LOCUS       sequence-46195-e       11313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pMADM-alpha
FEATURES             Location/Qualifiers
     source          1..11313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(122..226)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(252..707)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(389..410)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     601..620
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     861..883
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     875..892
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     876..892
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     902..921
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        902..920
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        996..1299
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1301..1578
                     /label=chicken beta-actin promoter
     intron          1580..2597
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2521..2543
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2605..2624
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(2679..2712)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      2730..2739
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2781..2802)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(3027..3060)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3088..3192
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3194..3551
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3194..3385
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      3402..3537
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3586..4380
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3586..4380
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4612..4659
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(4845..4878)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4998..6422
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVL
                     KGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYER
                     SEGRHHLFLYGMDELYK"
     CDS             6432..6461
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6468..6497
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6504..6533
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(6549..6582)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      6604..6613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6630..6663)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6691..6795
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6797..7154
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6797..6988
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      7005..7140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7189..7983
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7189..7983
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    8215..8262
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(8448..8481)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8633..8652)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8980..9001
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(9071..9104)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    9226..9347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9263..9282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9317..9336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9374..9394)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9374..9392)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9413..9429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9413..9429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9426..9448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9437..9453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9461..9467,9468..9485,9486..9491))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9506..9527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9644..9661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9815..10403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9895..9914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10574..11313,1..52,53..121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11197..11216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
       61 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
      121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      241 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
      301 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
      361 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
      421 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
      481 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
      541 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
      601 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
      661 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
      721 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      781 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt ttcgattaag
      841 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
      901 gtaatacgac tcactatagg gcgaattggg gaatctagtt attaatagta atcaattacg
      961 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1021 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1081 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     1141 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     1201 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     1261 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     1321 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     1381 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg
     1441 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
     1501 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     1561 cgaagcgcgc ggcgggcggg gagtcgctgc gacgctgcct tcgccccgtg ccccgctccg
     1621 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     1681 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt
     1741 cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga
     1801 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc
     1861 tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc
     1921 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca
     1981 aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg
     2041 ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     2101 cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     2161 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
     2221 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     2281 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     2341 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     2401 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     2461 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     2521 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     2581 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     2641 gcaaagaatt gatttgatac cgcgggccca tcgaatttga agttcctata ctttctagag
     2701 aataggaact tccccgggat ccgatatccg ccaccatggt gagcaagggc gaggagctgt
     2761 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     2821 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     2881 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     2941 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     3001 tgcccgaagg ttggtatcaa agatctataa cttcgtatag catacattat acgaagttat
     3061 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3121 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3181 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     3241 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3301 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3361 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3421 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3481 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3541 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     3601 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3661 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3721 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     3781 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3841 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3901 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3961 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4021 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4081 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     4141 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4201 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4261 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4321 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4381 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     4441 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     4501 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     4561 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     4621 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     4681 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     4741 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4801 tcggggcggc aggccctgcc atagcctcag gttactcagg atctataact tcgtatagca
     4861 tacattatac gaagttatgg ttacaagaca ggtttaagga gaccaataga aactgggcat
     4921 gtggagacag agaagactct tgggtttctg ataggcactg actctctctg cctattggtc
     4981 tattttccca cccttaggtg agtaagggcg aggaggtcat caaagagttc atgcgcttca
     5041 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     5101 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     5161 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     5221 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     5281 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     5341 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     5401 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     5461 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     5521 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     5581 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     5641 agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg
     5701 gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc
     5761 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     5821 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     5881 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     5941 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     6001 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     6061 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     6121 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     6181 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccgct
     6241 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     6301 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     6361 agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca
     6421 agagatcctt agagcagaaa ctcattagcg aggaggacct gaacagcgaa cagaaactca
     6481 tttccgaaga ggatctcaac tccgagcaga agctgatcag cgaggaggac ctgagatcct
     6541 gagaattcga agttcctata ctttctagag aataggaact tcactagtgg atcccccggg
     6601 ttcgccacca tggttggtat caaagatcta taacttcgta tagcatacat tatacgaagt
     6661 tattcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6721 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6781 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     6841 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6901 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6961 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7021 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7081 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7141 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     7201 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     7261 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     7321 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     7381 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     7441 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     7501 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     7561 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     7621 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     7681 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     7741 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     7801 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     7861 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     7921 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     7981 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     8041 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     8101 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     8161 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     8221 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     8281 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     8341 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     8401 acgtcggggc ggcaggccct gccatagcct caggttactc aggatctata acttcgtata
     8461 gcatacatta tacgaagtta tggttacaag acaggtttaa ggagaccaat agaaactggg
     8521 catgtggaga cagagaagac tcttgggttt ctgataggca ctgactctct ctgcctattg
     8581 gtctattttc ccacccttag ggtacgtcca ggagcgcacc atcttcttca aggacgacgg
     8641 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     8701 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     8761 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggcgaa
     8821 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
     8881 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     8941 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     9001 gaccgccgcc gggatcactc tcggcatgga cgagctttac aaggatatcc ctgaattctg
     9061 atagaagctt gaagttccta tactttctag agaataggaa cttcgtcgac tagataactg
     9121 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     9181 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     9241 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9301 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttattt aaattgagct
     9361 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     9421 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9481 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9541 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9601 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10201 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    10261 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10321 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    10381 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    10441 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    10501 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    10561 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    10621 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10681 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10741 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10801 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10861 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10921 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10981 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    11041 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    11101 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    11161 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    11221 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    11281 cgctgttgag atccagttcg atgtaaccca ctc
//
