LOCUS       sequence-462147-        6569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SUPV3L1/SUV3 (G207V) in mammalian cells with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Begeman A, Smolka JA, Shami A, Waingankar TP, Lewis SC
  TITLE     A spatial atlas of mitochondrial gene expression reveals dynamic 
            translation hubs and remodeling in stress.
  JOURNAL   bioRxiv [Preprint]. 2024 Aug 17:2024.08.05.604215. doi: 
            10.1101/2024.08.05.604215.
  PUBMED    39149346
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Aug 17:2024.08.05.604215. doi: 
            10.1101/2024.08.05.604215."
COMMENT     Sequence Label: SUPV3L1-HA G207V
FEATURES             Location/Qualifiers
     source          1..6569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(94..140)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    158..206
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        592..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     745..765
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3223..3249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3302..3423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3313..3332)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3367..3386
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      3538..3584
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3614..3750
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3795..3866
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(4092..4680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4172..4191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(5426..5494,5495..6286)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5644..5663)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      6299..6435
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      841 ctcgacacac ccgccagcgg ccgcatgtcc ttctcccgtg ccctattgtg ggctcggctc
      901 ccggcggggc gccaggctgg ccaccgggca gccatctgct ctgcccttcg tccccacttt
      961 gggccctttc ccggggttct ggggcaagtt tctgtccttg ccaccgcctc ctcctctgcc
     1021 tccggtggct ccaaaatacc aaacacgtcc ttgttcgtgc ccctgactgt gaaacctcag
     1081 ggccccagcg ccgacggcga cgtcggggcc gagctaaccc ggcctctgga caagaatgaa
     1141 gtaaagaagg tcttagacaa attttacaag aggaaagaaa ttcagaaact gggtgctgat
     1201 tatggacttg atgctcgtct cttccaccaa gctttcataa gctttagaaa ttatattatg
     1261 cagtctcatt ccctggatgt ggacattcac attgttttga atgatatttg cttcggtgca
     1321 gctcatgcgg atgatttatt cccatttttc ttgagacatg ccaaacaaat atttcctgtg
     1381 ttggactgta aggatgatct acgtaaaatc agtgacttaa gaataccacc taactggtac
     1441 ccagatgcta gagccatgca gcggaagata atatttcatt cagtccccac aaacagtgga
     1501 aagacttatc acgcaatcca gaaatacttc tcagcaaagt ctggagtgta ttgtggccct
     1561 ctaaaattac tggcacatga gatcttcgaa aagagtaatg ctgctggtgt gccatgtgac
     1621 ttggtgacag gtgaagagcg tgtgacagtt cagccaaatg ggaaacaggc ttcacatgtt
     1681 tcttgtacag ttgagatgtg cagtgttaca actccttatg aagtggctgt aattgatgaa
     1741 attcaaatga ttagagatcc agccagagga tgggcctgga ccagagcact tctaggactg
     1801 tgtgctgaag aggttcattt gtgtggagaa cctgctgcta ttgacctggt gatggagctt
     1861 atgtacacaa cgggggagga agtggaggtt cgagactata agaggcttac ccccatttct
     1921 gtgctggacc atgcactaga atctttagat aaccttcggc ctggggactg cattgtctgt
     1981 tttagcaaga atgatattta ttctgtgagt cggcagattg aaattcgggg attagaatca
     2041 gctgttatat atggcagtct cccacctggg accaaacttg ctcaagcaaa aaagtttaat
     2101 gatcccaatg acccatgcaa aatcttggtt gctacagatg caattggcat gggacttaat
     2161 ttgagcataa ggagaattat tttttactcc cttataaagc ccagtatcaa tgaaaaggga
     2221 gagagagaac tagaaccaat cacaacctct caagccctgc agattgctgg cagagctggc
     2281 agattcagct cacggtttaa agaaggagag gttacaacaa tgaatcatga agatctcagt
     2341 ttattaaagg aaattttgaa gaggcctgtg gatcctataa gggcagctgg tcttcatcca
     2401 actgctgagc agattgaaat gtttgcctac catctccctg atgcaacact gtccaatctc
     2461 attgatattt ttgtagactt ttcacaagtt gatgggcagt attttgtctg caatatggat
     2521 gattttaaat tttctgcaga gttgatccag catattccac taagtctgcg agtgaggtat
     2581 gttttctgca cagctcctat caacaagaag cagccttttg tgtgttcttc actgttacag
     2641 tttgccaggc agtatagcag gaatgagccc ctgacctttg catggttacg ccgatacatc
     2701 aaatggcctt tacttccacc taagaatatt aaagacctca tggatcttga agctgtccac
     2761 gatgtcttgg atctttactt gtggctaagc taccgattta tggatatgtt tccagatgcc
     2821 agccttattc gagatctcca gaaagaacta gatggtatta tccaagatgg tgtgcacaat
     2881 atcactaaat tgattaaaat gtctgagacg cataagctgt tgaatttgga gggctttcca
     2941 tcagggagcc agtcacgatt gtcaggaacc ttaaagagcc aagctagaag gacacgcggc
     3001 accaaagctc tagggagtaa agctactgag ccacccagcc ccgatgcagg agagctgtcc
     3061 cttgcttcca gattggtgca gcaaggactc ctcactccag acatgctgaa acagctagaa
     3121 aaagagtgga tgacacaaca aactgaacac aacaaagaaa aaacagagtc tgggactcat
     3181 ccaaaaggga cgagaagaaa gaagaaggaa cctgattcgg actacccata cgatgttcca
     3241 gattacgctt aggctagctt gactgactga gatacagcgt accttcagct cacagacatg
     3301 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3361 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3421 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3481 ttttaaagca agtaaaacct ctacaaatgt ggtattggcc catctctatc ggtatcgtag
     3541 cataacccct tggggcctct aaacgggtct tgaggggttt tttgtgcccc tcgggccgga
     3601 ttgctatcta ccggcattgg cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata
     3661 ctcccacata tgccagattc agcaacggat acggcttccc caacttgccc acttccatac
     3721 gtgtcctcct taccagaaat ttatccttaa ggtcgtcagc tatcctgcag gcgatctctc
     3781 gatttcgatc aagacattcc tttaatggtc ttttctggac accactaggg gtcagaagta
     3841 gttcatcaaa ctttcttccc tccctaatct cattggttac cttgggctat cgaaacttaa
     3901 ttaaccagtc aagtcagcta cttggcgaga tcgacttgtc tgggtttcga ctacgctcag
     3961 aattgcgtca gtcaagttcg atctggtcct tgctattgca cccgttctcc gattacgagt
     4021 ttcatttaaa tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4501 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4741 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4801 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4861 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcattt
     4921 aaatttccga actctccaag gccctcgtcg gaaaatcttc aaacctttcg tccgatccat
     4981 cttgcaggct acctctcgaa cgaactatcg caagtctctt ggccggcctt gcgccttggc
     5041 tattgcttgg cagcgcctat cgccaggtat tactccaatc ccgaatatcc gagatcggga
     5101 tcacccgaga gaagttcaac ctacatcctc aatcccgatc tatccgagat ccgaggaata
     5161 tcgaaatcgg ggcgcgcctg gtgtaccgag aacgatcctc tcagtgcgag tctcgacgat
     5221 ccatatcgtt gcttggcagt cagccagtcg gaatccagct tgggacccag gaagtccaat
     5281 cgtcagatat tgtactcaag cctggtcacg gcagcgtacc gatctgttta aacctagata
     5341 ttgatagtct gatcggtcaa cgtataatcg agtcctagct tttgcaaaca tctatcaaga
     5401 gacaggatca gcaggaggct ttcgcatgag tattcaacat ttccgtgtcg cccttattcc
     5461 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5521 agatgctgaa gatcagttgg gtgcgcgagt gggttacatc gaactggatc tcaacagcgg
     5581 taagatcctt gagagttttc gccccgaaga acgctttcca atgatgagca cttttaaagt
     5641 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5701 catacactat tctcagaatg acttggttga gtattcacca gtcacagaaa agcatcttac
     5761 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5821 ggccaactta cttctgacaa cgattggagg accgaaggag ctaaccgctt ttttgcacaa
     5881 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5941 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaaccttgc gtaaactatt
     6001 aactggcgaa ctacttactc tagcttcccg gcaacagttg atagactgga tggaggcgga
     6061 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6121 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6181 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6241 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaaccga ttctaggtgc
     6301 attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca
     6361 gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca
     6421 gaaatttatc cttaagatcc cgaatcgttt aaactcgact ctggctctat cgaatctccg
     6481 tcgtttcgag cttacgcgaa cagccgtggc gctcatttgc tcgtcgggca tcgaatctcg
     6541 tcagctatcg tcagcttacc tttttggca
//
