LOCUS       sequence-46216-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, 
            Bosak S, Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.
  TITLE     A first version of the Caenorhabditis elegans Promoterome.
  JOURNAL   Genome Res. 2004 Oct;14(10B):2169-75.
  PUBMED    15489340
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2004-10"; volume: "14(10B)"; pages: "2169-75"
COMMENT     Sequence Label: pdestDD03
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    70..193
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(234..539)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(336..355)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agctcgaaat taaccctcac taaagggaac aaaagctggt accggtccgg aattcccggg
       61 tcgagatatc aactttgtac aagaaagttg aacgagaaac gtaaaatgat ataaatatca
      121 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
      181 tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt
      241 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
      301 acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag
      361 ctttcatccc cgatatgcac caccgggtaa agttcacggg ggactttatc tgacagcaga
      421 cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt
      481 acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt
      541 tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcattaaacc gggcgacctc
      601 agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt
      661 ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga
      721 tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca
      781 tactttcggg tatacatatc agtatatatt cttataccgc aaaatcagcg cgcaaatacg
      841 catactgtta tctggctttt agtaagccgg atcctctaga ttacgccccg cctgccactc
      901 atcgcagtac tgtggtaatt catta
//
