LOCUS       sequence-461903-        5689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0078m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP12286 - pAAV-AiE0078m-minBG-iCre-WPRE-HGHpA...
FEATURES             Location/Qualifiers
     source          1..5689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      730..739
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             742..762
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1816..2404
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1869..1889)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2287..2298)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2436..2912
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2635..2654)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2952..3092
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2952..3081
                     /label=AAV2 ITR
     rep_origin      3167..3622
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3254..3273)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3464..3485
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3639..3658)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3758..3780
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3818..3836)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3904..4008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4009..4077,4078..4869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4227..4246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5040..5628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5529..5548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtccctgg ccttcgagca catgctcaga tgatgctcca
      181 ccgtggcctg acccacatct tctagtggaa gcatggtcca gcaaagcctt tctgttctaa
      241 aggaaaggat ctgagttgtc acctcccagg tccgtggaag gctttttagc agtttggcag
      301 gtgcctgagg gccacacctc atctgcataa aatgtggcag attgcaaccg ccctcgtctt
      361 ttttattttt aaactggttt ttgaaacaga acatatataa aagctcagag aaagggaaag
      421 gagatagatg gccgagcttc catatccctt agtgccttta ttttcattct ttttccattt
      481 tcctaagtgg ctatttacca agacaaagat aacaaatctg ctaggaaaag gagtgggcag
      541 tgctacaaaa tgtttttttt tttttaaaga aagtcctatc ttataataga tcttcaccac
      601 gatgcctcat gatgtatgct caaatcagtt ttaattgaac tgtgtgtagt atgctcctgt
      661 tttggagctc gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt
      721 ctgggatccg ccaccatggt gcccaagaag aagaggaaag tctccaacct gctgactgtg
      781 caccaaaacc tgcctgccct ccctgtggat gccacctctg atgaagtcag gaagaacctg
      841 atggacatgt tcagggacag gcaggccttc tctgaacaca cctggaagat gctcctgtct
      901 gtgtgcagat cctgggctgc ctggtgcaag ctgaacaaca ggaaatggtt ccctgctgaa
      961 cctgaggatg tgagggacta cctcctgtac ctgcaagcca gaggcctggc tgtgaagacc
     1021 atccaacagc acctgggcca gctcaacatg ctgcacagga gatctggcct gcctcgccct
     1081 tctgactcca atgctgtgtc cctggtgatg aggagaatca gaaaggagaa tgtggatgct
     1141 ggggagagag ccaagcaggc cctggccttt gaacgcactg actttgacca agtcagatcc
     1201 ctgatggaga actctgacag atgccaggac atcaggaacc tggccttcct gggcattgcc
     1261 tacaacaccc tgctgcgcat tgccgaaatt gccagaatca gagtgaagga catctcccgc
     1321 accgatggtg ggagaatgct gatccacatt ggcaggacca agaccctggt gtccacagct
     1381 ggtgtggaga aggccctgtc cctgggggtt accaagctgg tggagagatg gatctctgtg
     1441 tctggtgtgg ctgatgaccc caacaactac ctgttctgcc gggtcagaaa gaatggtgtg
     1501 gctgcccctt ctgccacctc ccaactgtcc acccgggccc tggaagggat ctttgaggcc
     1561 acccaccgcc tgatctatgg tgccaaggat gactctgggc agagatacct ggcctggtct
     1621 ggccactctg ccagagtggg tgctgccagg gacatggcca gggctggtgt gtccatccct
     1681 gaaatcatgc aggctggtgg ctggaccaat gtgaacattg tgatgaacta catcagaaac
     1741 ctggactctg agactggggc catggtgagg ctgctcgagg atggggacta agaattcgat
     1801 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     1861 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     1921 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     1981 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2041 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2101 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2161 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2221 tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2281 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2341 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2401 ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc
     2461 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     2521 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     2581 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     2641 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     2701 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     2761 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     2821 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     2881 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac
     2941 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3001 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3061 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     3121 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     3181 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3241 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3301 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3361 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     3421 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3481 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
     3541 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3601 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     3661 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3721 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3781 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     3841 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3901 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3961 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4021 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4261 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4681 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4861 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4981 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5041 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5101 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5161 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5221 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5281 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5341 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5401 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5461 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5521 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5581 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5641 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
