LOCUS       sequence-462182-       10659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter 
            driving in frame epitope fusion of eGFP paired with ccdb selection 
            toxin and mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10659)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 10659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Ubi:ccdb-eGFP + mcherry
FEATURES             Location/Qualifiers
     source          1..10659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     510..529
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(929..934,935..951,952..957))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1250..1444
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1510..2583)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3012..3641)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3905..3929
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    4963..5087
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5112..5117,5118..5135,5136..5142)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5196..5855
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5262..5281)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6197..6502
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6381..6400
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6543..6667)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(6675..6677,6678..6680,6681..7394)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6675..6677,6678..6680,6681..7391)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6720..6741)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6981..7000)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7328..7349
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8197..8907
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     8610..8629
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      8916..9168
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9223..9247
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9370..9958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9450..9469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10350..10659,1..881))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
       61 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
      121 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
      181 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
      241 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
      301 aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
      361 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
      421 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
      481 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
      541 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg
      601 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
      661 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
      721 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
      781 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      841 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
      901 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
      961 tgagaattct tgaaaatatt gcgctgccat ttttggggtg aggccgttcg cggccgaggg
     1021 gcgcagcccc tggggggatg ggagctcggt gccttgatgt gggcgccggc ggtcgagtgg
     1081 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     1141 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     1201 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     1261 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     1321 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     1381 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     1441 cacaggaaag aagctttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     1501 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     1561 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     1621 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
     1681 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
     1741 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
     1801 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
     1861 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
     1921 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
     1981 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
     2041 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
     2101 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
     2161 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
     2221 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
     2281 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
     2341 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg acgcaccggc
     2401 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
     2461 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
     2521 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
     2581 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
     2641 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
     2701 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
     2761 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
     2821 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
     2881 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
     2941 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
     3001 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
     3061 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
     3121 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
     3181 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
     3241 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
     3301 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
     3361 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
     3421 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
     3481 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
     3541 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
     3601 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
     3661 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
     3721 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
     3781 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggctgatg
     3841 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     3901 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     3961 tgcggacgtt tttaatgtac tggggtggat gcagtgggcc ccactctgtg gaagacaatg
     4021 ccccgcggtc cggagtaaca gataaagcga agcttgaagg tgcatgtgac tccgtcaaga
     4081 ttacgaaacc gccaactacc acgcaaattg caattctcaa tttcctagaa ggactctccg
     4141 aaaatgcatc caataccaaa tattacccgt gtcataggca ccaagtgaca ccatacatga
     4201 acacgcgtca caatatgact ggagaagggt tccacacctt atgctataaa acgccccaca
     4261 cccctcctcc ttccttcgca gttcaattcc aatatattcc attctctctg tgtatttccc
     4321 tacctctccc ttcaaggtta gtcgatttct tctgtttttc ttcttcgttc tttccatgaa
     4381 ttgtgtatgt tctttgatca atacgatgtt gatttgattg tgttttgttt ggtttcatcg
     4441 atcttcaatt ttcataatca gattcagctt ttattatctt tacaacaacg tccttaattt
     4501 gatgattctt taatcgtaga tttgctctaa ttagagcttt ttcatgtcag atccctttac
     4561 aacaagcctt aattgttgat tcattaatcg tagattaggg cttttttcat tgattacttc
     4621 agatccgtta aacgtaacca tagatcaggg ctttttcatg aattacttca gatccgttaa
     4681 acaacagcct tattttttat acttctgtgg tttttcaaga aattgttcag atccgttgac
     4741 aaaaagcctt attcgttgat tctatatcgt ttttcgagag atattgctca gatctgttag
     4801 caactgcctt gtttgttgat tctattgccg tggattaggg ttttttttca cgagattgct
     4861 tcagatccgt acttaagatt acgtaatgga ttttgattct gatttatctg tgattgttga
     4921 ctcgacagcg gccgcacccc cgggggcgcg cccttaggta ccacaagttt gtacaaaaaa
     4981 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     5041 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     5101 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     5161 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     5221 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     5281 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     5341 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     5401 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     5461 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     5521 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     5581 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     5641 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     5701 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     5761 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     5821 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     5881 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     5941 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     6001 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     6061 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     6121 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     6181 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     6241 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     6301 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     6361 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     6421 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     6481 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     6541 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     6601 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     6661 aagtggtgat atcaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6721 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     6781 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     6841 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     6901 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6961 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     7021 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     7081 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     7141 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     7201 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     7261 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     7321 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     7381 agctgtacaa gtaacctagg ttcggttcga gtattatggc attgggaaaa ctgtttttct
     7441 tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact
     7501 gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca
     7561 aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga
     7621 tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatca aggttccaga aggtaattat
     7681 ccaagatgta gcatcaagaa tccaatgttt acgggaaaaa ctatggaagt attatgtgag
     7741 ctcagcaaga agcagatcaa tatgcggcac atatgcaacc tatgttcaaa aatgaagaat
     7801 gtacagatac aagatcctat actgccagaa tacgaagaag aatacgtaga aattgaaaaa
     7861 gaagaaccag gcgaagaaaa gaatcttgaa gacgtaagca ctgacgacaa caatgaaaag
     7921 aagaagataa ggtcggtgat tgtgaaagag acatagagga cacatgtaag gtggaaaatg
     7981 taagggcgga aagtaacctt atcacaaagg aatcttatcc cccactactt atccttttat
     8041 atttttccgt gtcatttttg cccttgagtt ttcctatata aggaaccaag ttcggcattt
     8101 gtgaaaacaa gaaaaaattt ggtgtaagct attttctttg aagtactgag gatacaactt
     8161 cagagaaatt tgtaagtttg tgatccgcaa acaaaaatgg tgagcaaggg cgaggaggat
     8221 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     8281 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     8341 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     8401 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     8461 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     8521 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     8581 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     8641 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     8701 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     8761 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     8821 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     8881 ggcggcatgg acgagctgta caagtgagat gtgcagatcg ttcaaacatt tggcaataaa
     8941 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     9001 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     9061 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     9121 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatccg atgataagct
     9181 gtcaaacatg agggacacga agtggtttaa actatcagtg tttgacagga tatattggcg
     9241 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     9301 aggtttatcc gttcgtccat ttgtatgtgc caaccacagg aaccgtaaaa aggccgcgtt
     9361 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9421 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9481 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9541 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9601 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9661 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9721 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9781 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9841 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9901 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9961 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10021 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10081 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct aaaacaattc
    10141 atccagtaaa atataatcga atattatgcg cacccgtggc caggacccaa cgctgcccga
    10201 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
    10261 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
    10321 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
    10381 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
    10441 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa
    10501 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
    10561 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
    10621 ataatgggga aggccatcca gcctcgcgtc gcgaacgcc
//
