LOCUS       sequence-462228-        6906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""Expresses the rabies glycoprotein"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Wertz A, Trenholm S, Yonehara K, Hillier D, Raics Z, Leinweber M, 
            Szalay G, Ghanem A, Keller G, Rozsa B, Conzelmann KK, Roska B
  TITLE     PRESYNAPTIC NETWORKS. Single-cell-initiated monosynaptic tracing 
            reveals layer-specific cortical network modules.
  JOURNAL   Science. 2015 Jul 3;349(6243):70-4. doi: 10.1126/science.aab1687.
  PUBMED    26138975
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-07-3- 3"; volume: "349"; issue: 
            "6243"; pages: "70-4"
COMMENT     Sequence Label: pAAV-EF1a-CVS-G-WPRE-pGHpA
FEATURES             Location/Qualifiers
     source          1..6906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   651..780
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        881..2059
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1112..2050
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2007..2027
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2078..2087
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3683..4271
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3736..3756)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4154..4165)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4303..4779
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4502..4521)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4819..4959
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4819..4948
                     /label=AAV2 ITR
     rep_origin      5034..5489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5121..5140)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5331..5352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5506..5525)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5625..5647
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5685..5703)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5771..5875
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5876..5944,5945..6736)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6094..6113)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
      841 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
      901 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      961 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     1021 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     1081 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     1141 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     1201 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     1261 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     1321 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     1381 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     1441 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     1501 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     1561 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1621 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1681 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1741 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1801 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1861 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1921 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1981 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     2041 tccatttcag gtgtcgtgag gtaccggatc ctctagagcc accatggttc ctcaggttct
     2101 tttgtttgta ctccttctgg ggttttcgtt gtgtttcggg aagttcccca tttacacgat
     2161 accagacaaa cttggtccct ggagccctat tgacatacac catctccgct gtccaaataa
     2221 cctggttgtg gaggatgaag gatgtaccaa cctgtccggg ttctcctata tggaactcaa
     2281 agtgggatac atctcagcca tcaaagtgaa cgggttcact tgcacaggtg ttgtgacaga
     2341 ggcagagacc tacaccaact ttgttggtta tgtcacaacc acattcaaga gaaagcattt
     2401 ccgccccacc ccagacgcat gtagagccgc gtataactgg aagatggccg gtgaccccag
     2461 atatgaagag tccctacaaa atccataccc cgactaccac tggcttcgaa ctgtaagaac
     2521 caccaaagag tccctcatta tcatatcccc aagtgtgaca gatttggacc catatgacaa
     2581 atcccttcac tcaagggtct tccctggcgg aaagtgctca ggaataacgg tgtcctctac
     2641 ctactgctca actaaccatg attacaccat ttggatgccc gaggacccga gaccagggac
     2701 accttgtgac atttttacca atagcagagg gaagagagca tccaacggga acaagacttg
     2761 cggctttgtg gatgaaagag gcctgtataa gtctctaaaa ggagcatgca ggctcaagtt
     2821 atgtggagtt cttggactta gacttatgga tggaacatgg gtcgcgatgc aaacatcaga
     2881 tgagaccaaa tggtgctctc cagatcagtt ggtgaatttg cacgactttc gctcaaacga
     2941 gattgagcat ctcgttgtgg aggatttagt caagaaaaga gaggaatgtc tggatacatt
     3001 agagtccatc atgaccacca agtcagtaag tttcagacgt ctcagtcacc tgagaaaact
     3061 tgtcccaggg tttggaaaag catataccat attcaacaaa accttgatgg aggctgatgt
     3121 tcactacaag tcagtccgga cctggaatga gatcatcccc tcaaaagggt gtttgaaagt
     3181 tggaggaagg tgccatcctc atgtgaacgg ggtgtttttc aatggtataa tattagggcc
     3241 tgacgaccgt gtcctaatcc cagagatgca atcatccctc ctccggcaac atatggagtt
     3301 gttggaatct tcagttatcc ccctgatgca ccccctggct gacccttcta cagttttcaa
     3361 agaaggtgat gaggctgagg attttgttga agttcacctc cccgatgtgt acaaaaagat
     3421 ctcaggggtt gacctgggtc tcccgaactg gggaaagtat gtattgatga ctgcaggggc
     3481 catgattggc ctggtgttga tattttccct aatgacatgg tgcagaagag ccaatcgacc
     3541 agaatcgaaa caacgcagtt ttggagggac aggggggaat gtgtcagtca cttcccaaag
     3601 cggaaaagtc ataccttcat gggaatcata taagagtgga ggtgagatca gactgtaaga
     3661 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     3721 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3781 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3841 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3901 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3961 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4021 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     4081 atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt
     4141 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4201 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4261 cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac
     4321 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     4381 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     4441 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     4501 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     4561 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     4621 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     4681 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     4741 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg
     4801 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4861 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4921 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     4981 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     5041 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5101 tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5161 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5221 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     5281 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5341 gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat
     5401 tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5461 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     5521 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5581 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5641 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     5701 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5761 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5821 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5881 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5941 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6001 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6061 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6121 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6181 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6241 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6301 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6361 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6421 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6481 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6541 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6601 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6661 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6721 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6781 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6841 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6901 atcttc
//
