LOCUS       sequence-462180-       10079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter 
            driving in frame epitope fusion of 3xHA tag paired with ccdb 
            selection toxin and mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10079)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Ubi:3xHA-ccdb + mcherry
FEATURES             Location/Qualifiers
     source          1..10079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             718..1023
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     902..921
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1064..1188)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1991..2701
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2404..2423
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      2710..2962
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3017..3041
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3164..3752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3244..3263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4144..5334)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     4963..4982
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(5382..5387,5388..5404,5405..5410))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      5703..5897
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5963..7036)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7465..8094)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8358..8382
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9440..9529
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    9551..9675
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(9712..9717,9718..9735,9736..9742)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             join(9796..10079,1..376)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9862..9881)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
       61 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
      121 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
      181 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
      241 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
      301 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
      361 gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta
      421 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
      481 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
      541 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
      601 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
      661 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
      721 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
      781 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
      841 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
      901 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
      961 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     1021 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     1081 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     1141 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtcc taggttcggt
     1201 tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt
     1261 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag
     1321 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt
     1381 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa
     1441 aatgtgtcaa atcaaggttc cagaaggtaa ttatccaaga tgtagcatca agaatccaat
     1501 gtttacggga aaaactatgg aagtattatg tgagctcagc aagaagcaga tcaatatgcg
     1561 gcacatatgc aacctatgtt caaaaatgaa gaatgtacag atacaagatc ctatactgcc
     1621 agaatacgaa gaagaatacg tagaaattga aaaagaagaa ccaggcgaag aaaagaatct
     1681 tgaagacgta agcactgacg acaacaatga aaagaagaag ataaggtcgg tgattgtgaa
     1741 agagacatag aggacacatg taaggtggaa aatgtaaggg cggaaagtaa ccttatcaca
     1801 aaggaatctt atcccccact acttatcctt ttatattttt ccgtgtcatt tttgcccttg
     1861 agttttccta tataaggaac caagttcggc atttgtgaaa acaagaaaaa atttggtgta
     1921 agctattttc tttgaagtac tgaggataca acttcagaga aatttgtaag tttgtgatcc
     1981 gcaaacaaaa atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     2041 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     2101 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     2161 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     2221 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     2281 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     2341 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     2401 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     2461 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     2521 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     2581 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     2641 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtg
     2701 agatgtgcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     2761 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     2821 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     2881 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     2941 gtgtcatcta tgttactaga tccgatgata agctgtcaaa catgagggac acgaagtggt
     3001 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     3061 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     3121 gtgccaacca caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3601 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3901 atgagtaaac ttggtctgac agctaaaaca attcatccag taaaatataa tcgaatatta
     3961 tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga
     4021 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca
     4081 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc
     4141 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
     4201 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata
     4261 aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga
     4321 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     4381 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     4441 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc
     4501 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
     4561 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
     4621 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     4681 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     4741 gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa
     4801 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     4861 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     4921 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     4981 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     5041 gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     5101 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     5161 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
     5221 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
     5281 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg
     5341 tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat
     5401 aagctgtcaa acatgagaat tcttgaaaat attgcgctgc catttttggg gtgaggccgt
     5461 tcgcggccga ggggcgcagc ccctgggggg atgggagctc ggtgccttga tgtgggcgcc
     5521 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     5581 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     5641 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     5701 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     5761 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     5821 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     5881 tgtacgtgct atccacagga aagaagcttt ttcgaccttt ttcccctgct agggcaattt
     5941 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     6001 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     6061 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     6121 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     6181 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     6241 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     6301 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     6361 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     6421 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     6481 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     6541 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     6601 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     6661 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     6721 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     6781 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     6841 acgacgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     6901 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     6961 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     7021 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
     7081 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
     7141 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
     7201 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
     7261 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
     7321 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
     7381 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
     7441 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
     7501 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
     7561 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
     7621 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
     7681 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
     7741 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
     7801 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
     7861 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
     7921 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
     7981 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
     8041 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
     8101 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
     8161 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
     8221 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
     8281 cgcacggctg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
     8341 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
     8401 cttaataaca cattgcggac gtttttaatg tactggggtg gatgcagtgg gccccactct
     8461 gtggaagaca atgccccgcg gtccggagta acagataaag cgaagcttga aggtgcatgt
     8521 gactccgtca agattacgaa accgccaact accacgcaaa ttgcaattct caatttccta
     8581 gaaggactct ccgaaaatgc atccaatacc aaatattacc cgtgtcatag gcaccaagtg
     8641 acaccataca tgaacacgcg tcacaatatg actggagaag ggttccacac cttatgctat
     8701 aaaacgcccc acacccctcc tccttccttc gcagttcaat tccaatatat tccattctct
     8761 ctgtgtattt ccctacctct cccttcaagg ttagtcgatt tcttctgttt ttcttcttcg
     8821 ttctttccat gaattgtgta tgttctttga tcaatacgat gttgatttga ttgtgttttg
     8881 tttggtttca tcgatcttca attttcataa tcagattcag cttttattat ctttacaaca
     8941 acgtccttaa tttgatgatt ctttaatcgt agatttgctc taattagagc tttttcatgt
     9001 cagatccctt tacaacaagc cttaattgtt gattcattaa tcgtagatta gggctttttt
     9061 cattgattac ttcagatccg ttaaacgtaa ccatagatca gggctttttc atgaattact
     9121 tcagatccgt taaacaacag ccttattttt tatacttctg tggtttttca agaaattgtt
     9181 cagatccgtt gacaaaaagc cttattcgtt gattctatat cgtttttcga gagatattgc
     9241 tcagatctgt tagcaactgc cttgtttgtt gattctattg ccgtggatta gggttttttt
     9301 tcacgagatt gcttcagatc cgtacttaag attacgtaat ggattttgat tctgatttat
     9361 ctgtgattgt tgactcgaca gcggccgcac ccccgggggc gcgcccttag gtaccatgag
     9421 cgggttaatt aacatctttt acccatacga tgttcctgac tatgcgggct atccctatga
     9481 cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcag
     9541 cgctgttatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     9601 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     9661 tatccagtca ctatggcggc ccctcgaggg gccgcattag gcaccccagg ctttacactt
     9721 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
     9781 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     9841 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     9901 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     9961 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
    10021 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgtttt
//
