LOCUS       sequence-46229-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Liu M, Horowitz A
  TITLE     A PDZ-binding motif as a critical determinant of Rho guanine 
            exchange factor function and cell phenotype.
  JOURNAL   Mol Biol Cell. 2006 Apr;17(4):1880-7. Epub 2006 Feb 8.
  PUBMED    16467373
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2006-04"; volume: "17(4)"; pages: "1880-7. Epub 2006 
            Feb 8"
COMMENT     Sequence Label: pEGFP-Syx2
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..230
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      272..281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(320..523)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(349..369)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        524..827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
       61 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      121 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      181 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      241 accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtag cgctagcgga
      301 tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc
      361 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
      421 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
      481 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
      541 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
      601 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
      661 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
      721 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
      781 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
      841 atgggctatg aactaatgac cccgtaattg attactatta ataactaa
//
