LOCUS       sequence-461830-        9624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express split-Cdc42 fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   He Y, Faulkner BM, Weatherford RS, Hyun E, Stains CI
  TITLE     Split-Small GTPase Reassembly as a Method to Control Cellular 
            Signaling with User-Defined Inputs.
  JOURNAL   ACS Chem Biol. 2025 Aug 26. doi: 10.1021/acschembio.5c00083.
  PUBMED    40856289
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2025 Aug 26. doi: 10.1021/acschembio.5c00083."
COMMENT     Sequence Label: 
            pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(Q61L)-GAI1-92-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..9624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1091..1093,1094..1096,1097..1807)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(3013..3587,3588..3590,3591..3599)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3180..3197)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3407..3426
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4235..4252
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(4523..4525,4526..5236)
                     /codon_start=1
                     /product="monomeric optimized variant of ECFP (Kremers et 
                     al., 2006)"
                     /label=SCFP3A
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4523..4525,4526..5236)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    5328..5449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5339..5358)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5393..5412
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5635..6090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5722..5741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5932..5953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6207..6564
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6207..6398
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6230..6250)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6415..6550
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6615..7409
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6669..6688)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7279..7298
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7473..7521
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7562..7581)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7681..7703
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7741..7759)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7827..7931
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7932..8000,8001..8792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8150..8169)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8963..9551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9452..9471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1141 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1201 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1261 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga
     1321 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1381 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1441 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1501 cctggggcac aagctggagt acaacgccat cagcgacaac gtctatatca ccgccgacaa
     1561 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt
     1621 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1681 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga
     1741 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1801 gtacaagggc ggtggctcat ctggcggagg tatggctgcg agcgatgaag ttaatcttat
     1861 tgagagcaga acagtggttc ctctcaatac atgggtttta atatccaact tcaaagtagc
     1921 ctacaatatc cttcgtcgcc ctgatggaac ctttaaccga cacttagctg agtatctaga
     1981 ccgtaaagtc actgcaaacg ccaatccggt tgatggggtt ttctcgttcg atgtcttgat
     2041 tgatcgcagg atcaatcttc taagcagagt ctatagacca gcttatgcag atcaagagca
     2101 acctcctagt attttagatc tcgagaagcc tgttgatggc gacattgtcc ctgttatatt
     2161 gttcttccat ggaggtagct ttgctcattc ttctgcaaac agtgccatct acgatactct
     2221 ttgtcgcagg cttgttggtt tgtgcaagtg tgttgttgtc tctgtgaatt atcggcgtgc
     2281 accagagaat ccataccctt gtgcttatga tgatggttgg attgctctta attgggttaa
     2341 ctcgagatct tggcttaaat ccaagaaaga ctcaaaggtc catattttct tggctggtga
     2401 tagctctgga ggtaacatcg cgcataatgt ggctttaaga gcgggtgaat cgggaatcga
     2461 tgttttgggg aacattctgc tgaatcctat gtttggtggg aatgagagaa cggagtctga
     2521 gaaaagtttg gatgggaaat actttgtgac ggttagagac cgcgattggt actggaaagc
     2581 gtttttaccc gagggagaag atagagagca tccagcgtgt aatccgttta gcccgagagg
     2641 gaaaagctta gaaggagtga gtttccccaa gagtcttgtg gttgtcgcgg gtttggattt
     2701 gattagagat tggcagttgg catacgcgga agggctcaag aaagcgggtc aagaggttaa
     2761 gcttatgcat ttagagaaag caactgttgg gttttacctc ttgcctaata acaatcattt
     2821 ccataatgtt atggatgaga tttcggcgtt tgtaaacgcg gaatgtcaga tctcgtacgc
     2881 gtcccggggc ggtggctcat ctggcggagg tgagctccag acaattaagt gtgttgttgt
     2941 gggcgatggt gcttaagaat tcacgcgtcg agcatgcatc tagggcggcc aattccgccc
     3001 ctctcccccc cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     3061 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     3121 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     3181 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     3241 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     3301 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     3361 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     3421 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     3481 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     3541 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc
     3601 gggatcctct agaatggggg ttggtaaaac atgtctcctg atatcctaca caacaaacaa
     3661 atttccatcg gagtatgtac cgactgtttt tgacaactat gcagtcacag ttatgattgg
     3721 tggagaacca tatactcttg gactttttga tactgcaggg ctagaggatt atgacagatt
     3781 acgaccgctg agttatccac aaacagatgt atttctagtc tgtttttcag tggtctctcc
     3841 atcttcattt gaaaacgtga aagaaaagtg ggtgcctgag ataactcacc actgtccaaa
     3901 gactcctttc ttgcttgttg ggactcaaat tgatctcaga gatgacccct ctactattga
     3961 gaaacttgcc aagaacaaac agaagcctat cactccagag actgctgaaa agctggcccg
     4021 tgacctgaag gctgtcaagt atgtggagtg ttctgcactt acacagagag gtctgaagaa
     4081 tgtgtttgat gaggctatcc tagctgccct cgagcctccg gaaactcaac ccggggatcc
     4141 caattgggag ctcgtgtaca cggcgcgcct gcagggaggt ggctcatctg gcggaggtca
     4201 gatctcgtac gcgtcccggg gcatgaagag agatcatcat catcatcatc atcaagataa
     4261 gaagactatg atgatgaatg aagaagacga cggtaacggc atggatgagc ttctagctgt
     4321 tcttggttac aaggttaggt catccgaaat ggctgatgtt gctcagaaac tcgagcagct
     4381 tgaagttatg atgtctaatg ttcaagaaga cgatctttct caactcgcta ctgagactgt
     4441 tcactataat ccggcggagc tttacacgtg gcttgattct atgctcaccg accttaatgg
     4501 cggtggctca tctggcggag gtgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4561 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4621 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4681 gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg
     4741 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4801 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4861 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4921 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
     4981 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     5041 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5101 gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga
     5161 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5221 ggacgagctg tacaagaaaa aaaagaagaa aaagagcaag accaaatgcg tgattatgta
     5281 agcggccgct tccctttagt gagggttaat gcttcgagca gacatgataa gatacattga
     5341 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     5401 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     5461 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta
     5521 aaacctctac aaatgtggta aaatccgata aggatcgatc cgggctggcg taatagcgaa
     5581 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
     5641 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5701 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5761 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5821 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5881 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5941 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6001 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6061 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     6121 gtgcggtatt tcacaccgca tacgcggatc tgcgcagcac catggcctga aataacctct
     6181 gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt
     6241 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6301 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6361 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6421 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6481 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6541 tttggaggcc taggcttttg caaaaagctt gattcttctg acacaacagt ctcgaactta
     6601 aggctagagc caccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6661 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     6721 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6781 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6841 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     6901 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     6961 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     7021 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     7081 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     7141 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     7201 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     7261 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     7321 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     7381 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     7441 agcgacgccc aacctgccat cacgatggcc gcaataaaat atctttattt tcattacatc
     7501 tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat ccgcgtatgg tgcactctca
     7561 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     7621 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     7681 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     7741 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7801 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7861 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7921 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7981 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8041 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8101 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8161 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8221 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8281 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8341 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8401 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8461 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8521 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8581 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8641 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8701 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8761 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8821 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8881 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8941 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9001 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9061 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9121 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9181 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9241 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9301 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9361 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9421 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9481 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9541 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9601 ttgctcacat ggctcgacag atct
//
