LOCUS       sequence-461934-        4517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0219h is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP15263 - pAAV-AiE0219h-minBG-iCre(R297T)-BGH...
FEATURES             Location/Qualifiers
     source          1..4517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      453..462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             465..485
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1551..1662
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1780..1920
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1780..1909
                     /label=AAV2 ITR
     rep_origin      1995..2450
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2082..2101)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2292..2313
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2467..2486)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2586..2608
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2646..2664)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2732..2836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2837..2905,2906..3697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3055..3074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3868..4456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4357..4376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttggtct tgatcaattg tcccagcaga gttggtgcat
      181 gggaaagcct ttcttggcct tatcagcagc tcagcctggg aggttggccc gggggctgac
      241 caagcacaaa atagaaaagc cactaataag atccagctgg aacaaatagc ctctatttat
      301 tttaggaaac taatgatatg tcatcatgac acatatctaa ccttagtatt tctgattgga
      361 caggccagtt agagctcggg ctgggcataa aagtcagggc agagccatct attgcttaca
      421 tttgcttctg ggatccagat ctttcgaagc tagccaccat ggtgcccaag aagaagagga
      481 aagtctccaa cctgctgact gtgcaccaaa acctgcctgc cctccctgtg gatgccacct
      541 ctgatgaagt caggaagaac ctgatggaca tgttcaggga caggcaggcc ttctctgaac
      601 acacctggaa gatgctcctg tctgtgtgca gatcctgggc tgcctggtgc aagctgaaca
      661 acaggaaatg gttccctgct gaacctgagg atgtgaggga ctacctcctg tacctgcaag
      721 ccagaggcct ggctgtgaag accatccaac agcacctggg ccagctcaac atgctgcaca
      781 ggagatctgg cctgcctcgc ccttctgact ccaatgctgt gtccctggtg atgaggagaa
      841 tcagaaagga gaatgtggat gctggggaga gagccaagca ggccctggcc tttgaacgca
      901 ctgactttga ccaagtcaga tccctgatgg agaactctga cagatgccag gacatcagga
      961 acctggcctt cctgggcatt gcctacaaca ccctgctgcg cattgccgaa attgccagaa
     1021 tcagagtgaa ggacatctcc cgcaccgatg gtgggagaat gctgatccac attggcagga
     1081 ccaagaccct ggtgtccaca gctggtgtgg agaaggccct gtccctgggg gttaccaagc
     1141 tggtggagag atggatctct gtgtctggtg tggctgatga ccccaacaac tacctgttct
     1201 gccgggtcag aaagaatggt gtggctgccc cttctgccac ctcccaactg tccacccggg
     1261 ccctggaagg gatctttgag gccacccacc gcctgatcta tggtgccaag gatgactctg
     1321 ggcagagata cctggcctgg tctggccact ctgccagagt gggtgctgcc accgacatgg
     1381 ccagggctgg tgtgtccatc cctgaaatca tgcaggctgg tggctggacc aatgtgaaca
     1441 ttgtgatgaa ctacatcaga aacctggact ctgagactgg ggccatggtg aggctgctcg
     1501 aagatgggga ctgaggcgcg ccgaattcaa gcttctcgag agatcttcga ctgtgccttc
     1561 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     1621 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     1681 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     1741 tagcaggcat gagatctcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     1801 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     1861 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     1921 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     1981 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     2041 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
     2101 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     2161 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     2221 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2281 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
     2341 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
     2401 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     2461 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     2521 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     2581 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     2641 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     2701 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     2761 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2821 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2881 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     2941 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3001 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3061 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3121 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3181 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3241 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3301 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3361 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3421 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3481 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3541 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3601 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3661 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     3721 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3781 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3841 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3901 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3961 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4021 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4081 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4141 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4201 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4261 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4321 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4381 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4441 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4501 gccttttgct cacatgt
//
