LOCUS       sequence-462134-        9231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for generating stable lines allowing expression of 
            ZNF207-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Behera AK, Kim JJ, Kordale S, Damodaran AP, Kumari B, Vidak S, 
            Dickson E, Xiao MS, Duncan G, Andresson T, Misteli T, 
            Gonatopoulos-Pournatzis T
  TITLE     RNA-coupled CRISPR Screens Reveal ZNF207 as a Regulator of LMNA 
            Aberrant Splicing in Progeria.
  JOURNAL   bioRxiv [Preprint]. 2025 Apr 26:2025.04.25.648738. doi: 
            10.1101/2025.04.25.648738.
  PUBMED    40568141
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Apr 26:2025.04.25.648738. doi: 
            10.1101/2025.04.25.648738."
COMMENT     Sequence Label: pLenti-ZNF207-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2106..2130)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2132..2197
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2239..2827
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2292..2312)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2710..2721)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2899..3132
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3210..3331
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3247..3266)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3301..3320
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3371..3506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3526..3545)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3527..3545)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3555..3572)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3555..3571)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3564..3586)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3713..4168
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3800..3819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4010..4031
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4194..4298
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4299..4367,4368..5159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4517..4536)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5330..5918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5819..5838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6072..6089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6206..6227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6242..6247,6248..6265,6266..6272)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6280..6296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6285..6307
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6304..6320
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6304..6320
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6339..6359
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6341..6359
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6387..6613
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6614..6794
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6841..6966
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7459..7692
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7877..7921
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8096..8596
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8515..8533
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8608..9003
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9063..9180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagcaggct tcatgggtcg caagaagaag aagcagctga agccgtggtg
      661 ctggtattgt aatagagatt ttgatgatga gaagatcctt attcagcacc aaaaagcaaa
      721 gcattttaaa tgccatatat gtcacaagaa attgtataca ggacctggct tagctattca
      781 ttgcatgcag gtacataaag aaacaataga tgccgtacca aatgcaatac ctggaagaac
      841 agacatagag ttggaaatat atggtatgga aggtattcca gaaaaagaca tggatgaaag
      901 acgacgactt cttgaacaga aaacacaaga aagtcaaaaa aagaagcaac aagatgattc
      961 tgatgaatat gatgatgacg actctgcagc ctcaacttca tttcagccac agcctgttca
     1021 acctcagcaa ggttatattc ctccaatggc acagccagga ctgccaccag taccaggagc
     1081 accaggaatg cctccaggca tacctccatt aatgccaggt gttcctcctc tgatgccagg
     1141 aatgccacca gttatgccag gcatgccacc tggattgcat catcagagaa aatacaccca
     1201 gtcattttgc ggtgaaaaca taatgatgcc aatgggtgga atgatgccac ctggaccagg
     1261 aataccacct ctgatgcctg gaatgccacc aggtatgccc ccacctgttc cacgtcctgg
     1321 aattcctcca atgactcaag cacaggctgt ttcagcgcca ggtattctta atagaccacc
     1381 tgcaccaaca gcaactgtac ctgccccaca gcctccagtt actaagcctc ttttccccag
     1441 tgctggacag atggggacac ctgtcacaag ctcaagtaca gcttcatcca attcagaaag
     1501 tctgtctgca tcttctaaag ctctgtttcc tagcacagca caagctcagg cagctgtcca
     1561 aggacctgtt ggtacagatt tcaaaccctt aaatagtacc cctgcaacaa ctacagaacc
     1621 cccaaagcct acattccctg cttatacaca gtctacagct tcaacaacta gtacaacaaa
     1681 tagtactgca gctaaaccag cggcttcaat aacaagtaag cctgctacac ttacaactac
     1741 atccgccaca tcaaagttga tccatccaga tgaggatata tccctggaag agagaagggc
     1801 acagttacct aagtatcaac gtaatcttcc tcggccagga caggccccca tcggtaatcc
     1861 accagttgga ccaattggag gtatgatgcc accacagcca ggcatcccac agcaacaagg
     1921 aatgagaccc ccaatgccac ctcatggtca gtatggtggt catcatcaag gcatgccagg
     1981 ataccttcct ggtgctatgc ccccgtatgg gcagggaccg ccaatggtgc ccccttacca
     2041 gggtgggcct cctcgacctc cgatgggaat gagacctcct gtaatgtcgc aaggtggccg
     2101 ttacaaccca gctttcttgt acaaagtggt tgactacaag gaccacgacg gtgactacaa
     2161 ggaccacgac atcgactaca aggacgacga cgacaagtag taatgagcta gcgctaaccg
     2221 gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2281 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2341 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2401 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2461 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2521 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2581 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2641 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2701 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2761 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2821 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     2881 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     2941 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3001 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3061 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3121 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     3181 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3241 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3301 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     3361 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3421 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3481 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     3541 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3601 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3661 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3721 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3781 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3841 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3901 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3961 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4021 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4081 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4141 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4201 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4261 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4321 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4381 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4441 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4501 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4561 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4621 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4681 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4741 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4801 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4861 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4921 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4981 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5041 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5101 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5161 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5221 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5281 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5341 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5401 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5461 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5521 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5581 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5641 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5701 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5761 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5821 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5881 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5941 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6001 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6061 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6121 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6181 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6241 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6301 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6361 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6421 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6481 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6541 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6601 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6661 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6721 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6781 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6841 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     6901 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     6961 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7021 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7081 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7141 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7201 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7261 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7321 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7381 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7441 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7501 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7561 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7621 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7681 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7741 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7801 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7861 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7921 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7981 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8041 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccttgcg
     8101 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     8161 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     8221 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     8281 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     8341 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     8401 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     8461 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     8521 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     8581 cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga agaatccacc
     8641 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     8701 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     8761 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     8821 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     8881 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     8941 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     9001 ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt attcatccac
     9061 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     9121 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     9181 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg g
//
