaataagatt cgtggccgac aggtgggggt ccaccatgtg aaggcatctt cagactccaa
    10021 taatggagca atgacgtaag ggcttacgaa ataagtaagg gtagtttggg aaatgtccac
    10081 tcacccgtca gtctataaat acttagcccc tccctcattg ttaagggagc aaaatctcag
    10141 agagatagtc ctagagagag aaagagagca agtagcctag aagtagtcaa ggcggcgaag
    10201 tattcaggca cgtggccagg aagaagaaaa gccaagacga cgaaaacagg taagagctaa
    10261 gcctagatga gccccgagag gaggccagcg gacattagga gggcgactga ggcggatatg
    10321 ccagccgtct gcaccatcgt caatcactac atcgaaacct ccacggtgaa cttcaggaca
    10381 gagccgcagg agccccagga gtggactgac gatctcgtcc gcctgaggga gcgctacccg
    10441 tggctcgtcg ctgaggttga cggcgaggtg gctgggatcg cttacgctgg cccctggaag
    10501 gcgcgcaatg cttacgattg gaccgccgag agcacggttt acgtgtcgcc aaggcaccag
    10561 aggaccggcc tcgggtcaac actgtacact catctcctga agtccctgga ggctcagggc
    10621 ttcaagagcg tggtcgccgt tattggcctg ccaaacgacc cttctgtgcg gatgcacgag
    10681 gccctcggct acgctccacg cgggatgctg agggctgctg gcttcaagca cgggaattgg
    10741 catgacgtgg gcttctggca gctcgatttc tcgctgccgg tcccgccgag gccggtgctc
    10801 cccgtcacgg agatcctgaa cttcgacctc ctgaagctgg ccggcgatgt cgagtctaac
    10861 ccagggccta tgatcgaggt taagccgatt aatgcggagg acacctacga tctccgccac
    10921 agggtcctgc gcccaaatca gcctatcgag gcgtgcatgt tcgagtctga cctcacgcgc
    10981 tcagcgttcc atctgggcgg gttctacggc gggaagctca tttcagtggc gtccttccac
    11041 caggctgagc atagcgagct gcagggcaag aagcagtacc agctcagggg cgttgctaca
    11101 ctggagggct accgcgagca gaaggctggc tccagcctcg tcaagcacgc tgaggagatc
    11161 ctgcggaagc gcggcgcgga tatgatttgg tgcaacgctc ggacctcggc ctctggctac
    11221 taccgcaagc tcggcttctc ggagcagggg gaggtcttcg acaccccccc agttggcccc
    11281 catattctga tgtacaagcg gattacttag ctcgacggcc atgctagagt ccgcaaaaat
    11341 caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa tgtgtgagta
    11401 gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg agcatataag
    11461 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
    11521 aaaaccaaaa tccagtgacc tagtaattca gtacattaaa aacgtccgca atgtgttatt
    11581 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
    11641 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
    11701 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
    11761 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
    11821 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg ctggcaaaca
    11881 gctattatgg gtattatggg tctagtgggt ttcaaaatcg gctccgtcga tactatgtta
    11941 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
    12001 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
    12061 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
    12121 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
    12181 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
    12241 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
    12301 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
    12361 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
    12421 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
    12481 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
    12541 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
    12601 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
    12661 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
    12721 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
    12781 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
    12841 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta atctcgggga
    12901 cgtctaacta ctaagcgaga gtagggaact gccaggcact gagagatccc ctcataattt
    12961 ccccaaagcg taaccatgtg tgaataaatt ttgagctagt agggttgcag ccacgagtaa
    13021 gtcttccctt gttattgtgt agccagaatg ccgcaaaact tccatgccta agcgaactgt
    13081 tgagagtacg tttcgatttc tgactgtgtt agcctggaag tgcttgtccc aaccttgttt
    13141 ctgagcatga acgcccgcaa gccaacatgt tagttgaagc atcagggcga ttagcagcat
    13201 gatatcaaaa cgctctgagc tgctcgttcg gctatggcgt aggcctagtc cgtaggcagg
    13261 acttttcaag tctcggaagg tttcttcaat ctgcattcgc ttcgaataga tattaacaag
    13321 ttgtttgggt gttcgaattt caacaggtaa gttagttgct agaatccatg gctcctttgc
    13381 cgacgctgag tagattttag gtgacgggtg gtgacaatga gtccgtgtcg agcgctgatt
    13441 ttttcggcct ttagagcgag atttatacaa tagaatttgg catgagattg gattgctttt
    13501 agtcagcctc ttatagccta aagtctttga gtgactagat gacatatcat gtaagttgct
    13561 gataggtttc cagttttccg ctcctaggtc tgcatattgt acttttcctc ttactcgact
    13621 taaccagtac caacccagct tctcaacgga tttataccat ggcactttaa agccagcatc
    13681 actgacaatg agcggtgtgg tgttactcgg tagaatgctc gcaaggtcgg ctagaaattg
    13741 gtcatgagct ttctttgaac attgctctga aagcgggaac gctttctcat aaagagtaac
    13801 agaacgaccg tgtagtgcga ctgaagctcg caataccata agccgttttt gctcacggat
    13861 atcagaccag tcaacaagta caatgggcat cgtattgccc gaacagataa agctagcatg
    13921 ccaacggtat acagcgagtc gctctttgtg gaggtgacga ttacctaaca atcggtcgat
    13981 tcgtttgatg ttatgttttg ttctcgcttt ggttggcagg ttacggccaa gttcggtaag
    14041 agtgagagtt ttacagtcaa gtaaggcgtg gcaagccaac gttaagctgt tgagtcgttt
    14101 taagtgtaat tcggggcaga attggtaaag agagtcgtgt aaaatatcga gttcgcacat
    14161 tttgttgtct gattattgat ttttggcgaa accatttgat catatgacaa gatgtgtatc
    14221 taccttaact taatgatttt gataaaaatc attaggggat tcatcagtgc caggcatcaa
    14281 ataaaacgaa aggctcagtc ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg
    14341 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg
    14401 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaac taagcagaag
    14461 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttcctgttag atgcatgacc
    14521 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    14581 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    14641 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    14701 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
    14761 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    14821 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    14881 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    14941 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    15001 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    15061 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    15121 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    15181 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    15241 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    15301 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    15361 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
    15421 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
    15481 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
    15541 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
    15601 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
    15661 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
    15721 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
    15781 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
    15841 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
    15901 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
    15961 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
    16021 ccaatgtacg tgctatccac aggaaacaga ccttttcgac ctttttcccc tgctagggca
    16081 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
    16141 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
    16201 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
    16261 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
    16321 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
    16381 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
    16441 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
    16501 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
    16561 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
    16621 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
    16681 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
    16741 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
    16801 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
    16861 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
    16921 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
    16981 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
    17041 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
    17101 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
    17161 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
    17221 gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
    17281 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
    17341 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
    17401 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
    17461 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
    17521 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
    17581 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
    17641 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    17701 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    17761 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    17821 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    17881 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    17941 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    18001 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    18061 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    18121 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    18181 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    18241 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    18301 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    18361 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    18421 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    18481 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    18541 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    18601 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    18661 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    18721 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    18781 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    18841 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    18901 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    18961 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    19021 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    19081 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    19141 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    19201 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    19261 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    19321 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    19381 aagccgactg cactatagca gcggaggggt tggatcaaag tacccataat acccataata
    19441 gctgtttgcc agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg
    19501 gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct
    19561 tttctcttag
//
