LOCUS       sequence-462108-        7445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of 'GUR-3' on activation via 'QF+hGR' chimeric protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-5xQUAS::Delta-pes-10P::AI::gur-3G
FEATURES             Location/Qualifiers
     source          1..7445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4907..4929)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5029..5048
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5126..5144)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5212..5316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5317..5385,5386..6177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5535..5554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6348..6936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6837..6856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7090..7107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7224..7245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7260..7265,7266..7283,7284..7290)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7298..7314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7303..7325
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7322..7338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7322..7338
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctaacaact tggaaatgaa ataagcttgc atgcctgcag gggtaatcgc ttatcctcgg
       61 ataaacaatt atcctcacgg gtaatcgctt atccgctcgg gtaatcgctt atcctcgggt
      121 aatcgcttat ccttaagctt gatatccctg caggatcgat tttttgcaaa ttacgagcgt
      181 tgtagggggc ggagcgatag gtcctatagg ttttggtata tcatcattca ttcattcatt
      241 ggtacattca tttacccacc ttcctctttc tgagcttctc tggagttctg tgcttccttt
      301 ttcccttatc tttatactgt aatttttaac tttcagggat ccccgggatt ggccaaagga
      361 cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg
      421 gagggtaccg agctcagaaa aaatgacgat aacagcgtcg aatacgttgg agttcaaatg
      481 gacgtcacca aggagctcga gatcgtcatt cagtaagttc actggaatga acctgtgcag
      541 ccttttattt ttcaacattg tttataggaa caacgacaga tgcggaacag aaaatatcaa
      601 ttgacatgag taatacttac tgtgatcagg taaatgatac agtgatggtt cgactctaaa
      661 aattactgag atttcgaata actaaaaaca gttttttgga atgattaaat aagtaaaaat
      721 tctaaatgta tcaacaatac gtgttgtttt gaataattga acataaaaga aaataagagg
      781 agacaaagtg tgttaagttg tgaaattttg actgaagcga cacattttca tcaaagggta
      841 ataatatgaa aaccataata gccctacatt tttgtgggtg caggtatatt tgagactgca
      901 cgcacggtgt gcctagattt atttttagtt tcaaatattt ttcatatttc cgaacgaata
      961 gtggaagcag caaaatgtat ttgtagcatc aagtatttct agccattttg caatttttat
     1021 attgttcagt tctaatgaag attgcagaaa aaaacggtaa caaacgctta ttcaagaaca
     1081 aattgtatat gttcaggttt tggggcctct gtactcctat atgatggtgt tgggacttaa
     1141 tcacactcac agtagcgctc ggaatacaat gtttaagtgg ccattgacaa tctacaatta
     1201 tctcactcta gccatattgg taagctacat tatgtcctga tcttcgatta aacaagattg
     1261 tactttctct gcgtgtgtgt aatacgagat gtttataaaa gtttagccca gcaagtggaa
     1321 cattttcaaa tttttccaga cagcggccac aattcgacgc atttcacaaa tcaaacaaaa
     1381 atcggcaaca aatgaggaaa aagatgctgc ttttcatgtg ctaaacccga cgtttgtgct
     1441 cacgttgtgt catgctttgc taatgttcag gttgtgtttt gcgcagcgtt aagttggtta
     1501 ttaaactcaa acatgaacaa acaaatataa cgatacatga aaatcgcaca aagaatacat
     1561 ggtttttgat tcgcgggcat aagattgtct agtttgctca aaagtatttg tatttcggga
     1621 gataattata tttaaaaaat tttgaaatgt tatggaagtg gtcgtttgat atcaagatcg
     1681 aacaattttt taaagatatg tgtttcctat tagtatggtg tacccataag aattgcaatc
     1741 tctaaacaat atttttaatg atatgaacat ttaatgatat tcttcattca tttttgagga
     1801 ttccttacta agtcacaaaa aaatcgtcga attaggtatt ttctagatgc caattcgagg
     1861 tttcaaaaag tgtcacacac aagttgaata taccttaata aaaacaagtt acttccaatt
     1921 tggaacaaac catcaaaact tagtattgta gtctcttaca gtctaatagt attccacgaa
     1981 aacatttccg ggaattcgta tggcacgcaa tactattaga aggtaaatca attttcataa
     2041 attataaacc aaaaaaaaat attgatggaa aattattgtt tgaacgtttc tgtagtctat
     2101 tttattaatt tattgtttta tctcgtatat gacttgttta attgacttgc attactagac
     2161 agtctcaatt tcctaaaaac gaactagttt ctaatttttt gaataagact ttagtttcat
     2221 ggaaattaag gtttattgga aaccaatcac aaagctaaca tcacaaattt attttaaatg
     2281 atatgcgctt tgtgttattc tcatatttaa atttcagtgg tcttgctgcc ggattccttt
     2341 tgctaaagtt gcaaaagcaa agagagaaaa tgtaccacgt gagtgaaatt agacacggtt
     2401 gaggtagatc acaagaccaa cagggcttga cttacaggtg ttagatcaag ggttgggtag
     2461 gaatagaaat gaagaacatg acagtcatca tttcaagctg aataagttat ttatatcaat
     2521 cagtttttcg tttgcagctg cattatcctt tgtacaaatt ggtaacaaaa aagtatgact
     2581 aattcgaaac cattcgaaaa atttccagct acaaaaatga gatatcttga tctccctgac
     2641 actcctgatc tgattaacag aaaaatctac tttgtgattc ttgaaggata cgtggtacgg
     2701 ttgatattct ttatgcttgc caaaaatttt caaaattaag attttcatcg cttccagttg
     2761 catttcatta gtcgcaatat tattttttca actatgtaga atactccaat tttcgatagg
     2821 acaactaata gaagaaatgg tgcctaaaga aaaagaagaa tgcccactac cagaacaatc
     2881 tttacaacaa attcacgatg ttcaaattca ttaccaagaa atttccaatg ctaagctgta
     2941 tatagaacag aatttctcat tttcactgtt ttatacctac ggctgttgta ttccgctgac
     3001 ctgtctgttg ggctacattg cttttcgaaa tggaattcag gtagtgaaaa tgagcagcaa
     3061 taccattgtt tcatatcatc atttaggctg atatggcgga aactttttct gtggcaatat
     3121 ggctcactaa tactatgtta gcattgatgc ttttctcgat tccagctttt atgattgctg
     3181 aagaggttag tacttataag tatgtatatg tttgtttaaa tacatagata tagttagagc
     3241 agaactgaga aaattgagtt agtaaagttt ttgtatttct tcaacttttg atgttagctg
     3301 aacaaaaatt aaaaaacaac ataaattaaa tataatgcaa gcatatttgt gaacaagaaa
     3361 tgtgtacgtg gataaagtcg cggtggaatt atcatttaac cgtgattgca tgcatataaa
     3421 tcccacaata cgtaaactca atttatttat gacagtttca caattacgat cagaaattta
     3481 aaataaatag gttttcaaac actattcttc agcgacaata tttctgctaa ttgtcgctga
     3541 tattttatgt tcgcaaacgc agtaattcaa aattgatatc caaacattaa gttgtacaaa
     3601 taaaaattaa taattacagg gcgacaaact tctaaccgct tctttcaaaa tgtatcacga
     3661 aactctatgt gaggaacgtg atcttctagt tttggtgtgt tcgttatctc aaaaaaaaat
     3721 cgatcaattc tactttcagt ctcaaatgag ttttctttcg tttcaaatgc atgctacaaa
     3781 gcttactctc accgccggga atttcttcat gatgaatcgc aaaattatga tcagtgtacg
     3841 tttaaaggcg tgcagctggt ttttagttga tatttaaaat atttcagttg ttctctgcaa
     3901 ttttcacata ttttcttatt ttggtacagt ttgatgctga aaaggagcga gccggagaat
     3961 gcaataatca atctcgtgtg ctcattgtcc aaccacctgt gtaagcgccg gtcgctacca
     4021 ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac
     4081 tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg
     4141 acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc
     4201 ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta
     4261 agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag
     4321 tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa
     4381 tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga
     4441 aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt
     4501 atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt
     4561 ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt
     4621 gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa
     4681 aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc
     4741 tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt
     4801 gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct actagtcggc
     4861 cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4921 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     4981 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     5041 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     5101 aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata ggttaatgtc
     5161 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5221 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5281 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5341 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5401 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5461 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5521 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5581 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5641 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5701 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5761 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5821 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5881 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5941 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6001 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6061 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6121 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6181 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6241 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6301 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6361 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6421 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6481 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6541 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6601 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6661 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6721 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6781 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6841 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6901 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6961 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7201 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7261 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7321 acaggaaaca gctatgacca tgattacgcc aagctgtaag tttaaacatg atcttactaa
     7381 ctaactattc tcatttaaat tttcagagct taaaaatggc tgaaatcact cacaacgatg
     7441 gatac
//
