LOCUS       sequence-462179-       12811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a dexamethasone (DEX)-inducible 
            promoter driving in frame epitope fusion of eGFP paired with ccdb 
            selection toxin and mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12811)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 12811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DEXsynth:ccdb-eGFP + mcherry
FEATURES             Location/Qualifiers
     source          1..12811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     510..529
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(929..934,935..951,952..957))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1250..1444
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1510..2583)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3012..3641)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3905..3929
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5181..5414
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        7025..7071
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    7115..7239
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7264..7269,7270..7287,7288..7294)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7348..8007
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7414..7433)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8349..8654
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8533..8552
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8695..8819)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(8827..8829,8830..8832,8833..9546)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8827..8829,8830..8832,8833..9543)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8872..8893)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9133..9152)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9480..9501
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10349..11059
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     10762..10781
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      11068..11320
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11375..11399
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(11522..12110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11602..11621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12502..12811,1..881))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
       61 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
      121 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
      181 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
      241 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
      301 aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
      361 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
      421 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
      481 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
      541 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg
      601 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
      661 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
      721 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
      781 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      841 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
      901 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
      961 tgagaattct tgaaaatatt gcgctgccat ttttggggtg aggccgttcg cggccgaggg
     1021 gcgcagcccc tggggggatg ggagctcggt gccttgatgt gggcgccggc ggtcgagtgg
     1081 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     1141 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     1201 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     1261 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     1321 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     1381 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     1441 cacaggaaag aagctttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     1501 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     1561 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     1621 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
     1681 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
     1741 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
     1801 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
     1861 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
     1921 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
     1981 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
     2041 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
     2101 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
     2161 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
     2221 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
     2281 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
     2341 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg acgcaccggc
     2401 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
     2461 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
     2521 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
     2581 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
     2641 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
     2701 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
     2761 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
     2821 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
     2881 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
     2941 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
     3001 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
     3061 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
     3121 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
     3181 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
     3241 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
     3301 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
     3361 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
     3421 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
     3481 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
     3541 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
     3601 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
     3661 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
     3721 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
     3781 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggctgatg
     3841 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     3901 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     3961 tgcggacgtt tttaatgtac tggggtggat gcagtgggcc ccactctgtg gaagacaatg
     4021 cctccggagt aacagataaa gcgaagcttg aaggtgcatg tgactccgtc aagattacga
     4081 aaccgccaac taccacgcaa attgcaattc tcaatttcct agaaggactc tccgaaaatg
     4141 catccaatac caaatattac ccgtgtcata ggcaccaagt gacaccatac atgaacacgc
     4201 gtcacaatat gactggagaa gggttccaca ccttatgcta taaaacgccc cacacccctc
     4261 ctccttcctt cgcagttcaa ttccaatata ttccattctc tctgtgtatt tccctacctc
     4321 tcccttcaag gttagtcgat ttcttctgtt tttcttcttc gttctttcca tgaattgtgt
     4381 atgttctttg atcaatacga tgttgatttg attgtgtttt gtttggtttc atcgatcttc
     4441 aattttcata atcagattca gcttttatta tctttacaac aacgtcctta atttgatgat
     4501 tctttaatcg tagatttgct ctaattagag ctttttcatg tcagatccct ttacaacaag
     4561 ccttaattgt tgattcatta atcgtagatt agggcttttt tcattgatta cttcagatcc
     4621 gttaaacgta accatagatc agggcttttt catgaattac ttcagatccg ttaaacaaca
     4681 gccttatttt ttatacttct gtggtttttc aagaaattgt tcagatccgt tgacaaaaag
     4741 ccttattcgt tgattctata tcgtttttcg agagatattg ctcagatctg ttagcaactg
     4801 ccttgtttgt tgattctatt gccgtggatt agggtttttt ttcacgagat tgcttcagat
     4861 ccgtacttaa gattacgtaa tggattttga ttctgattta tctgtgattg ttgactcgac
     4921 agcggccgca ccggcgcgcc atggcagatc caatgaagct actgtcttct atcgaacaag
     4981 catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca
     5041 agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaaga tctccgctga
     5101 ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac
     5161 tgatttttcc aaggtcgagc gcccccccga ccgatgtcag cctgggggac gagctccact
     5221 tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc gatctggaca
     5281 tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc gccccctacg
     5341 gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc cttggaattg
     5401 acgagtacgg tggggatcca attcagcaag ccactgcagg agtctcacaa gacacttcgg
     5461 aaaatcctaa caaaacaata gttcctgctg cattaccaca gctcacccct accttggtgt
     5521 cactgctgga ggtgattgaa cccgaggtgt tgtatgcagg atatgatagc tctgttccag
     5581 attcagcatg gagaattatg accacactca acatgttagg tgggcgtcaa gtgattgcag
     5641 cagtgaaatg ggcaaaggcg ataccaggct tcagaaactt acacctggat gaccaaatga
     5701 ccctgctaca gtactcatgg atgtttctca tggcatttgc cctgggttgg agatcataca
     5761 gacaatcaag tggaaacctg ctctgctttg ctcctgatct gattattaat gagcagagaa
     5821 tgtctctacc ctgcatgtat gaccaatgta aacacatgct gtttgtctcc tctgaattac
     5881 aaagattgca ggtatcctat gaagagtatc tctgtatgaa aaccttactg cttctctcct
     5941 cagttcctaa ggaaggtctg aagagccaag agttatttga tgagattcga atgacttata
     6001 tcaaagagct aggaaaagcc atcgtcaaaa gggaagggaa ctccagtcag aactggcaac
     6061 ggttttacca actgacaaag cttctggact ccatgcatga ggtggttgag aatctcctta
     6121 cctactgctt ccagacattt ttggataaga ccatgagtat tgaattccca gagatgttag
     6181 ctgaaatcat cactaatcag ataccaaaat attcaaatgg aaatatcaaa aagcttctgt
     6241 ttcatcaaaa atgactcgac ctaactgagt aagctagctt gttcgagtat tatggcattg
     6301 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg
     6361 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc
     6421 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat
     6481 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc
     6541 ctctaatgac cgaagttaat atgaggagta aaacactaga tccccaaaca agcttgaaac
     6601 tgaaggcggg aaacgacaat ctgatcatga gcggagaatt aagggagtca cgttatgacc
     6661 cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacatatga aaatattttt
     6721 taatgcattt tatgacttgc caattgattg acaacatgca tcaatccgcg gttatgactc
     6781 tcttaagaga gtcatgaatt cgagcgggaa ttgatccccc ctcgacagct tgcatgccgg
     6841 tcgactctag aggatccggg tgacagccct ccgacgggtg acagccctcc gacgggtgac
     6901 agccctccga attctagagg atccgggtga cagccctccg acgggtgaca gccctccgac
     6961 gggtgacagc cctccgaatt cgagctcagt acccggggat ctgtcgacct cgatcgagat
     7021 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa
     7081 gctagtcgac tctagcctcg aatccttagg taccacaagt ttgtacaaaa aagctgaacg
     7141 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     7201 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca
     7261 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
     7321 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     7381 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     7441 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     7501 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     7561 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     7621 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     7681 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     7741 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     7801 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     7861 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     7921 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     7981 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     8041 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     8101 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     8161 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     8221 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     8281 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     8341 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     8401 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     8461 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     8521 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     8581 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     8641 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     8701 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     8761 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg
     8821 atatcaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     8881 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     8941 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     9001 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     9061 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     9121 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     9181 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     9241 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     9301 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     9361 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     9421 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     9481 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     9541 aagtaaccta ggttcggttc gagtattatg gcattgggaa aactgttttt cttgtaccat
     9601 ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg
     9661 gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct caaattaata
     9721 ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga gatatgcaaa
     9781 cattttgttt tgagtaaaaa tgtgtcaaat caaggttcca gaaggtaatt atccaagatg
     9841 tagcatcaag aatccaatgt ttacgggaaa aactatggaa gtattatgtg agctcagcaa
     9901 gaagcagatc aatatgcggc acatatgcaa cctatgttca aaaatgaaga atgtacagat
     9961 acaagatcct atactgccag aatacgaaga agaatacgta gaaattgaaa aagaagaacc
    10021 aggcgaagaa aagaatcttg aagacgtaag cactgacgac aacaatgaaa agaagaagat
    10081 aaggtcggtg attgtgaaag agacatagag gacacatgta aggtggaaaa tgtaagggcg
    10141 gaaagtaacc ttatcacaaa ggaatcttat cccccactac ttatcctttt atatttttcc
    10201 gtgtcatttt tgcccttgag ttttcctata taaggaacca agttcggcat ttgtgaaaac
    10261 aagaaaaaat ttggtgtaag ctattttctt tgaagtactg aggatacaac ttcagagaaa
    10321 tttgtaagtt tgtgatccgc aaacaaaaat ggtgagcaag ggcgaggagg ataacatggc
    10381 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
    10441 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
    10501 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
    10561 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
    10621 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
    10681 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
    10741 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
    10801 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
    10861 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
    10921 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
    10981 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
    11041 ggacgagctg tacaagtgag atgtgcagat cgttcaaaca tttggcaata aagtttctta
    11101 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
    11161 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
    11221 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
    11281 gataaattat cgcgcgcggt gtcatctatg ttactagatc cgatgataag ctgtcaaaca
    11341 tgagggacac gaagtggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
    11401 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
    11461 ccgttcgtcc atttgtatgt gccaaccaca ggaaccgtaa aaaggccgcg ttgctggcgt
    11521 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    11581 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    11641 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    11701 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    11761 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    11821 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    11881 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    11941 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    12001 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    12061 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    12121 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    12181 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    12241 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctaaaacaat tcatccagta
    12301 aaatataatc gaatattatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc
    12361 gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg
    12421 cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag
    12481 cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa
    12541 cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt
    12601 gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct
    12661 ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
    12721 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
    12781 gaaggccatc cagcctcgcg tcgcgaacgc c
//
