LOCUS       sequence-462172-        9464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 
            virus-like particles (VLPs).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Yang Q, Davidson BA, Pajic P, Chen X, Gokcumen O, Gao M, Neelamegham
            S
  TITLE     Tuning the tropism and infectivity of SARS-CoV-2 virus-like 
            particles for mRNA delivery.
  JOURNAL   Nucleic Acids Res. 2025 Feb 27;53(5):gkaf133. doi: 
            10.1093/nar/gkaf133.
  PUBMED    40037714
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-02-27"; volume: "53(5):gkaf133. doi"; 
            pages: " 10.1093/nar/gkaf133"
COMMENT     Sequence Label: Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9
FEATURES             Location/Qualifiers
     source          1..9464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        185..564
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        565..768
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     719..739
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     813..832
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        813..831
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      892..901
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(898..900,901..903,904..1614)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(898..900,901..903,904..1614)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(943..964)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1204..1223)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1551..1572
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1630..1686
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1693..2394
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(1826..1844)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2094..2113
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2288..2311
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             2413..2466
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2467..3168
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    4348..4936
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4401..4421)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4819..4830)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5005..5022)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5011..5235
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5281..5709
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5368..5387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5578..5599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5718..5738)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5723..6052
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5903..6038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5965..5984
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6119..6913
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6173..6192)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6783..6802
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7087..7208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7124..7143)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7178..7197
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7257..7273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7257..7273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7270..7292)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7281..7297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7305..7311,7312..7329,7330..7335))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7350..7371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7488..7505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7659..8247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7739..7758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8418..9209,9210..9278))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9041..9060
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9279..9383)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(9458..9464,1..13))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
       61 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
      121 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
      181 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      241 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      301 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      361 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      421 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      481 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      541 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      601 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      661 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      721 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      781 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      841 gctggctagc ttgttagacg aagcttgggc tgcaggtccg atccaccggt cgccaccatg
      901 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      961 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1021 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1081 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1141 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1201 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1261 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1321 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1381 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1441 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1501 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1561 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggaagc
     1621 ggcagcggcg ccaccaactt cagcctgctg aagcaggccg gcgacgtgga ggagaacccc
     1681 ggccccgtta acatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
     1741 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     1801 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     1861 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     1921 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     1981 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     2041 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     2101 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     2161 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     2221 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     2281 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     2341 cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta caagggcggc
     2401 gggtccggag gagagggcag aggaagtctc ctaacatgcg gtgacgtgga ggagaatcct
     2461 ggcccaatga gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
     2521 gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
     2581 cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
     2641 gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac
     2701 ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaggac
     2761 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
     2821 gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc
     2881 ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac
     2941 gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat
     3001 agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga
     3061 ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg
     3121 gccagatact gcgacctccc tagcaaactg gggcacaagc ttaattgatg cggccgcctc
     3181 ctttaagaag gagatataga tctctgtagg tcccaaacaa gctagtctta atggagtcac
     3241 attaattgga gaagccgtaa aaacacagtt caattattat aagaaagttg atggtgttgt
     3301 ccaacaatta cctgaaactt actttactca gagtagaaat ttacaagaat ttaaacccag
     3361 gagtcaaatg gaaattgatt tcttagaatt agctatggat gaattcattg aacggtataa
     3421 attagaaggc tatgccttcg aacatatcgt ttatggagat tttagtcata gtcagttagg
     3481 tggtttacat ctactgattg gactagctaa acgttttaag gaatcacctt ttgaattaga
     3541 agattttatt cctatggaca gtacagttaa aaactatttc ataacagatg cgcaaacagg
     3601 ttcatctaag tgtgtgtgtt ctgttattga tttattactt gatgattttg ttgaaataat
     3661 aaaatcccaa gatttatctg tagtttctaa ggttgtcaaa gtgactattg actatacaga
     3721 aatttcattt atgctttggt gtaaagatgg ccatgtagaa acattttacc caaaattaca
     3781 atctagtcaa gcgtggcaac cgggtgttgc tatgcctaat ctttacaaaa tgcaaagaat
     3841 gctattagaa aagtgtgacc ttcaaaatta tggtgatagt gcaacattac ctaaaggcat
     3901 aatgatgaat gtcgcaaaat atactcaact gtgtcaatat ttaaacacat taacattagc
     3961 tgtaccctat aatatgagag ttatacattt tggtgctggt tctgataaag gagttgcacc
     4021 aggtacagct gttttaagac agtggttgcc tacgggtacg ctgcttgtcg attcagatct
     4081 taatgacttt gtctctgatg cagattcaac tttgattggt gattgtgcaa ctgtacatac
     4141 agctaataaa tgggatctca ttattagtga tatgtacgac cctaagacta aaaatgttac
     4201 aaaagaaaat gactctaaag agggtttttt cacttacatt tgtgggttta tacaacaaaa
     4261 gctagctctt ggaggttccg tggctataaa gatagatccc aactccataa gtgtgtcgac
     4321 atataaggat ccactcgacg actcgacaat caacctctgg attacaaaat ttgtgaaaga
     4381 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4441 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4501 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4561 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4621 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4681 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4741 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4801 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4861 ccggctctgc ggcctcttcc gcatcttcgc cttcgccctc agacgagtcg gatctccctt
     4921 tgggccgcct ccccgcctgg aattaattcg agctcggtac cttgattctc gagtctagag
     4981 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5041 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5101 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5161 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     5221 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     5281 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5341 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5401 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5461 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5521 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5581 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5641 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5701 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5761 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5821 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5881 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5941 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     6001 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6061 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     6121 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6181 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6241 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6301 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6361 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6421 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     6481 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     6541 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     6601 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     6661 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6721 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     6781 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     6841 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     6901 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     6961 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7021 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     7081 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7141 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7201 gtatcttatc atgtctgtat accctccacc tctagctaga gcttggcgta atcatggtca
     7261 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7321 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7381 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7441 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7501 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7561 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8341 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8401 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8461 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8521 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8581 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8641 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8701 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8761 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8821 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8881 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8941 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9001 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9061 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9121 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9181 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9241 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9301 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9361 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cctccacgga
     9421 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatc
//
