LOCUS       sequence-461924-        4649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0121h is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP13788 - pAAV-AiE0121h-minBG-iCre(R297T)-BGH...
FEATURES             Location/Qualifiers
     source          1..4649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      585..594
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             597..617
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1683..1794
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1912..2052
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1912..2041
                     /label=AAV2 ITR
     rep_origin      2127..2582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2214..2233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2424..2445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2599..2618)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2718..2740
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2778..2796)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2864..2968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2969..3037,3038..3829)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3187..3206)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4000..4588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4489..4508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgaaaat gactctatct ggtttataat tagaagtgaa
      181 atgattttaa atggcttcca catggcttag ttttccaagc agttccagtt acatagaact
      241 tcatataaac agataatgag aggctagagt tggagagcac agttcctgct tccaaattgg
      301 cttgaagtgg ggacatctgc tctgcatgaa gcctgggtca ggctgcccct ctcatctgtg
      361 acctcctgtg gtcgggaggg acttgctctg gattatataa aggcttcaca agagtactgg
      421 aagcggggac ggaatcaact tgagcaaaaa acctaattgg cttgaatctt tccttgacca
      481 tcagtaatta ttttgagatt gatgagctcg ggctgggcat aaaagtcagg gcagagccat
      541 ctattgctta catttgcttc tgggatccag atctttcgaa gctagccacc atggtgccca
      601 agaagaagag gaaagtctcc aacctgctga ctgtgcacca aaacctgcct gccctccctg
      661 tggatgccac ctctgatgaa gtcaggaaga acctgatgga catgttcagg gacaggcagg
      721 ccttctctga acacacctgg aagatgctcc tgtctgtgtg cagatcctgg gctgcctggt
      781 gcaagctgaa caacaggaaa tggttccctg ctgaacctga ggatgtgagg gactacctcc
      841 tgtacctgca agccagaggc ctggctgtga agaccatcca acagcacctg ggccagctca
      901 acatgctgca caggagatct ggcctgcctc gcccttctga ctccaatgct gtgtccctgg
      961 tgatgaggag aatcagaaag gagaatgtgg atgctgggga gagagccaag caggccctgg
     1021 cctttgaacg cactgacttt gaccaagtca gatccctgat ggagaactct gacagatgcc
     1081 aggacatcag gaacctggcc ttcctgggca ttgcctacaa caccctgctg cgcattgccg
     1141 aaattgccag aatcagagtg aaggacatct cccgcaccga tggtgggaga atgctgatcc
     1201 acattggcag gaccaagacc ctggtgtcca cagctggtgt ggagaaggcc ctgtccctgg
     1261 gggttaccaa gctggtggag agatggatct ctgtgtctgg tgtggctgat gaccccaaca
     1321 actacctgtt ctgccgggtc agaaagaatg gtgtggctgc cccttctgcc acctcccaac
     1381 tgtccacccg ggccctggaa gggatctttg aggccaccca ccgcctgatc tatggtgcca
     1441 aggatgactc tgggcagaga tacctggcct ggtctggcca ctctgccaga gtgggtgctg
     1501 ccaccgacat ggccagggct ggtgtgtcca tccctgaaat catgcaggct ggtggctgga
     1561 ccaatgtgaa cattgtgatg aactacatca gaaacctgga ctctgagact ggggccatgg
     1621 tgaggctgct cgaagatggg gactgaggcg cgccgaattc aagcttctcg agagatcttc
     1681 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     1741 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     1801 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     1861 ttgggaagac aatagcaggc atgagatctc acgtgcggac cgagcggccg caggaacccc
     1921 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     1981 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     2041 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2101 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     2161 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     2221 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2281 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2341 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2401 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     2461 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     2521 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     2581 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     2641 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2701 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2761 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2821 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2881 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2941 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3001 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3061 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3121 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3181 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3241 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3301 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3361 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3421 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3481 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3541 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3601 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3661 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3721 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3781 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3841 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3901 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3961 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4021 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4081 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4141 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4201 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4261 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4321 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4381 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4441 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4501 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4561 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4621 ggccttttgc tggccttttg ctcacatgt
//
