LOCUS       sequence-462094-        9177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression of opsin 'Chrimson', regulated by rab-3 
            promoter and unc-54 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-rab-3P::CHRIMSON_CE
FEATURES             Location/Qualifiers
     source          1..9177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6639..6661)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6761..6780
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6858..6876)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6944..7048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7049..7117,7118..7909)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7267..7286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8080..8668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8569..8588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8822..8839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8956..8977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8992..8997,8998..9015,9016..9022)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9030..9046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9035..9057
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9054..9070
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9054..9070
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctaacaact tggaaatgaa ataagcttgc atgcctgcag gcgagttttg actggctttc
       61 aaaaactgag tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag
      121 acatttttgc aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct
      181 ataatagttt ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt
      241 gaaagtatgc agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata
      301 taatctaggt taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc
      361 tttcaaatgg tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc
      421 ggatttattc caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt
      481 gaaaaaatga gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg
      541 agggttccgt gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag
      601 ccacactgtt ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag
      661 atgctttcat tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa
      721 ttatgcatga gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg
      781 tgtggtttaa tctgtgaaag ttttgaatct tctgtttgaa aaaaaaaaca tttttaattt
      841 gtatatacga gtgaattggt taggccctgg ttacgttttc cagcgaattc attcaaattt
      901 cttatttttt ctattttttc aattaaataa tttagttctc tatcacatgt gtatgatcaa
      961 aatttcattc ctaatcgtaa gcaaatttag gctcatccgt agtataattt tgttatgaag
     1021 ggcttcacag taaaagctca aactccaaca tttagcaact agcaaccaac aaaaaaccaa
     1081 agaaatgtca gttttgaaaa gtcgttattt ctttgtcttc tcacaattca cacatcatct
     1141 tctcgttttc ctccgttttt ttgtcgtgct tccttctttt ctttacacat ttttctaacc
     1201 tttttttaag tctatcctct cctaatcctt tgtactctct aatcagtttt gctggttgtg
     1261 ctgcacaatt tttatcagat ttatgcgcga aacaaaagcc tctcgcggag agctccgcca
     1321 aatccggtct tcttttttcc ttgcctatcg gatttcacaa acattcagtt tgtcacttga
     1381 cgatgtgttg gaggcattac tactaggaag tgttattcat attttttatt cgtttctttt
     1441 tggtgactaa acgaaatatt ccgaacgttt tcctgatttc aggcggcgga aacgaagaag
     1501 accacgaata atcaacaggc tgccattgca tccgccagaa gtcgagtgag ttctttgaaa
     1561 atctttatta gagttatttg tgacaatttg ccactttttt tttaatgttt aatgaatctg
     1621 taacgaaatt aataccaagg gtgtccaaca gaactatctg agcggttatt taatttttag
     1681 atagtatatt tcttccgatc gtaacataca tattttcaag catagtactg tgatttaagt
     1741 tacagttttc caatcagtct tgattttaat aaaaactttt caactgcaaa aatgcctctg
     1801 aagtattttt ctatattttt atagttgaat aattttttga ttggcacaaa gaccgccgag
     1861 atatgagccg ttaaagtaaa gctgcatgga ttcaatatgg tttgtatttg attggagtca
     1921 cttacatgga aatatttgta tttaaatctc atattttaaa acaagacaaa caccaaatta
     1981 aatttttatt aatctgaaca tttttcaatc gatttggtaa acaaattatt agatgtttcc
     2041 ctcaaaaatt gtaacaattc ctaaattgtg gcacttataa taacatcgtc ctgaattctc
     2101 aaaaaaaacc gaatgctaag agagatttcg tttaactggg acctgtaact gaattttcaa
     2161 atattggcag tttttgataa actgggaggg aaatcaaaaa ccaaaactac agtacccctc
     2221 gtgttataat aaattgtcat tcactccttt tttgttccca acatttgctc ccatctcctg
     2281 acgagaaaga acaattttat gagcacggga acccgtgggc gtccatccgt tcctcctgtg
     2341 caatttcagc ccccttcctc tacattctct gtgttcccgt ttttatcgtt ttatgggtat
     2401 cgggaacgga cagatcgctt ccatttgctt ctattccgtc ctcacaagac gtcccactaa
     2461 ctgattttct tctgatttct tttcactgaa aatgggaaag aaagaaaaag acgatgaatc
     2521 gacgacttct tctcaggaga aggtgaagaa gaaggataag gagaaagttc atctaaggct
     2581 gaaagataaa gtaaaaaaac ttagaaaatc taagtcgatt gcagtatctt ctccagctga
     2641 ttatatgact tgtcagtgtg aaccatgcaa aaaagtagct ctcgaggtaa taatttttaa
     2701 actcaatacg tagttaagtt tgaactcctt ttaatttact tatcatctaa tttaccgtca
     2761 caatagtact tccattagca attattttaa aattacatgc atagcttcaa tgttcgtttt
     2821 tttgcgttct catatcgtat gttgtctgcc tctcgaatac tgactgtccg actagaatgt
     2881 cccggtagga ttactgtacg gtcactggga ataaactgtt tctgaaatca atctgaaatt
     2941 ccgtagatct tcttggttca atttcttatt cctcaatctt tggacaacgt caccttcaga
     3001 gtcgtcgttg agtatcccat cattaactag tcaatctttc tctctttttc atttccccca
     3061 acttttctgc atattttttg acgacgacga cctcgacggc aacgagaata catttatcta
     3121 ttttttagta tagcatatat acattttcta cttttctcta acccatttca gacgtataga
     3181 ttttggtttt cgaatgcaac tttgagattt ttcaaagatc ttcagatggg agcagtggac
     3241 tgtctatttg atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc
     3301 ctacataaat atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt
     3361 tgcgatatta ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat
     3421 taagttggct tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt
     3481 taaaacttta ttattgacta tttatatata ttatatatta tatattttca tggtttaatc
     3541 aaaatctatt aaattatttc taagatctct aaatatcgaa ttctctaaaa agcttcatca
     3601 acaaaagcat ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa
     3661 atctagaaaa aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa
     3721 gcgcctacac gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc
     3781 atacactcct cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc
     3841 ggttgtgatg tatgcatcgg gcgtttctat ccctctccgt gagcaacgag ctagtcaacc
     3901 catcgatcca tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca
     3961 ctgagctcca agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg
     4021 tcttcttctt ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt
     4081 cctccagaga aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat
     4141 gataaaaatt tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt
     4201 gggaaattaa aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca
     4261 gacatttctt atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa
     4321 gatatctcgc tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat
     4381 ctaaatgctc ttttaaaata aatctacagt agccctattt tcagggatcc ccgggattgg
     4441 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     4501 gacccttgga gggtaccgag ctcagaaaaa atggccgaac tcatctcatc ggctacacga
     4561 tcacttttcg ctgccggagg aatcaatcca tggccaaatc cataccacca cgaggatatg
     4621 ggatgcggag gaatgacacc aactggagag tgcttctcaa cagagtggtg gtgcgatcca
     4681 tcatacggac tttcagatgc cggatacgga tactgcttcg ttgaagctac tggaggatac
     4741 ctcgttgtgg gagtcgaaaa gaaacaagcc tggctccatt cacgaggaac accaggtaag
     4801 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcaggaga aaagatcgga
     4861 gcccaagtgt gccaatggat cgctttctca attgctatcg ccctcctcac attctacgga
     4921 ttctcagctt ggaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     4981 tttcaggcta catgcggatg ggaagaggtt tacgtttgct gcgttgaggt cctcttcgtg
     5041 acactcgaga tcttcaaaga gttctcgtcg ccagctacag tgtacctctc aactggaaat
     5101 cacgcttact gcctccgata cttcgaatgg cttctctcgt gcccagtgat cctcatcaag
     5161 ctctcaaatc tctcgggact caagaacgat tactcgaagc gaacaatggg actcatcgtg
     5221 tcgtgcgtgg gaatgattgt gttcggaatg gctgctggac tcgctacaga ttggcttaaa
     5281 tggctcctct acatcgtgtc atgcatctac ggaggatata tgtacttcca agccgccaag
     5341 tgctacgttg aggctaatca ctcagttcca aaggtaagtt taaacagttc ggtactaact
     5401 aaccatacat atttaaattt tcagggacac tgccgaatgg ttgtgaagct tatggcttac
     5461 gcctacttcg cctcatgggg atcataccca attctctggg ctgttggacc agaaggactc
     5521 ctcaaacttt cgccatacgc caactcgatc ggacactcga tctgcgatat tatcgccaaa
     5581 gaattctgga cattcctcgc ccatcacctc cgaatcaaga ttcacgagca catcctcatc
     5641 cacggagata tccgaaagac aacaaagatg gaaatcggag gagaagaggt cgaggtcgaa
     5701 gaattcgtgg aagaggaaga tgaggataca gtctaagcgc cggtcgctac cattaccaac
     5761 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     5821 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     5881 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     5941 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     6001 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     6061 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     6121 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     6181 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     6241 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     6301 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     6361 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     6421 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     6481 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     6541 tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg
     6601 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6661 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6721 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     6781 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     6841 atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6901 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6961 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7021 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7081 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7141 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7201 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7261 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7321 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7381 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7441 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7501 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7561 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7621 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7681 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7741 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7801 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7861 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7921 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7981 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8041 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8101 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8161 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8221 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8281 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8341 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8401 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8461 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8521 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8581 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8641 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8701 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8761 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8821 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8881 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     8941 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     9001 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9061 cagctatgac catgattacg ccaagctgta agtttaaaca tgatcttact aactaactat
     9121 tctcatttaa attttcagag cttaaaaatg gctgaaatca ctcacaacga tggatac
//
