LOCUS       sequence-46222-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA,
            Settleman J
  TITLE     TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers.
  JOURNAL   Cell. 2012 Feb 17;148(4):639-50.
  PUBMED    22341439
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-02-17"; volume: "148(4)"; pages: "639-50"
COMMENT     Sequence Label: pLenti-PGK-ER-KRAS(G12V)
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(80..610,611..613,614..643))
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="TEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
ORIGIN
        1 aattttgtat ccagaggttg attgtcgagc ggccgccact gtgctggata tcaaccactt
       61 tgtacaagaa agctgggtct tacataatta cacactttgt ctttgacttc tttttcttct
      121 ttttaccgtc tttgctcatc ttttctttat gttttcgaat ttctcgaact aatgtataga
      181 aggcatcatc aacaccctgt cttgtctttg ctgatgtttc aataaaagga attccataac
      241 ttcttgctaa gtcctgagcc tgttttgtgt ctactgttct agaaggcaaa tcacatttat
      301 ttcctactag gaccataggt acatcttcag agtccttaac tcttttaatt tgttctctat
      361 aatggtgaat atcttcaaat gatttagtat tatttatggc aaatacacaa agaaagccct
      421 ccccagtcct catgtactgg tccctcattg cactgtactc ctcttgacct gctgtgtcga
      481 gaatatccaa gagacaggtt tctccatcaa ttactacttg cttcctgtag gaatcctcta
      541 ttgttggatc atattcgtcc acaaaatgat tctgaattag ctgtatcgtc aaggcactct
      601 tgcctacgcc aacagctcca actaccacaa gtttatattc agtttcgtcg actgcagaat
      661 tcgaagcttg gatcgtgttg gggaagccct ctgcttccgg gggtatgtag taggtttgta
      721 aggaatgtgc tgaagtggag ctggtggtgg ccagctgggt ctggctgggc tcctctgggg
      781 gcactcccat gcgactggct ggggcatgaa ggcggtgggc atccaacatc tccaggagca
      841 ggtcatagag ggg
//
