LOCUS       sequence-462127-       11193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression of chimeric protein 'QF+hGR', regulated by 
            rab-3 promoter and unc-54 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 11193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-rab-3P::AI::QF+hGR
FEATURES             Location/Qualifiers
     source          1..11193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8655..8677)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8777..8796
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8874..8892)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8960..9064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9065..9133,9134..9925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9283..9302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10096..10684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10585..10604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10838..10855
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10972..10993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11008..11013,11014..11031,11032..11038)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11046..11062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11051..11073
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11070..11086
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11070..11086
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctaacaact tggaaatgaa ataagcttgc atgcctgcag gcgagttttg actggctttc
       61 aaaaactgag tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag
      121 acatttttgc aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct
      181 ataatagttt ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt
      241 gaaagtatgc agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata
      301 taatctaggt taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc
      361 tttcaaatgg tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc
      421 ggatttattc caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt
      481 gaaaaaatga gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg
      541 agggttccgt gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag
      601 ccacactgtt ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag
      661 atgctttcat tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa
      721 ttatgcatga gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg
      781 tgtggtttaa tctgtgaaag ttttgaatct tctgtttgaa aaaaaaaaca tttttaattt
      841 gtatatacga gtgaattggt taggccctgg ttacgttttc cagcgaattc attcaaattt
      901 cttatttttt ctattttttc aattaaataa tttagttctc tatcacatgt gtatgatcaa
      961 aatttcattc ctaatcgtaa gcaaatttag gctcatccgt agtataattt tgttatgaag
     1021 ggcttcacag taaaagctca aactccaaca tttagcaact agcaaccaac aaaaaaccaa
     1081 agaaatgtca gttttgaaaa gtcgttattt ctttgtcttc tcacaattca cacatcatct
     1141 tctcgttttc ctccgttttt ttgtcgtgct tccttctttt ctttacacat ttttctaacc
     1201 tttttttaag tctatcctct cctaatcctt tgtactctct aatcagtttt gctggttgtg
     1261 ctgcacaatt tttatcagat ttatgcgcga aacaaaagcc tctcgcggag agctccgcca
     1321 aatccggtct tcttttttcc ttgcctatcg gatttcacaa acattcagtt tgtcacttga
     1381 cgatgtgttg gaggcattac tactaggaag tgttattcat attttttatt cgtttctttt
     1441 tggtgactaa acgaaatatt ccgaacgttt tcctgatttc aggcggcgga aacgaagaag
     1501 accacgaata atcaacaggc tgccattgca tccgccagaa gtcgagtgag ttctttgaaa
     1561 atctttatta gagttatttg tgacaatttg ccactttttt tttaatgttt aatgaatctg
     1621 taacgaaatt aataccaagg gtgtccaaca gaactatctg agcggttatt taatttttag
     1681 atagtatatt tcttccgatc gtaacataca tattttcaag catagtactg tgatttaagt
     1741 tacagttttc caatcagtct tgattttaat aaaaactttt caactgcaaa aatgcctctg
     1801 aagtattttt ctatattttt atagttgaat aattttttga ttggcacaaa gaccgccgag
     1861 atatgagccg ttaaagtaaa gctgcatgga ttcaatatgg tttgtatttg attggagtca
     1921 cttacatgga aatatttgta tttaaatctc atattttaaa acaagacaaa caccaaatta
     1981 aatttttatt aatctgaaca tttttcaatc gatttggtaa acaaattatt agatgtttcc
     2041 ctcaaaaatt gtaacaattc ctaaattgtg gcacttataa taacatcgtc ctgaattctc
     2101 aaaaaaaacc gaatgctaag agagatttcg tttaactggg acctgtaact gaattttcaa
     2161 atattggcag tttttgataa actgggaggg aaatcaaaaa ccaaaactac agtacccctc
     2221 gtgttataat aaattgtcat tcactccttt tttgttccca acatttgctc ccatctcctg
     2281 acgagaaaga acaattttat gagcacggga acccgtgggc gtccatccgt tcctcctgtg
     2341 caatttcagc ccccttcctc tacattctct gtgttcccgt ttttatcgtt ttatgggtat
     2401 cgggaacgga cagatcgctt ccatttgctt ctattccgtc ctcacaagac gtcccactaa
     2461 ctgattttct tctgatttct tttcactgaa aatgggaaag aaagaaaaag acgatgaatc
     2521 gacgacttct tctcaggaga aggtgaagaa gaaggataag gagaaagttc atctaaggct
     2581 gaaagataaa gtaaaaaaac ttagaaaatc taagtcgatt gcagtatctt ctccagctga
     2641 ttatatgact tgtcagtgtg aaccatgcaa aaaagtagct ctcgaggtaa taatttttaa
     2701 actcaatacg tagttaagtt tgaactcctt ttaatttact tatcatctaa tttaccgtca
     2761 caatagtact tccattagca attattttaa aattacatgc atagcttcaa tgttcgtttt
     2821 tttgcgttct catatcgtat gttgtctgcc tctcgaatac tgactgtccg actagaatgt
     2881 cccggtagga ttactgtacg gtcactggga ataaactgtt tctgaaatca atctgaaatt
     2941 ccgtagatct tcttggttca atttcttatt cctcaatctt tggacaacgt caccttcaga
     3001 gtcgtcgttg agtatcccat cattaactag tcaatctttc tctctttttc atttccccca
     3061 acttttctgc atattttttg acgacgacga cctcgacggc aacgagaata catttatcta
     3121 ttttttagta tagcatatat acattttcta cttttctcta acccatttca gacgtataga
     3181 ttttggtttt cgaatgcaac tttgagattt ttcaaagatc ttcagatggg agcagtggac
     3241 tgtctatttg atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc
     3301 ctacataaat atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt
     3361 tgcgatatta ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat
     3421 taagttggct tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt
     3481 taaaacttta ttattgacta tttatatata ttatatatta tatattttca tggtttaatc
     3541 aaaatctatt aaattatttc taagatctct aaatatcgaa ttctctaaaa agcttcatca
     3601 acaaaagcat ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa
     3661 atctagaaaa aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa
     3721 gcgcctacac gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc
     3781 atacactcct cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc
     3841 ggttgtgatg tatgcatcgg gcgtttctat ccctctccgt gagcaacgag ctagtcaacc
     3901 catcgatcca tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca
     3961 ctgagctcca agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg
     4021 tcttcttctt ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt
     4081 cctccagaga aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat
     4141 gataaaaatt tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt
     4201 gggaaattaa aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca
     4261 gacatttctt atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa
     4321 gatatctcgc tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat
     4381 ctaaatgctc ttttaaaata aatctacagt agccctattt tcagggatcc ccgggattgg
     4441 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     4501 gacccttgga gggtaccgag ctcagaaaaa atgccaccaa aacgcaagac actcaatgcc
     4561 gctgccgaag ccaatgccca cgctgatggc catgctgatg gcaatgctga tggtcatgtc
     4621 gctaacactg cagcaagcag caacaacgcc cgttttgcgg acttgaccaa cattgacaca
     4681 cccggcctcg gccctaccac gacgacgtta cttgtcgagc ccgctcgttc gaaacgccag
     4741 agagtctcga gggcctgtga tcagtgtcga gctgcacgtg aaaagtgtga tggaatccag
     4801 ccggcttgct tcccctgtgt gtcgcagggc cggtcgtgta cctaccaggc cagtcccaag
     4861 aagcgaggag tccagacggg ctacatccgc actctcgaac tggctctggc ttggatgttc
     4921 gagaacgttg cccgcagcga ggacgccctc cacaatcttt tggtccgtga tgctggccag
     4981 ggcagcgctc tcctggtcgg caaagactcg cctgctgcag aacgcctcca tgcaagatgg
     5041 gcgacgagtc gagtcaacaa aagcatcacc cgtcttctct caggtcaggc cgcacaagat
     5101 ccatctgaag acggccaatc cccgtccgaa gacataaatg tccaagatgc cggggcaaag
     5161 acatccgact tccctcatgc gcctcacttg actttctcgg cgcctaagtc tagtacagct
     5221 gagacacgca ctctaccagg cccggtccga ccacctattt cggcaaacac cctggaaaac
     5281 aaccttcagc cagatggaac cgggataggg aagctaccac ccaatcattg gcgcctgctg
     5341 gatatctact tttcctacac gcattcttgg ctccctatcc tcgagaagaa agacatgtac
     5401 caagcattgt accagtactc tgaacaaggc tcgttgcttc cctctgcgaa tgtcgagtct
     5461 ggcgttcatg ccgagctttg gagcgcgctc gccctggcgt ccttccaggc tgctgctact
     5521 gctgcatcga gtgctacggg tccagcttca gctgctcatg gccatgacaa tgccatcaat
     5581 ccttcacctg cagacatatc tgacacagcc cgaaagctca tacctttgga aagcgggccg
     5641 ttccaggttc agcactgcag agcgttgctg cttctttgtc tcgtaagcct tgggcgggat
     5701 gattgggagt ctgcttggtt gctggttggc tttgcggtcc gcgtcctact tgttgttcgc
     5761 acccagttgc ctcctgatga tgaccggcca cgaccaagaa tgcgtgcgct gctcgtcgcg
     5821 tgcttcatcg tggataccat tgtgtctatg agacacaacg tgccggccca tctcaagcca
     5881 gacgacattg cggatctgcc gttacctgaa gacggtcaag atcaatggga gccgtggaca
     5941 ccatgtgagg gcttaggcgg tgaacacacc atgctgcaaa tgttgaggaa cccggcatac
     6001 cctttaagca cattcaacca cctatatggc gtgaccaagc tggttgcttt ggagcttctg
     6061 ccaagaatac gaacatcttc acagaacgct cccttggagt tcaggtcgcg gttgcagcag
     6121 gtaatcggcc acaattctcc cttcagcgtc tttgtccttt cccaggatac agcatcggct
     6181 tttgtgccta ctgcatacct tacccgtacc gtttatttat gggcagctgc cttttctgag
     6241 cctctcaacg aacactactc gcatcttctg atcgaaactc ttgatcagta tcagaagcgg
     6301 tttggtacat atgcaatccc acctctgatc ccttctcttc tagactccct tcttgcttta
     6361 aagaaacaat cacattcttc agagcggcat cgaaggcact tggaagagct tttccccgcc
     6421 tactcctcca tttggcctcg gggaggccga cacagcaata ctggcctcca acccatacga
     6481 caacttgagc ttccgccgac tgcgactgcc actgcaagta tcatgcccca tgtcatggaa
     6541 cagcccctgt caacgtcaat aaatccggtc aatgatcggt ttaatggaat accgaatcct
     6601 accccctaca atagcgatgc ggccctagac gcaattactc aaaccaatga ctacggatca
     6661 gtcaataccc atggcattct cagtacatat ccaccacctg ccacccactt gaacgaggcc
     6721 tcggtggccc ttgcgcctgg aggtgctcct cccagaccac ccccgccata cgttgatagt
     6781 acaacgaacc atcctcctta ccatagtaac ctcgtcccca tggccaattt cggatattcg
     6841 actgtagact atgatgcgat ggtggatgat ctggcatcga tcgagtacac ggatgcggtt
     6901 gacgttgatc cgcagttcat gacgaacctc gggtttgtcc cagggtgtaa ttttagcgat
     6961 atcaacacgt atgagcaagt tcctgcaacg ttaccacaac tcacccctac cctggtgtca
     7021 ctgttggagg ttattgaacc tgaagtgtta tatgcaggat atgatagctc tgttccagac
     7081 tcaacttgga ggatcatgac tacgctcaac atgttaggag ggcggcaagt gattgcagca
     7141 gtgaaatggg caaaggcaat accaggtttc aggaacttac acctggatga ccaaatgacc
     7201 ctactgcagt actcctggat gtttcttatg gcatttgctc tggggtggag atcatataga
     7261 caatcaagtg caaacctgct gtgttttgct cctgatctga ttattaatga gcagagaatg
     7321 actctaccct gcatgtacga ccaatgtaaa cacatgctgt atgtttcctc tgagttacac
     7381 aggcttcagg tatcttatga agagtatctc tgtatgaaaa ccttactgct tctctcttca
     7441 gttcctaagg acggtctgaa gagccaagag ctatttgatg aaattagaat gacctacatc
     7501 aaagagctag gaaaagccat tgtcaagagg gaaggaaact ccagccagaa ctggcagcgg
     7561 ttttatcaac tgacaaaact cttggattct atgcatgaag tggttgaaaa tctccttaac
     7621 tattgcttcc aaacattttt ggataagacc atgagtattg aattccccga gatgttagct
     7681 gaaatcatca ccaatcagat accaaaatat tcaaatggaa atatcaaaaa acttctgttt
     7741 catcaaaagt aggcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg
     7801 ggccgctgtc atcagagtaa gtttaaactg agttctacta actaacgagt aatatttaaa
     7861 ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct
     7921 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct
     7981 taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc
     8041 aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa
     8101 taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt
     8161 ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     8221 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     8281 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     8341 agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag
     8401 atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa
     8461 agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg
     8521 aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac
     8581 caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt
     8641 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     8701 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     8761 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     8821 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt
     8881 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     8941 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9001 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9061 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     9121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9181 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9241 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9301 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9361 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9421 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9481 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9541 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9601 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9661 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9721 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9781 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9841 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9901 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9961 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    10021 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10201 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10441 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10501 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10561 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10621 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10681 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10741 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    10801 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    10861 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    10921 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    10981 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    11041 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    11101 gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt tcagagctta
    11161 aaaatggctg aaatcactca caacgatgga tac
//
