LOCUS       sequence-461833-       10803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one 
            grazoprevir-inducible mGreenLantern expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Blanch-Asensio A, Ploessl DS, Wang NB, Mummery CL, Galloway KE, 
            Davis RP
  TITLE     STRAIGHT-IN Dual: a platform for dual, single-copy integrations of 
            DNA payloads and gene circuits into human induced pluripotent stem 
            cells.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 
            10.1101/2024.10.17.616637.
  PUBMED    39464154
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 
            10.1101/2024.10.17.616637."
COMMENT     Sequence Label: Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor
FEATURES             Location/Qualifiers
     source          1..10803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        275..471
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      322..457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        558..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          789..1727
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1684..1704
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1744..1753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1756..1793
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    1808..1855
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      2353..2362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2359..2361,2362..2364,2365..3078)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(2404..2425)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    3080..3668
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3133..3153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3551..3562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     enhancer        3725..4104
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4106..4379
                     /label=chicken beta-actin promoter
     intron          4380..5396
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5320..5342
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5404..5423
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6014..6079
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6086..6106
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6956..6982
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7208..7579
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     polyA_signal    7589..7813
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7861..7894
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(7902..7918)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7932..7948)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7941..7963)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8161..8616
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8248..8267)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8458..8479
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8633..8652)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8752..8774
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8812..8830)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8898..9002
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9003..9071,9072..9863)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9221..9240)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10034..10622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10523..10542
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10776..10793
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggactccg tcgtcaggat catccggatc
     1801 cggatccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcatgat
     1861 attcggcaag caggcatcga ctagttaatt aagcggacga ttgagtaaca ctgtatctag
     1921 tctacctaca gggctcggcg tagccgatgt cgcgcggata gcgtcaacct cttcggcgta
     1981 gccgatgtcg cgcaatcagt tctcccgagg tcggcgtagc cgatgtcgcg cagagccgtg
     2041 cttccaatac ggcgtagccg atgtcgcgcc gggtttcgta acaatcgcat gaggattcgc
     2101 aacgccttcg gcgtagccga tgtcgcgctc ccgtctcagt aaaggtcggc gtagccgatg
     2161 tcgcgcaatc ggactgcctt cgtacggcgt agccgatgtc gcgcgtatca gtcgcctcgg
     2221 aacggcgtag ccgatgtcgc gcattcgtaa gaggctcact ctcccttaca cggagtggat
     2281 aggatcctct agagggtata taatgggggc cactagtcta ctaccagagc tcatcgctag
     2341 cgctaccgga tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2401 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtccg cggcgagggc
     2461 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2521 cccgtgccct ggcccaccct cgtgaccacc ttaggctacg gcgtggcctg cttcgcccgc
     2581 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2641 caggagcgca ccatctcttt caaggacgac ggtacctaca agacccgcgc cgaggtgaag
     2701 ttcgagggcg acaccctggt gaaccgcatc gtgctgaagg gcatcgactt caaggaggac
     2761 ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaaggt ctatatcacg
     2821 gccgacaagc agaagaacgg catcaaggct aacttcaaga cccgccacaa cgttgaggac
     2881 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2941 ctgctgcccg acaaccacta cctgagccat cagtccaaac tgagcaaaga ccccaacgag
     3001 aagcgcgatc acatggtcct gaaggagagg gtgaccgccg ccgggattac acatgacatg
     3061 gacgagctgt acaagtaaga atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3121 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3181 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3241 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3301 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3361 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3421 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     3481 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3541 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3601 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3661 ctccccgccc tttgggccgc ctccccgcgc tagtgctggg gatgcggtgg gctctatggg
     3721 tctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     3781 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3841 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3901 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3961 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     4021 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     4081 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     4141 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     4201 gcgatggggg cggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
     4261 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     4321 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     4381 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     4441 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     4501 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     4561 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     4621 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     4681 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     4741 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     4801 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     4861 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     4921 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     4981 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     5041 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     5101 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     5161 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     5221 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     5281 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     5341 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     5401 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc cgatcgcgcc
     5461 acctgccgcc accatgtcta gacccggaga gcgcccattc cagtgtcgga tttgcatgcg
     5521 gaacttttcg agaagacacg gcctggacag acatacccgt actcatacag gtgaaaaacc
     5581 ctttcagtgt cggatctgta tgcgaaattt ctccgaccac agcagcctga agagacatct
     5641 acgtacccac accggcagcc agaagccatt tcagtgtcgg atctgtatgc ggaacttctc
     5701 cgtgagacac aacctgacca gacatctacg tacgcacacc ggagagaagc cattccaatg
     5761 ccgaatatgc atgcgcaact tcagtgacca cagcaacctg agcagacacc taaaaaccca
     5821 caccggttcc cagaagccat ttcagtgtcg gatctgtatg cggaacttct cccagcgcag
     5881 cagcctggtg agacatctac gtacgcacac cggagagaag ccattccaat gccgaatatg
     5941 catgcgcaac ttcagtgaga gcggccacct gaagagacac ctgcgtacgc acctgagggg
     6001 atccacctgc agggactaca aagaccatga cggtgattat aaagatcatg acatcgatta
     6061 caaggatgac gatgacaaga tggcccccaa gaaaaagagg aaggtgggca ttcacggggt
     6121 gccgggtgga ctcgagggag gcggtggaag cggcggtacc gaggacgtgg tgtgctgcca
     6181 ctcaatctac ggcaagaaga agggtgatat cgacacctac cgatacatag gctcttccgg
     6241 gacaggctgc gtggtcatag tgggcaggat cgtcttgtcc ggatccggca ctagtgcgcc
     6301 catcacggcg tacgcccagc agacgagagg cctcctaggg tgtataatca ccagcctgac
     6361 tggccgggac aaaaaccaag tggagggtga ggtccagatc gtgtcaactg ctacccaaac
     6421 cttcctggca acgtgcatca atggggtatg ctgggcagtc taccacgggg ccggaacgag
     6481 gaccatcgca tcacccaagg gtcctgtcat ccagatgtat accaatgtgg accaagacct
     6541 tgtgggctgg cccgctcctc aaggttcccg ctcattgaca ccctgtacct gcggctcctc
     6601 ggacctttac ctggtcacga ggcacgccga tgtcattccc gtgcgccggc gaggtgatag
     6661 caggggtagc ctgctttcgc cccggcccat ttcctacttg aaaggctcct cggggggtcc
     6721 gctgttgtgc cccgcgggac acgccgtggg cctattcagg gccgcggtgt gcacccgtgg
     6781 agtggctaaa gcggtggact ttatccctgt ggagaaccta gagacaacca tgagatcccc
     6841 ggtgttcacg gacaactcct ctccaccagc agtcaccctg acgcacccaa tcaccaaaat
     6901 cgatagggag gttctctacc aggagttcga tgagatggaa gagtgctctc agcactatcc
     6961 ctacgatgtg cccgattacg ctggaggcgg tggaagcggc ggtaccgatg agtttcccac
     7021 catggtgttt ccttctgggc agatcagcca ggcctcggcc ttggccccgg cccctcccca
     7081 agtcctgccc caggctccag cccctgcccc tgctccagcc atggtatcag ctctggccca
     7141 ggccccagcc cctgtcccag tcctagcccc aggccctcct caggctgtgg ccccacctgc
     7201 ccccaagccc acccaggctg gggaaggaac gctgtcagag gccctgctgc agctgcagtt
     7261 tgatgatgaa gacctggggg ccttgcttgg caacagcaca gacccagctg tgttcacaga
     7321 cctggcatcc gtcgacaact ccgagtttca gcagctgctg aaccagggca tacctgtggc
     7381 cccccacaca actgagccca tgctgatgga gtaccctgag gctataactc gcctagtgac
     7441 aggggcccag aggccccccg acccagctcc tgctccactg ggggccccgg ggctccccaa
     7501 tggcctcctt tcaggagatg aagacttctc ctccattgcg gacatggact tctcagccct
     7561 gctgagtcag atcagctccc aattgtaact gtgccttcta gttgccagcc atctgttgtt
     7621 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7681 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7741 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     7801 gtgggctcta tggctagccg ctgcaagaac tcttcctcac ggatccgaag ttcctattcc
     7861 gaagttccta ttcttcaaat agtataggaa cttcagcgct cactggccgt cgttttacaa
     7921 agggcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7981 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8041 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     8101 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     8161 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8221 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8281 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8341 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     8401 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8461 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     8521 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     8581 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     8641 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     8701 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     8761 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     8821 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     8881 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     8941 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9001 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9061 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9121 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9181 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9241 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9301 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9361 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9421 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9481 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9541 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9601 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     9661 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     9721 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     9781 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     9841 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     9901 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9961 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10021 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10081 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10141 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10201 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10261 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10321 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10381 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10441 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10501 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10561 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10621 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    10681 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    10741 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    10801 aag
//
