LOCUS       sequence-46220-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA,
            Settleman J
  TITLE     TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers.
  JOURNAL   Cell. 2012 Feb 17;148(4):639-50.
  PUBMED    22341439
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-02-17"; volume: "148(4)"; pages: "639-50"
COMMENT     Sequence Label: pLenti-PGK-KRAS4B(G12V)
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(81..611,612..614,615..644))
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="TEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             complement(648..674)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(683..707)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(825..843)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgag cggccgccac tgtgctggat atcaaccact
       61 ttgtacaaga aagctgggtc ttacataatt acacactttg tctttgactt ctttttcttc
      121 tttttaccgt ctttgctcat cttttcttta tgttttcgaa tttctcgaac taatgtatag
      181 aaggcatcat caacaccctg tcttgtcttt gctgatgttt caataaaagg aattccataa
      241 cttcttgcta agtcctgagc ctgttttgtg tctactgttc tagaaggcaa atcacattta
      301 tttcctacta ggaccatagg tacatcttca gagtccttaa ctcttttaat ttgttctcta
      361 taatggtgaa tatcttcaaa tgatttagta ttatttatgg caaatacaca aagaaagccc
      421 tccccagtcc tcatgtactg gtccctcatt gcactgtact cctcttgacc tgctgtgtcg
      481 agaatatcca agagacaggt ttctccatca attactactt gcttcctgta ggaatcctct
      541 attgttggat catattcgtc cacaaaatga ttctgaatta gctgtatcgt caaggcactc
      601 ttgcctacgc caacagctcc aactaccaca agtttatatt cagtatcagc gtaatctgga
      661 acatcgtatg ggtacatggt gaagcctgct tttttgtaca aacttgttga tatctgcaga
      721 attccaccac actggactag tcgacgaacc ggtggatccc cctggggaga gaggtcggtg
      781 attcggtcaa cgagggagcc gactgccgac gtgcgctccg gaggcttgca gaatgcggaa
      841 caccgcgcgg gcaggaacag ggcccacact accgccccac accccgcctc ccgcaccgcc
      901 ccttcccgg
//
