LOCUS       sequence-462166-       12213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stably express a constitutively stable version of HIF2a (HIF2a dPA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   Jiang Q, Braun DA, Clauser KR, Ramesh V, Shirole NH, Duke-Cohan JE, 
            Nabilsi N, Kramer NJ, Forman C, Lippincott IE, Klaeger S, Phulphagar
            KM, Chea V, Kim N, Vanasse AP, Saad E, Parsons T, Carr-Reynolds M, 
            Carulli I, Pinjusic K, Jiang Y, Li R, Syamala S, Rachimi S, Verzani 
            EK, Stevens JD, Lane WJ, Camp SY, Meli K, Pappalardi MB, Herbert ZT,
            Qiu X, Cejas P, Long HW, Shukla SA, Van Allen EM, Choueiri TK, 
            Churchman LS, Abelin JG, Gurer C, MacBeath G, Childs RW, Carr SA, 
            Keskin DB, Wu CJ, Kaelin WG Jr
  TITLE     HIF regulates multiple translated endogenous retroviruses: 
            Implications for cancer immunotherapy.
  JOURNAL   Cell. 2025 Feb 25:S0092-8674(25)00156-4. doi: 
            10.1016/j.cell.2025.01.046.
  PUBMED    40023154
REFERENCE   2  (bases 1 to 12213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Feb 25:S0092-8674(25)00156-4. doi: 10.1016/j.cell.2025.01.046."
COMMENT     Sequence Label: pLenti-EF1a-HIF2a dPA (P405A, P531A)
FEATURES             Location/Qualifiers
     source          1..12213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1069..1267
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1222..1242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2134..2367
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2552..2596
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2894..3011
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3083..4261
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3314..4252
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4209..4229
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4278..4296
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4370..4394
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7028..7052)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7099..7188
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        7230..7729
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7265..7288)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7662..7681
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7750..8349
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7750..7769)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8246..8266
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(8380..8413)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8469..9057
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8522..8542)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8940..8951)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9060..9076)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9061..9077)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9266..9499
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9571..9692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9608..9627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9662..9681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9732..9867
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9888..9906)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(9929..10287)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9969..9990)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10181..10200
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10314..10418
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10420..10777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10420..10611
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10443..10463)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10628..10763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10812..11606
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10812..11606
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10866..10885)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11476..11495
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11794..11813)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11838..11885
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     2041 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     2101 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     2161 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     2221 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     2281 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     2341 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     2401 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     2461 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     2521 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2581 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2641 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2701 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2761 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2821 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     2881 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2941 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     3001 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgggcc
     3061 cgctctagcc ctagatttaa atggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3121 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     3181 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3241 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3301 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     3361 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
     3421 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     3481 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     3541 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     3601 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     3661 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     3721 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     3781 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     3841 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     3901 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     3961 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     4021 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     4081 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     4141 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     4201 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     4261 aggaattagc ttggtactaa tacgactcac tatagggaga cccaagctgg ctagccgcgg
     4321 aatctagact gccggatcga attgtcgacg aattcggatc tggccaatca caagtttgta
     4381 caaaaaagca ggctcttccc tcggatccgc caccatgaca gctgacaagg agaagaaaag
     4441 gagtagctcg gagaggagga aggagaagtc ccgggatgct gcgcggtgcc ggcggagcaa
     4501 ggagacggag gtgttctatg agctggccca tgagctgcct ctgccccaca gtgtgagctc
     4561 ccatctggac aaggcctcca tcatgcgact ggcaatcagc ttcctgcgaa cacacaagct
     4621 cctctcctca gtttgctctg aaaacgagtc cgaagccgaa gctgaccagc agatggacaa
     4681 cttgtacctg aaagccttgg agggtttcat tgccgtggtg acccaagatg gcgacatgat
     4741 ctttctgtca gaaaacatca gcaagttcat gggacttaca caggtggagc taacaggaca
     4801 tagtatcttt gacttcactc atccctgcga ccatgaggag attcgtgaga acctgagtct
     4861 caaaaatggc tctggttttg ggaaaaaaag caaagacatg tccacagagc gggacttctt
     4921 catgaggatg aagtgcacgg tcaccaacag aggccgtact gtcaacctca agtcagccac
     4981 ctggaaggtc ttgcactgca cgggccaggt gaaagtctac aacaactgcc ctcctcacaa
     5041 tagtctgtgt ggctacaagg agcccctgct gtcctgcctc atcatcatgt gtgaaccaat
     5101 ccagcaccca tcccacatgg acatccccct ggatagcaag accttcctga gccgccacag
     5161 catggacatg aagttcacct actgtgatga cagaatcaca gaactgattg gttaccaccc
     5221 tgaggagctg cttggccgct cagcctatga attctaccat gcgctagact ccgagaacat
     5281 gaccaagagt caccagaact tgtgcaccaa gggtcaggta gtaagtggcc agtaccggat
     5341 gctcgcaaag catgggggct acgtgtggct ggagacccag gggacggtca tctacaaccc
     5401 tcgcaacctg cagccccagt gcatcatgtg tgtcaactac gtcctgagtg agattgagaa
     5461 gaatgacgtg gtgttctcca tggaccagac tgaatccctg ttcaagcccc acctgatggc
     5521 catgaacagc atctttgata gcagtggcaa gggggctgtg tctgagaaga gtaacttcct
     5581 attcaccaag ctaaaggagg agcccgagga gctggcccag ctggctgcta ccccaggaga
     5641 cgccatcatc tctctggatt tcgggaatca gaacttcgag gagtcctcag cctatggcaa
     5701 ggccatcctg cccccgagcc agccatgggc cacggagttg aggagccaca gcacccagag
     5761 cgaggctggg agcctgcctg ccttcaccgt gccccaggca gctgccccgg gcagcaccac
     5821 ccccagtgcc accagcagca gcagcagctg ctccacgccc aatagccctg aagactatta
     5881 cacatctttg gataacgacc tgaagattga agtgattgag aagctcttcg ccatggacac
     5941 agaggccaag gaccaatgca gtacccagac ggatttcaat gagctggact tggagacact
     6001 ggcagcttat atccccatgg acggggaaga cttccagcta agccccatct gccccgagga
     6061 gcggctcttg gcggagaacc cacagtccac cccccagcac tgcttcagtg ccatgacaaa
     6121 catcttccag ccactggccc ctgtagcccc gcacagtccc ttcctcctgg acaagtttca
     6181 gcagcagctg gagagcaaga agacagagcc cgagcaccgg cccatgtcct ccatcttctt
     6241 tgatgccgga agcaaagcat ccctgccacc gtgctgtggc caggccagca cccctctctc
     6301 ttccatgggg ggcagatcca atacccagtg gcccccagat ccaccattac attttgggcc
     6361 cacaaagtgg gccgtcgggg atcagcgcac agagttcttg ggagcagcgc cgttggggcc
     6421 ccctgtctct ccaccccatg tctccacctt caagacaagg tctgcaaagg gttttggggc
     6481 tcgaggccca gacgtgctga gtccggccat ggtagccctc tccaacaagc tgaagctgaa
     6541 gcgacagctg gagtatgaag agcaagcctt ccaggacctg agcggggggg acccacctgg
     6601 tggcagcacc tcacatttga tgtggaaacg gatgaagaac ctcaggggtg ggagctgccc
     6661 tttgatgccg gacaagccac tgagcgcaaa tgtacccaat gataagttca cccaaaaccc
     6721 catgaggggc ctgggccatc ccctgagaca tctgccgctg ccacagcctc catctgccat
     6781 cagtcccggg gagaacagca agagcaggtt ccccccacag tgctacgcca cccagtacca
     6841 ggactacagc ctgtcgtcag cccacaaggt gtcaggcatg gcaagccggc tgctcgggcc
     6901 ctcatttgag tcctacctgc tgcccgaact gaccagatat gactgtgagg tgaacgtgcc
     6961 cgtgctggga agctccacgc tcctgcaagg aggggacctc ctcagagccc tggaccaggc
     7021 cacctgaacc cagctttctt gtacaaagtg gtgggtggag gcggttcagg tggcggagga
     7081 agcggaggcg gtggaagtta cccctacgac gtgcccgact acgccggcta tccgtatgat
     7141 gtcccggact atgcaggatc ctatccatac gacgttccag attacgctta gttaattaag
     7201 gccggccctc gaggttaacg aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     7261 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     7321 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     7381 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     7441 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     7501 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     7561 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     7621 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     7681 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     7741 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     7801 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     7861 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     7921 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     7981 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     8041 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     8101 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     8161 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     8221 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     8281 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     8341 ggtgcctgat gtacaagtag gaattcgtcg agggacctaa taacttcgta tagcatacat
     8401 tatacgaagt tatacatgtt taagggttcc ggttccacta ggtacaattc gatatcaagc
     8461 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     8521 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     8581 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     8641 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     8701 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     8761 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     8821 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     8881 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     8941 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     9001 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     9061 gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca
     9121 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     9181 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     9241 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     9301 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     9361 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     9421 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     9481 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     9541 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     9601 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9661 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     9721 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9781 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     9841 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     9901 gtattagcat gtgagcaaaa ggccagcata aatcaaaaga atagaccgag atagggttga
     9961 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
    10021 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
    10081 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
    10141 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
    10201 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
    10261 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
    10321 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10381 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
    10441 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    10501 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    10561 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    10621 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10681 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    10741 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
    10801 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
    10861 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
    10921 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
    10981 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
    11041 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
    11101 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
    11161 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
    11221 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
    11281 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
    11341 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
    11401 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
    11461 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
    11521 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
    11581 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
    11641 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    11701 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    11761 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
    11821 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
    11881 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
    11941 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
    12001 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
    12061 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    12121 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    12181 gtcagacccc gtagaaaaga tcaaaggatc ttc
//
