LOCUS       sequence-46221-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA,
            Settleman J
  TITLE     TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers.
  JOURNAL   Cell. 2012 Feb 17;148(4):639-50.
  PUBMED    22341439
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-02-17"; volume: "148(4)"; pages: "639-50"
COMMENT     Sequence Label: pLenti-PGK-KRAS4A(G12V)
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(656..697)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(718..742)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(860..878)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 aattttgtat ccagaggttg attgtcgagc ggccgccact gtgctggata tcaaccactt
       61 tgtacaagaa agctgggtct tacattataa tgcatttttt aattttcaca cagccaggag
      121 tcttttcttc tttgctgatt tttttcaatc tgtattgtcg gatctctctc accaatgtat
      181 aaaaagcatc ctccactctc tgtcttgtct ttgctgatgt ttcaataaaa ggaattccat
      241 aacttcttgc taagtcctga gcctgttttg tgtctactgt tctagaaggc aaatcacatt
      301 tatttcctac taggaccata ggtacatctt cagagtcctt aactctttta atttgttctc
      361 tataatggtg aatatcttca aatgatttag tattatttat ggcaaataca caaagaaagc
      421 cctccccagt cctcatgtac tggtccctca ttgcactgta ctcctcttga cctgctgtgt
      481 cgagaatatc caagagacag gtttctccat caattactac ttgcttcctg taggaatcct
      541 ctattgttgg atcatattcg tccacaaaat gattctgaat tagctgtatc gtcaaggcac
      601 tcttgcctac gccaacagct ccaactacca caagtttata ttcagtcccg ggacgcgtag
      661 aatcgagacc gaggagaggg ttagggatag gcttacccat ggttctatct ccttcgaagc
      721 ctgctttttt gtacaaactt gttgatatct gcagaattcc accacactgg actagtcgac
      781 gaaccggtgg atccccctgg ggagagaggt cggtgattcg gtcaacgagg gagccgactg
      841 ccgacgtgcg ctccggaggc ttgcagaatg cggaacaccg cgcgggcagg aacagggccc
      901 acactaccgc cc
//
