LOCUS       sequence-46193-e        9713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pExG
FEATURES             Location/Qualifiers
     source          1..9713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(122..226)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(252..707)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(389..410)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     601..620
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     834..856
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     848..865
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     849..865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     872..891
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        872..890
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        1084..1387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1389..1666
                     /label=chicken beta-actin promoter
     intron          1668..2685
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2609..2631
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2693..2712
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2772..2781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2823..2844)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(3075..3108)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3228..4652
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVL
                     KGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYER
                     SEGRHHLFLYGMDELYK"
     CDS             4662..4691
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4698..4727
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4734..4763
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4894..5015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4931..4950)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4985..5004
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5229..5298
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        5374..5873
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5409..5432)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5806..5825
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5891..6916
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5891..6916
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    7679..7726
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(7774..7792)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7813..7829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7813..7829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7826..7848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7837..7853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7861..7867,7868..7885,7886..7891))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7906..7927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8044..8061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8215..8803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8295..8314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8974..9713,1..52,53..121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9597..9616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
       61 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
      121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      241 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
      301 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
      361 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
      421 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
      481 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
      541 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
      601 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
      661 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
      721 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      781 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      841 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg
      901 taccaagctt cccttaattg agaagttcct attccgaagt tcctattctt caaaaggtat
      961 aggaacttct gcactcctac agcctaaatg gcaaggactg ggaatatatc cagtgcagga
     1021 atctagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1081 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1141 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1201 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1261 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1321 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1381 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1441 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1501 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     1561 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1621 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggga gtcgctgcga
     1681 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     1741 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1801 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     1861 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     1921 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     1981 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     2041 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     2101 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     2161 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     2221 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     2281 gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
     2341 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     2401 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     2461 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     2521 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
     2581 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     2641 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     2701 ctggttattg tgctgtctca tcattttggc aaagaattga tttgataccg cgggcccggg
     2761 atccgatatc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     2821 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     2881 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     2941 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     3001 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggttggtatc
     3061 aaagatctgg atctataact tcgtatagca tacattatac gaagttatgg ttacaagaca
     3121 ggtttaagga gaccaataga aactgggcat gtggagacag agaagactct tgggtttctg
     3181 ataggcactg actctctctg cctattggtc tattttccca cccttaggtg agtaagggcg
     3241 aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
     3301 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     3361 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
     3421 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
     3481 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
     3541 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc
     3601 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     3661 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
     3721 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
     3781 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
     3841 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
     3901 tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca
     3961 acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga
     4021 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     4081 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     4141 cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca
     4201 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     4261 gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
     4321 agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
     4381 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
     4441 aggccctgaa gctgaaggac ggcggccgct acctggtgga gttcaagacc atctacatgg
     4501 ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct
     4561 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
     4621 tgttcctgta cggcatggac gagctgtaca agagatcctt agagcagaaa ctcattagcg
     4681 aggaggacct gaacagcgaa cagaaactca tttccgaaga ggatctcaac tccgagcaga
     4741 agctgatcag cgaggaggac ctgagatcct gagaattcta gataactgat cataatcagc
     4801 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4861 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4921 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4981 agttgtggtt tgtccaaact catcaatgta tcttatttcg ggccccccct cgaggtcgac
     5041 atgcccgccg tgaccgtcga gaacccgctg acgctgcccc gcgtatccgc acccgccgac
     5101 gccgtcgcac gtcccgtgct caccgtgacc accgcgccca gcggtttcga gggcgagggc
     5161 ttcccggtgc gccgcgcgtt cgccgggatc aactaccgcc acctcgaccc gttcatcatg
     5221 atggaccaga tgggtgaggt ggagtacgcg cccggggagc ccaagggcac gccctggcac
     5281 ccgcaccgcg gcttcgagac cgtgacctac atcgtcgacg gtatcgataa gcttgatatc
     5341 gaattcctgc agccgggctg caggaattct accgggtagg ggaggcgctt ttcccaaggc
     5401 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     5461 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     5521 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     5581 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     5641 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     5701 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     5761 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     5821 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     5881 tcccgccacc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     5941 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     6001 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     6061 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     6121 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     6181 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     6241 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     6301 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     6361 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     6421 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     6481 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     6541 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     6601 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     6661 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     6721 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     6781 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     6841 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     6901 gagggcaaag gaatagagta tgcagaaatt gatgatctat taaacaataa agatgtccac
     6961 taaaatggaa gtttttcctg tcatactttg ttaagaaggg tgagaacaga gtacctacat
     7021 tttgaatgga aggattggag ctacgggggt gggggtgggg tgggattaga taaatgcctg
     7081 ctctttactg aaggctcttt actattgctt tatgataatg tttcatagtt ggatatcata
     7141 atttaaacaa gcaaaaccaa attaagggcc agctcattcc tcccactcat gatctataga
     7201 tctatagatc tctcgtggga tcattgtttt tctcttgatt cccactttgt ggttctaagt
     7261 actgtggttt ccaaatgtgt cagtttcata gcctgaagaa cgagatcagc agcctctgtt
     7321 ccacatacac ttcattctca gtattgtttt gccaagttct aattccatca gaagctaaat
     7381 tgggcgcgcc caactagttc tagagcggcc gcaacttcgt cgacaaagga tccgtcggcg
     7441 accctacgcc cccaactgag agaactcaaa ggttacccca gttggggcac tactcccgaa
     7501 aacgtcggcg accctacgcc cccaactgag agaactcaaa ggttacccca gttggggcac
     7561 tactcccgaa aacgtcggcg accctacgcc cccaactgag agaactcaaa ggttacccca
     7621 gttggggcac tactcccgaa aacgatatcc ttacggaata ccactgaaat tgggcgcgga
     7681 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcctag ttctagagcg
     7741 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa tttcgagctt
     7801 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7861 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7921 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7981 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8641 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8881 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8941 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9001 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9061 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9121 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9181 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9241 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9301 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9361 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9421 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9481 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9541 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9601 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9661 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctc
//
