LOCUS       sequence-46189-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human SIRT1 with flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   North BJ, Marshall BL, Borra MT, Denu JM, Verdin E
  TITLE     The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin 
            deacetylase.
  JOURNAL   Mol Cell. 2003 Feb . 11(2):437-44.
  PUBMED    12620231
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2003 Feb . 11(2):437-44."
COMMENT     Sequence Label: SIRT1 Flag
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..27)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atacttgtca tcgtcgtcct tgtagtctcc tccgcggccg cttgatttgt ttgatggata
       61 gttcatgtct gttacttcct gtttcacaga tatagcttca ttaattgcct cttgatcatc
      121 tccatcagtc ccaaatccag ctcctccagc tctctctgga acatcaggct catcttctaa
      181 gccattgtag aattcttcaa tttcactttc atcctccatg ggttcttcta aacttggact
      241 ctggcatgtc ccactatcac tgttactgcc acaagaacta gaggataaga cgtcatcttc
      301 agagtctgaa tatacctcag cgccatggaa aatgtaacga tttggtggca aaaacagata
      361 ctgattacca tcaagccgcc tactaatctg ctcctttgcc actctattag gccagcattt
      421 tctcactgtt ccagccactg aagttctttc atttttctcc ccagtactag aaccaacatt
      481 cttcaaatcc ggattttcca tctgttcagc aatactttca acattcctag aagtttgtac
      541 ttcctgtggt ttttcttcca tacaaccttt tgattcagac acatctaaat catcattact
      601 cttagctgct tggtctaaaa gtgtgacaat cactgaagaa tctggtggtg aagttctttc
      661 tggtgaactt gagtcttctg aaacatgaag aggtgtgggt ggcaactctg acaaataagc
      721 caattctttt tgtgttcgtg gaggtttttc agtaatttct gaaagcttta cagggttaca
      781 gcaaagtttg gcatattcac cacctaacct atgacacaat tcattaatta tgacatcaca
      841 gtctccaaga agctct
//
