LOCUS       sequence-462181-       12807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a dexamethasone (DEX)-inducible 
            promoter driving in frame epitope fusion of eGFP paired with ccdb 
            selection toxin and mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12807)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 12807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DEXsynth:eGFP-ccdb + mcherry
FEATURES             Location/Qualifiers
     source          1..12807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(879..1508)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    1772..1796
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3048..3281
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        4892..4938
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(4982..4984,4985..4987,4988..5698)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4982..4984,4985..4987,4988..5698)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5027..5048)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5288..5307)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5635..5656
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5705..5829
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5854..5859,5860..5877,5878..5884)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5938..6597
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6004..6023)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6939..7244
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7123..7142
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7285..7409)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8212..8922
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     8625..8644
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      8931..9183
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9238..9262
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9385..9973)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9465..9484)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10365..11555)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     11184..11203
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(11603..11608,11609..11625,11626..11631))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      11924..12118
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(12184..12807,1..450))
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
       61 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
      121 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
      181 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
      241 cggcttccta atcgacgacg caccggctgc cggcggttgc cgggattctt tgcggattcg
      301 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
      361 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
      421 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
      481 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
      541 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
      601 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
      661 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
      721 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
      781 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
      841 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
      901 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
      961 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
     1021 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
     1081 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
     1141 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
     1201 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
     1261 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
     1321 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
     1381 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
     1441 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
     1501 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
     1561 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
     1621 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
     1681 ggatttcatg cagccgcacg gctgatgggc tgcctgtatc gagtggtgat tttgtgccga
     1741 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     1801 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggatgca
     1861 gtgggcccca ctctgtggaa gacaatgcct ccggagtaac agataaagcg aagcttgaag
     1921 gtgcatgtga ctccgtcaag attacgaaac cgccaactac cacgcaaatt gcaattctca
     1981 atttcctaga aggactctcc gaaaatgcat ccaataccaa atattacccg tgtcataggc
     2041 accaagtgac accatacatg aacacgcgtc acaatatgac tggagaaggg ttccacacct
     2101 tatgctataa aacgccccac acccctcctc cttccttcgc agttcaattc caatatattc
     2161 cattctctct gtgtatttcc ctacctctcc cttcaaggtt agtcgatttc ttctgttttt
     2221 cttcttcgtt ctttccatga attgtgtatg ttctttgatc aatacgatgt tgatttgatt
     2281 gtgttttgtt tggtttcatc gatcttcaat tttcataatc agattcagct tttattatct
     2341 ttacaacaac gtccttaatt tgatgattct ttaatcgtag atttgctcta attagagctt
     2401 tttcatgtca gatcccttta caacaagcct taattgttga ttcattaatc gtagattagg
     2461 gcttttttca ttgattactt cagatccgtt aaacgtaacc atagatcagg gctttttcat
     2521 gaattacttc agatccgtta aacaacagcc ttatttttta tacttctgtg gtttttcaag
     2581 aaattgttca gatccgttga caaaaagcct tattcgttga ttctatatcg tttttcgaga
     2641 gatattgctc agatctgtta gcaactgcct tgtttgttga ttctattgcc gtggattagg
     2701 gttttttttc acgagattgc ttcagatccg tacttaagat tacgtaatgg attttgattc
     2761 tgatttatct gtgattgttg actcgacagc ggccgcaccg gcgcgccatg gcagatccaa
     2821 tgaagctact gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt
     2881 gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact
     2941 ctcccaaaac caaaagatct ccgctgacta gggcacatct gacagaagtg gaatcaaggc
     3001 tagaaagact ggaacagcta tttctactga tttttccaag gtcgagcgcc cccccgaccg
     3061 atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg gcgcatgccg
     3121 acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat
     3181 ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc
     3241 agatgtttac cgatgccctt ggaattgacg agtacggtgg ggatccaatt cagcaagcca
     3301 ctgcaggagt ctcacaagac acttcggaaa atcctaacaa aacaatagtt cctgctgcat
     3361 taccacagct cacccctacc ttggtgtcac tgctggaggt gattgaaccc gaggtgttgt
     3421 atgcaggata tgatagctct gttccagatt cagcatggag aattatgacc acactcaaca
     3481 tgttaggtgg gcgtcaagtg attgcagcag tgaaatgggc aaaggcgata ccaggcttca
     3541 gaaacttaca cctggatgac caaatgaccc tgctacagta ctcatggatg tttctcatgg
     3601 catttgccct gggttggaga tcatacagac aatcaagtgg aaacctgctc tgctttgctc
     3661 ctgatctgat tattaatgag cagagaatgt ctctaccctg catgtatgac caatgtaaac
     3721 acatgctgtt tgtctcctct gaattacaaa gattgcaggt atcctatgaa gagtatctct
     3781 gtatgaaaac cttactgctt ctctcctcag ttcctaagga aggtctgaag agccaagagt
     3841 tatttgatga gattcgaatg acttatatca aagagctagg aaaagccatc gtcaaaaggg
     3901 aagggaactc cagtcagaac tggcaacggt tttaccaact gacaaagctt ctggactcca
     3961 tgcatgaggt ggttgagaat ctccttacct actgcttcca gacatttttg gataagacca
     4021 tgagtattga attcccagag atgttagctg aaatcatcac taatcagata ccaaaatatt
     4081 caaatggaaa tatcaaaaag cttctgtttc atcaaaaatg actcgaccta actgagtaag
     4141 ctagcttgtt cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc
     4201 ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat
     4261 ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt
     4321 ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt
     4381 ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa
     4441 cactagatcc ccaaacaagc ttgaaactga aggcgggaaa cgacaatctg atcatgagcg
     4501 gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt
     4561 ttggaactga catatgaaaa tattttttaa tgcattttat gacttgccaa ttgattgaca
     4621 acatgcatca atccgcggtt atgactctct taagagagtc atgaattcga gcgggaattg
     4681 atcccccctc gacagcttgc atgccggtcg actctagagg atccgggtga cagccctccg
     4741 acgggtgaca gccctccgac gggtgacagc cctccgaatt ctagaggatc cgggtgacag
     4801 ccctccgacg ggtgacagcc ctccgacggg tgacagccct ccgaattcga gctcagtacc
     4861 cggggatctg tcgacctcga tcgagatctt cgcaagaccc ttcctctata taaggaagtt
     4921 catttcattt ggagaggaca cgctgaagct agtcgactct agcctcgaat ccttaggtac
     4981 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5041 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5101 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5161 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     5221 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5281 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5341 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5401 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     5461 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     5521 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5581 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5641 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagga
     5701 tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     5761 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     5821 gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5881 aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg
     5941 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     6001 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     6061 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     6121 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
     6181 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     6241 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     6301 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     6361 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     6421 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     6481 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
     6541 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg
     6601 cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
     6661 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
     6721 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     6781 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     6841 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     6901 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
     6961 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     7021 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
     7081 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     7141 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     7201 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
     7261 tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat
     7321 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     7381 tctcgttcag ctttcttgta caaagtggtc ctaggttcgg ttcgagtatt atggcattgg
     7441 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt
     7501 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc
     7561 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt
     7621 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcaaggtt
     7681 ccagaaggta attatccaag atgtagcatc aagaatccaa tgtttacggg aaaaactatg
     7741 gaagtattat gtgagctcag caagaagcag atcaatatgc ggcacatatg caacctatgt
     7801 tcaaaaatga agaatgtaca gatacaagat cctatactgc cagaatacga agaagaatac
     7861 gtagaaattg aaaaagaaga accaggcgaa gaaaagaatc ttgaagacgt aagcactgac
     7921 gacaacaatg aaaagaagaa gataaggtcg gtgattgtga aagagacata gaggacacat
     7981 gtaaggtgga aaatgtaagg gcggaaagta accttatcac aaaggaatct tatcccccac
     8041 tacttatcct tttatatttt tccgtgtcat ttttgccctt gagttttcct atataaggaa
     8101 ccaagttcgg catttgtgaa aacaagaaaa aatttggtgt aagctatttt ctttgaagta
     8161 ctgaggatac aacttcagag aaatttgtaa gtttgtgatc cgcaaacaaa aatggtgagc
     8221 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     8281 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     8341 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     8401 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     8461 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     8521 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     8581 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     8641 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     8701 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     8761 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     8821 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     8881 ggccgccact ccaccggcgg catggacgag ctgtacaagt gagatgtgca gatcgttcaa
     8941 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     9001 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     9061 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     9121 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     9181 atccgatgat aagctgtcaa acatgaggga cacgaagtgg tttaaactat cagtgtttga
     9241 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     9301 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgccaacc acaggaaccg
     9361 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9421 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9481 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9541 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9601 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9661 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9721 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9781 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9841 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9901 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9961 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10021 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    10081 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10141 cagctaaaac aattcatcca gtaaaatata atcgaatatt atgcgcaccc gtggccagga
    10201 cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat
    10261 gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat
    10321 tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc
    10381 cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca
    10441 atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc
    10501 ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga
    10561 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
    10621 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
    10681 acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt
    10741 ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca
    10801 agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag
    10861 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
    10921 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
    10981 atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
    11041 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
    11101 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
    11161 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
    11221 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatccacag gacgggtgtg
    11281 gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg
    11341 cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca
    11401 tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc
    11461 aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg
    11521 ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa
    11581 tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
    11641 ttcttgaaaa tattgcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag
    11701 cccctggggg gatgggagct cggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
    11761 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
    11821 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
    11881 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
    11941 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
    12001 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
    12061 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
    12121 aaagaagctt tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
    12181 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
    12241 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
    12301 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
    12361 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
    12421 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
    12481 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
    12541 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
    12601 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
    12661 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
    12721 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
    12781 cttcccttcc cggtatcggt tcatgga
//
