LOCUS       sequence-462235-       14793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac cargo vector encoding PEmax and puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14793)
  AUTHORS   Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo 
            MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC
  TITLE     Open-ended molecular recording of sequential cellular events into 
            DNA
  JOURNAL   bioRxiv 2021.11.05.467507
REFERENCE   2  (bases 1 to 14793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.05.467507"
COMMENT     Sequence Label: pTBL3342 PB-PEmax-puro
FEATURES             Location/Qualifiers
     source          1..14793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        922..1225
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1508..2524
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2448..2470
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2532..2551
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2621..2641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2642..6742
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6800..6820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8921..8941
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8951..8977
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     primer_bind     complement(8998..9015)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9004..9228
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9504..10715
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     10552..10571
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             10722..11324
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10722..10741)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11218..11238
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(11412..11431)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11477..11532
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11531..11550)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   12471..12533
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(12584..12602)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(12642..13457)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(12642..13457)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     13365..13384
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     13449..13468
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      13682..14270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14171..14190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14382..14401
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14382..14400
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   14430..14464
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ctgaactggc cgataattgc agacgatcga attcgagttg gcgcgcctgt cattctaaat
       61 ctctctttca gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag
      121 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc
      181 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg
      241 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggagg
      301 gggctgtccc cgtgagctcc caggcgcgcc tgtcattcta aatctctctt tcagcctaaa
      361 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
      421 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
      481 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
      541 agcgggggag ggacgtaatt acatccctgg gggctttggg agggggctgt ccccgtgagc
      601 tcccaggcgc gcctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc
      661 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
      721 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
      781 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
      841 attacatccc tgggggcttt gggggggggc tgtccccgtg agctcaataa aagagcccac
      901 aacgccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      961 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1021 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1081 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1141 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1201 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     1261 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     1321 agcgatgggg gcgggggggg gggggggggg gcgcgcgcsg gggggggggg gggggggsgg
     1381 gggggggggg ggggsgrggs ggrgrggtgc ggcggcagcc aatcagagcg gcgcgctccg
     1441 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     1501 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     1561 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     1621 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     1681 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     1741 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     1801 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     1861 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     1921 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     1981 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     2041 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggkgg gggtgccggg
     2101 cggggcgggg ccgcckcggg ccggggrggg ctcgggggrg gggcgcggcg gcccccggag
     2161 tgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     2221 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     2281 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     2341 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     2401 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     2461 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     2521 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcgc
     2581 cactatgaaa cggacagccg acggaagcga gttcgagtca ccaaagaaga agcggaaagt
     2641 cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
     2701 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
     2761 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
     2821 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
     2881 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     2941 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     3001 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     3061 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     3121 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     3181 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     3241 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagaaa
     3301 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
     3361 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     3421 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     3481 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     3541 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     3601 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     3661 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     3721 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     3781 aaagatggac ggcaccgagg aactgctcgt gaagctgaag agagaggacc tgctgcggaa
     3841 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     3901 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     3961 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     4021 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     4081 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     4141 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     4201 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     4261 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     4321 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     4381 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     4441 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     4501 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     4561 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     4621 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     4681 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     4741 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     4801 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     4861 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     4921 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     4981 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     5041 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     5101 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggacgctat
     5161 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     5221 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     5281 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     5341 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     5401 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     5461 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
     5521 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     5581 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     5641 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     5701 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     5761 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     5821 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     5881 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     5941 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     6001 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
     6061 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     6121 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     6181 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     6241 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
     6301 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     6361 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     6421 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     6481 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     6541 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     6601 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
     6661 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     6721 cctgtctcag ctgggaggtg actccggcgg aagctctggt ggcagcaagc ggaccgccga
     6781 cggctctgaa ttcgagagcc ctaagaagaa aagaaaggtg agcggaggct ctagcggcgg
     6841 aagcaccctg aacattgaag acgagtatag actgcatgaa acaagcaagg aacccgacgt
     6901 gtccctgggc tccacctggc tgtccgactt tccccaggcc tgggccgaga caggaggaat
     6961 gggcctggcc gtgcggcagg cacccctgat catccctctg aaggccacct ctacacccgt
     7021 gagcatcaag cagtacccta tgtctcagga ggccagactg ggcatcaagc ctcacatcca
     7081 gaggctgctg gaccagggca tcctggtgcc atgccagagc ccctggaaca caccactgct
     7141 gcccgtgaag aagccaggca ccaatgacta tagacccgtg caggatctga gagaggtgaa
     7201 caagagggtg gaggatatcc accccaccgt gcccaaccct tacaatctgc tgtccggcct
     7261 gcccccttct caccagtggt atacagtgct ggacctgaag gatgccttct tttgtctgag
     7321 actgcaccct accagccagc cactgttcgc ctttgagtgg agggaccctg agatgggcat
     7381 ctctggccag ctgacctgga cacgcctgcc tcagggcttc aagaatagcc caacactgtt
     7441 taacgaggcc ctgcaccgcg acctggcaga tttccggatc cagcacccag atctgatcct
     7501 gctgcagtac gtggacgatc tgctgctggc cgccaccagc gagctggatt gccagcaggg
     7561 aacacgcgcc ctgctgcaga ccctgggaaa cctgggatat agggcatccg ccaagaaggc
     7621 ccagatctgt cagaagcagg tgaagtacct gggctatctg ctgaaggagg gccagagatg
     7681 gctgacagag gccaggaagg agacagtgat gggccagcca acacccaaga ccccaagaca
     7741 gctgagggag ttcctgggca aagcaggatt ttgcaggctg ttcatcccag gattcgcaga
     7801 gatggcagca cctctgtacc cactgaccaa gccgggcacc ctgtttaatt ggggccctga
     7861 ccagcagaag gcctatcagg agatcaagca ggccctgctg acagcaccag ccctgggcct
     7921 gccagacctg accaagcctt tcgagctgtt tgtggatgag aagcagggct acgccaaggg
     7981 cgtgctgacc cagaagctgg gaccatggag gcggcccgtg gcctatctgt ccaagaagct
     8041 ggacccagtg gcagcaggat ggccaccatg cctgaggatg gtggcagcaa tcgccgtgct
     8101 gacaaaggat gccggcaagc tgaccatggg acagccactg gtcatcctgg caccacacgc
     8161 agtggaggcc ctggtgaagc agcctccaga tcgctggctg tctaacgccc ggatgacaca
     8221 ctaccaggcc ctgctgctgg acaccgatcg cgtgcagttt ggccctgtgg tggccctgaa
     8281 tccagccacc ctgctgcctc tgccagagga gggcctgcag cacaactgtc tggacatcct
     8341 ggcagaggca cacggaacaa ggccagacct gaccgatcag cccctgcctg acgccgatca
     8401 cacatggtat accgatggaa gctccctgct gcaggagggc cagaggaagg caggagcagc
     8461 agtgaccaca gagacagaag tgatctgggc caaggccctg ccagcaggca catccgccca
     8521 gcgggccgag ctgatcgccc tgacccaggc cctgaagatg gccgagggca agaagctgaa
     8581 cgtgtacaca gactccagat atgccttcgc caccgcacac atccacggag agatctacag
     8641 gcgccggggc tggctgacct ctgagggcaa ggagatcaag aacaaggatg agatcctggc
     8701 cctgctgaag gccctgtttc tgcccaagcg gctgagcatc atccactgtc ctggacacca
     8761 gaagggacac tccgccgagg caaggggcaa tcggatggcc gaccaggccg ccagaaaggc
     8821 tgctattact gaaactcccg acacttccac tctgctgatt gaaaactcct ccccttctgg
     8881 cggctcaaaa agaaccgccg acggcagcga attcgagtct cccaagaaga agaggaaagt
     8941 cggctctggc cctgccgcta agagagtgaa gctggacgga tcctaactcg agcgacgcct
     9001 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     9061 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9121 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9181 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggc tgaagtgaat
     9241 aaagctccac acagtcgatg tttctatatg ttttaagtct taggcatatc ggacatcact
     9301 agtgcatcag cgccctctgc tggttcagta agaatggttt ccccatatac tgggcaaaac
     9361 tggatttttg tggtgatgaa agggaaaaaa acaaattcag tacaattgga aagctggtgt
     9421 tgttttaaaa ctcttgaaaa acactgaatg aaaggaatca cactaaaact atatgttgca
     9481 atgttgttgg ttaaaacgga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     9541 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     9601 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     9661 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     9721 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     9781 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     9841 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     9901 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     9961 acggaagcgt gtggagacac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
    10021 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
    10081 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
    10141 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
    10201 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
    10261 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
    10321 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
    10381 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
    10441 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
    10501 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
    10561 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
    10621 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
    10681 ctaaattctg gccgtttttg gcttttttgt tagacgccac tatgaccgag tacaagccca
    10741 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
    10801 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
    10861 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
    10921 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
    10981 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
    11041 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    11101 ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    11161 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggaaacctcc gcgccccgca
    11221 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    11281 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcatc ctaactcgag attcactcct
    11341 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa
    11401 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
    11461 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
    11521 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
    11581 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
    11641 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
    11701 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
    11761 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
    11821 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
    11881 ggcgtaatgc tggaaatctg ctcgtcagtg gtgctcacac tgacgaatca tgtacagatc
    11941 ataccgatga ctgcctggcg actcacaact aagcaagaca gccggaacca gcgccggcga
    12001 acaccactgc atatatggca tatcacaaca gtccaagcag ttacagagat gttacgaacc
    12061 ctgaacaggt tgactattaa ttgtgtctgc ttgatgtgga caccaggtgg cgctggacat
    12121 cagatttgga gaggcagttg tctagggaac cgggctctgt gccagcgcag gaggcaggct
    12181 ggctctccta ttccagggat gctcatccag gaaggaaagg ttgcatgctg gacacactaa
    12241 ccttacagcg gtgggctcta tggggtaccc acgcatggta tggatgaaca ctagttaaaa
    12301 gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
    12361 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
    12421 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
    12481 ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaatct
    12541 agtatacgcg tatgcggccg cttaattaat ccagcttttg ttccctttag tgagggttaa
    12601 ttccgagcgg ccgcgtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc
    12661 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
    12721 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
    12781 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
    12841 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
    12901 aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
    12961 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gaggcgaaat
    13021 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
    13081 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
    13141 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
    13201 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
    13261 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
    13321 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
    13381 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctggagca agacgtttcc
    13441 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
    13501 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
    13561 tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagtcatg accaaaatcc
    13621 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    13681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    13741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    13801 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    13861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    13921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    13981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    14041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    14101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    14161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    14221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    14281 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    14341 cgttatcccc tgattctgtg gataaccgtg cggccgccaa ttaatacgac tcactatagg
    14401 gcgaattggg gcgcgccatt ctagattaac cctagaaaga tagtctgcgt aaaattgacg
    14461 catgcattct tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt
    14521 taggacatct cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg
    14581 tcactgattt tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg
    14641 tgacttttaa gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg
    14701 ataacttatt atatatatat tttcttgtta tagatatcaa ctagaatgct agcatgggcc
    14761 cagatctcca tgggccctga attcgcatct aga
//
