LOCUS       sequence-46217-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, 
            Bosak S, Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.
  TITLE     A first version of the Caenorhabditis elegans Promoterome.
  JOURNAL   Genome Res. 2004 Oct;14(10B):2169-75.
  PUBMED    15489340
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2004-10"; volume: "14(10B)"; pages: "2169-75"
COMMENT     Sequence Label: pdestMB14
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..146
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(187..492)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(289..308)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctcatttttt ctaccggtac atcaactttg tacaagaaag ttgaacgaga aacgtaaaat
       61 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
      121 aaaacacaac atatccagtc actatggtcg acctgcagac tggctgtgta taagggagcc
      181 tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg
      241 gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt
      301 catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac gggggacttt
      361 atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat
      421 aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac
      481 cttaaactgc atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa
      541 accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac
      601 gacgggcttc attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc
      661 cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata
      721 cctctttttg acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca
      781 gcgcgcaaaa tacgcatact gttatctggc tttttagtat gccggatcct ctagattacg
      841 ccccgcctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      901 gccatcacaa acggcatgat gaacctgaat cgccagcgg
//
