LOCUS       sequence-46188-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human SIRT1 with flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   North BJ, Marshall BL, Borra MT, Denu JM, Verdin E
  TITLE     The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin 
            deacetylase.
  JOURNAL   Mol Cell. 2003 Feb . 11(2):437-44.
  PUBMED    12620231
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2003 Feb . 11(2):437-44."
COMMENT     Sequence Label: SIRT1 Flag
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt gcggccgcat ggcggacgag
      121 gcggcggcgg caggcgggga gcaagaggcc caggcgactg cggcggctgg ggaaggagac
      181 aatgggccgg gcctgcaggg cccatctcgg gagccaccgc tggccgacaa cttgtacgac
      241 gaagacgacg acgacgaggg cgaggaggag gaagaggcgg cggcggcggc gattgggtac
      301 cgagataacc ttctgttcgg tgatgaaatt atcactaatg gttttcattc ctgtgaaagt
      361 gatgaggagg atagagcctc acatgcaagc tctagtgact ggactccaag gccacggata
      421 ggtccatata cttttgttca gcaacatctt atgattggca cagatcctcg aacaattctt
      481 aaagatttat tgccggaaac aatacctcca cctgagttgg atgatatgac actgtggcag
      541 attgttatta atatcctttc agaaccacca aaaaggaaaa aaagaaaaga tattaataca
      601 attgaagatg ctgtgaaatt actgcaagag tgcaaaaaaa ttatagttct aactggagct
      661 ggggtgtctg tttcatgtgg aatacctgac ttcaggtcaa gggatggtat ttatgctcgc
      721 cttgctgtag acttcccaga tcttccagat cctcaagcga tgtttgatat tgaatatttc
      781 agaaaagatc caagaccatt cttcaagttt gcaaaggaaa tatatcctgg acaattccag
      841 ccatctctct gtcacaaatt catagccttg tcagataagg aaggaaacta cttcgcaact
      901 ataccc
//
