LOCUS       sequence-462037-        4901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2114m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle T, Dwivedi D, Way SW, Oster
            A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya X, 
            Roth JR, Allen S, Ayala A, Bakken TE, Barcelli T, Barta S, Bendrick 
            J, Bertagnolli D, Bowlus J, Boyer G, Brouner K, Casian B, Casper T, 
            Chakka AB, Chakrabarty R, Chance RK, Chavan S, Departee M, Donadio 
            N, Dotson N, Egdorf T, Gabitto M, Garcia J, Gary A, Gasperini M, 
            Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, Halterman C, 
            Helback O, Hockemeyer D, Huang C, Huff S, Hunker A, Johansen N, 
            Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, 
            Lee AY, Leibly M, Lenz GH, Liang E, Lusk N, Malone J, Mollenkopf T, 
            Morin E, Newman D, Ng L, Ngo K, Omstead V, Oyama A, Pham T, Pom CA, 
            Potekhina L, Ransford S, Rette D, Rimorin C, Rocha D, Ruiz A, 
            Sanchez REA, Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shulga L, 
            Sigler AR, Siverts LA, Somasundaram S, Stewart K, Szelenyi E, Tieu 
            M, Trader C, van Velthoven CTJ, Walker M, Weed N, Wirthlin M, Wood 
            T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch
            K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan 
            B, Yao S, Lein ES, Zeng H, Levi BP, Ngai J, Ting J, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types (preprint).
  JOURNAL   bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 
            10.1101/2024.06.10.597244.
  PUBMED    38915722
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 
            10.1101/2024.06.10.597244."
COMMENT     Sequence Label: AiP1911 - pAAV-AiE2114m-minBG-iCre-WPRE-BGHpA
FEATURES             Location/Qualifiers
     source          1..4901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             611..631
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1728..1748)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    1935..2046
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2164..2304
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2164..2293
                     /label=AAV2 ITR
     rep_origin      2379..2834
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2466..2485)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2676..2697
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2851..2870)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2970..2992
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3030..3048)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3116..3220
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3221..3289,3290..4081)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3439..3458)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4252..4840
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4741..4760
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttggca tttgacattt acctgaagtc tgtcttgtgc
      181 cgggtcctgt ccccaggttg ggtctaagtc tcacgctgga tctcagtgct cacagcttcc
      241 tccagaacat acctgctctg gcattatctt tcctgagcag atgctagaac ttggctcaga
      301 gtgatgaaga ggaagtgtgt cctctggcta aagtccaggg gttgcagagg ggagtgtgtg
      361 agatatttta atacatacct atcttgagaa ggtagcaggg gtgttaaagc atatgtaatc
      421 atccaggctt cttcatatct caagaaatct agcatagcac aaagatgggc tagatgtacc
      481 caaaggtgca tgcaaaacgt aagcagcttc ttgtttccta tttcactgag tttgagctcg
      541 ggctgggcat aaaagtcagg gcagagccat ctattgctta catttgcttc tgggatccgc
      601 caccatggtg cccaagaaga agaggaaagt ctccaacctg ctgactgtgc accaaaacct
      661 gcctgccctc cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt
      721 cagggacagg caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc
      781 ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt
      841 gagggactac ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca
      901 cctgggccag ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa
      961 tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc
     1021 caagcaggcc ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa
     1081 ctctgacaga tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct
     1141 gctgcgcatt gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg
     1201 gagaatgctg atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa
     1261 ggccctgtcc ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc
     1321 tgatgacccc aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc
     1381 tgccacctcc caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct
     1441 gatctatggt gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc
     1501 cagagtgggt gctgccaggg acatggccag ggctggtgtg tccatccctg aaatcatgca
     1561 ggctggtggc tggaccaatg tgaacattgt gatgaactac atcagaaacc tggactctga
     1621 gactggggcc atggtgaggc tgctcgagga tggggactaa gaattcgata tcataatcaa
     1681 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     1741 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     1801 ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc
     1861 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggct
     1921 cgagagatct tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     1981 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     2041 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2101 caagggggag gattgggaag acaatagcag gcatgagatc tcacgtgcgg accgagcggc
     2161 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     2221 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     2281 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     2341 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     2401 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     2461 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     2521 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     2581 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     2641 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2701 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     2761 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     2821 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2881 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     2941 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3001 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3061 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3121 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3181 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3241 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3301 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3361 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3421 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3481 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3541 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3601 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3661 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3721 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3781 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3841 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3901 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3961 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4021 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4081 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4141 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4201 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4261 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4321 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4381 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4441 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4501 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4561 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4621 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4681 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4741 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4801 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4861 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
