LOCUS       sequence-46207-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Burd CG, Emr SD
  TITLE     Phosphatidylinositol(3)-phosphate signaling mediated by specific 
            binding to RING FYVE domains.
  JOURNAL   Mol Cell. 1998 Jul;2(1):157-62.
  PUBMED    9702203
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "1998-07"; volume: "2(1)"; pages: "157-62"
COMMENT     Sequence Label: pRS424GFP-FYVE(EEA1)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             313..501
                     /codon_start=1
                     /product="FYVE domain of human early endosome antigen 1"
                     /label=FYVE(EEA1)
                     /note="binds PtdIns(3)P (Burd and Emr, 1998)"
                     /translation="WAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTP
                     SSKKPVRVCDACFNDLQG"
     primer_bind     complement(702..721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(703..721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(731..748)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(731..747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(740..762)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tccggaccca gatctcgagc tcaagcttcg aattcgtggc aatctagtca acggagagtt
       61 agtgagcttg agaaacaaac ggatgactta cggggtgaaa ttgcagtatt agaagcaacg
      121 gttcagaata atcaagatga aaggagagca ctactggaaa gatgtcttaa aggagaaggt
      181 gaaatagaaa agcttcaaac caaagtatta gaattgcaaa gaaagctgga taatacaact
      241 gcagcagtgc aggagctggg cagagaaaac caatcacttc agatcaaaca tacacaagcg
      301 ttgaatagaa agtgggccga agacaatgaa gtacaaaact gtatggcctg tgggaaaggc
      361 ttttcagtaa cagtgagacg gcatcactgc cgacagtgtg gaaatatctt ctgtgctgaa
      421 tgttcagcca aaaatgcctt aactccttcc tccaagaagc ctgttcgtgt ctgtgatgca
      481 tgtttcaatg acttgcaagg ataatgggtt atcacaactt cagagtaata ttacactaac
      541 attagatttt taataaatgt acttaataga ggtcttggac tactatttgg tttggacact
      601 ggttgcaata ctagaccaaa taggaattag tatattttgg aatgttatgg atgggggatc
      661 ctctagagtc gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt
      721 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      781 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      841 gcaccgatcg
//
