LOCUS       sequence-462032-        4902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2051m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP1343 - pAAV-AiE2051m-minBG-iCre
FEATURES             Location/Qualifiers
     source          1..4902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      424..433
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             436..456
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1505..1981
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1704..1723)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2021..2161
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2021..2150
                     /label=AAV2 ITR
     rep_origin      2236..2691
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2323..2342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2533..2554
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2708..2727)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2827..2849
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2887..2905)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2973..3077
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3078..3146,3147..3938)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3296..3315)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4109..4697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4598..4617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4759..4888
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 ccttttagaa acactccctt taccatcttc aaacatgttt ttagctgaga actctcttcc
       61 aacccctgtt tcttaactct gagtgccgta tcacttcata ttctgtgtca ggggaaaaga
      121 aatcagcacc tcccacaacg tcaactgtgt cctttagagt aagcattcca aagtctatat
      181 tctgagtcta atctctcttg gaaaggtcag ccttttcttc caaataccaa gtggacatct
      241 ttatgcacag gccttcaagg atattcaagt cagtgtgtcc tacggagaaa actcctttta
      301 aaacccgaat cctgtggttt ccccaagatg attattgggt ggtcactgca gccttaagga
      361 gctcgggctg ggcataaaag tcagggcaga gccatctatt gcttacattt gcttctggga
      421 tccgccacca tggtgcccaa gaagaagagg aaagtctcca acctgctgac tgtgcaccaa
      481 aacctgcctg ccctccctgt ggatgccacc tctgatgaag tcaggaagaa cctgatggac
      541 atgttcaggg acaggcaggc cttctctgaa cacacctgga agatgctcct gtctgtgtgc
      601 agatcctggg ctgcctggtg caagctgaac aacaggaaat ggttccctgc tgaacctgag
      661 gatgtgaggg actacctcct gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa
      721 cagcacctgg gccagctcaa catgctgcac aggagatctg gcctgcctcg cccttctgac
      781 tccaatgctg tgtccctggt gatgaggaga atcagaaagg agaatgtgga tgctggggag
      841 agagccaagc aggccctggc ctttgaacgc actgactttg accaagtcag atccctgatg
      901 gagaactctg acagatgcca ggacatcagg aacctggcct tcctgggcat tgcctacaac
      961 accctgctgc gcattgccga aattgccaga atcagagtga aggacatctc ccgcaccgat
     1021 ggtgggagaa tgctgatcca cattggcagg accaagaccc tggtgtccac agctggtgtg
     1081 gagaaggccc tgtccctggg ggttaccaag ctggtggaga gatggatctc tgtgtctggt
     1141 gtggctgatg accccaacaa ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc
     1201 ccttctgcca cctcccaact gtccacccgg gccctggaag ggatctttga ggccacccac
     1261 cgcctgatct atggtgccaa ggatgactct gggcagagat acctggcctg gtctggccac
     1321 tctgccagag tgggtgctgc cagggacatg gccagggctg gtgtgtccat ccctgaaatc
     1381 atgcaggctg gtggctggac caatgtgaac attgtgatga actacatcag aaacctggac
     1441 tctgagactg gggccatggt gaggctgctc gaggatgggg actaaggctg ctcgagagat
     1501 ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
     1561 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
     1621 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
     1681 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
     1741 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga
     1801 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
     1861 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
     1921 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
     1981 tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     2041 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     2101 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     2161 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     2221 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2281 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     2341 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2401 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     2461 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     2521 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     2581 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     2641 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     2701 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     2761 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     2821 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     2881 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     2941 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3001 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3061 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3121 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3181 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3241 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3301 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3361 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3421 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3481 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3541 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3601 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3661 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3721 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3781 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3841 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3901 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3961 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4021 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4081 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4141 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4201 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4261 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4321 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4381 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4441 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4501 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4561 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4621 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4681 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4741 ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc
     4801 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
     4861 gtggccaact ccatcactag gggttcctgc ggccgcacgc gt
//
