LOCUS       sequence-462293-        8726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry-tagged SopF in mammalian cells. Stable expression 
            with piggybac transposase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Bentley-DeSousa A, Roczniak-Ferguson A, Ferguson SM
  TITLE     A STING-CASM-GABARAP pathway activates LRRK2 at lysosomes.
  JOURNAL   J Cell Biol. 2025 Feb 3;224(2):e202310150. doi: 
            10.1083/jcb.202310150. Epub 2025 Jan 15.
  PUBMED    39812709
REFERENCE   2  (bases 1 to 8726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-02-3"; volume: "224(2):e202310150. doi"; pages: 
            " 10.1083/jcb.202310150. Epub 2025 Jan 15"
COMMENT     Sequence Label: pPB-mCherry-SopF
FEATURES             Location/Qualifiers
     source          1..8726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..33
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(252..356)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(382..837)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(519..540)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     731..750
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     964..986
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     978..995
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     979..995
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1002..1021
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1002..1020
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1087..1149
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1499..1723
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1712..1729
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1767..2162)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(2198..2401)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2227..2247)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2402..2781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3118..3137
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3136..3191
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3237..3256
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     protein_bind    3334..3358
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4520..5227)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4795..4814)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5064..5082
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      5224..5233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5234..5258)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5258..6436)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5267..6205
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5290..5310)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(6437..6457)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     repeat_region   6734..6768
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6917..6935)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6956..6972)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6956..6972)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6969..6991)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6980..6996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7004..7010,7011..7028,7029..7034))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7049..7070
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7187..7204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7358..7946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7438..7457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8117..8726,1..182,183..251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
       61 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      361 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
      421 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
      481 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
      541 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
      601 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
      661 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
      721 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
      781 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
      841 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      901 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      961 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
     1021 gcgaattgga gctcggtatt cacgacagca ggctgaataa taaaaaaatt agaaactatt
     1081 atttaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgcgtaaaat
     1141 tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt
     1201 aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg
     1261 tttatttatt tattaaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact
     1321 tttaaacatt ctctctttta caaaaataaa cttattttgt actttaaaaa cagtcatgtt
     1381 gtattataaa ataagtaatt agcttaacct atacataata gaaacaaatt atacttatta
     1441 gtcagtcaga aacaactttg gcacatatca atattatgct ctcgttaatt aactcgagcc
     1501 atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg
     1561 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt
     1621 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg
     1681 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg
     1741 gtttaaacgg gccctctaga ctcgagttag ccctcccaca cataaccaga gggcagcaat
     1801 tcacgaatcc caactgccgt cggctgtcca tcactgtcct tcactatggc tttgatccca
     1861 ggatgcagat cgagaagcac ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc
     1921 tcatttccga tcgcgacgat acaagtcagg ttgccagctg ccgcagcagc agcagtgccc
     1981 agcaccacga gttctgcaca aggtccccca gtaaaatgat atacattgac accagtgaag
     2041 atgcggccgt cgctagagag agctgcgctg gcgacgctgt agtcttcaga gatggggatg
     2101 ctgttgattg tagccgttgc tctttcaatg agggtggatt cttcttgaga caaaggcttg
     2161 gcaatggtgg cgcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc
     2221 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     2281 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     2341 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     2401 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     2461 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     2521 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     2581 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     2641 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     2701 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     2761 aactagtcaa taatcaatgt caacgcgaag cttgggctgc aggtcgaggg atctccataa
     2821 gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc tggctagtaa
     2881 aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatataaa
     2941 aaaaatctaa cctcaagtca aggcttttct atggaataag gaatggacag cagggggctg
     3001 tttcatatac tgatgacctc tttatagcca acctttgttc atggcagcca gcatatgggc
     3061 atatgttgcc aaactctaaa ccaaatactc attctgatgt tttaaatgat ttgccctccc
     3121 atatgtcctt ccgagtgaga gacacaaaaa attccaacac actattgcaa tgaaaataaa
     3181 tttcctttat tagccagaag tcagatgctc aaggggcttc atgatgtccc cataattttt
     3241 ggcagaggga aaaagatctc agtggtattt gtgagccagg gcattggcca caccagccac
     3301 caccttctga taggcagcct gcacctgagg atcaccactt tgtacaagaa agctgggttc
     3361 aatataatat tatgcagtct ctattaagcg cctggtttat ttgttttgca taatcatata
     3421 gttgactttt cgagtaagag ttttcttgca aagacaaata aacgtgtttt atatctctga
     3481 ataaacatac atcaccatga atatgagcct ctatataatt cccttcatag cctttaccat
     3541 aattagaatg agctaaaaat ttttcgtcct tagccatttt aaccaaactc ttaaagcaat
     3601 tataaccaaa aaaatcattt tgacaggatg caatcaggtt ctccatatgc caaaatgtag
     3661 ataatttact cgtatccagg ccaaatctgt ggccgtagat atcaaaaggt gagaatgtac
     3721 agtttgtttt tacattatca tttaattcaa aaaatgattt cccataggcg ctggcacctc
     3781 cattttcacc gttcagaaag tccagtgcag cataaattgg tctgcttgta gtgctaaaag
     3841 ttctactgtt gggagtatat gctacggaaa aaccgcctgt ctgaccatat ggggcataag
     3901 gtgaatctgc aagcttctcc agttcaaatg ctttagtttc aactgaatca cgcccgacat
     3961 tataagcagg taaatctccc ggtctgcaac ctaatgcata agagttcaga tattctttat
     4021 tttttaagag agagacaaag tcaatttttg ctgcattaaa atttattgtc agccgggcat
     4081 tttgtaaaat atccaccatc ttatttagta agagagcgca atctatttcg gcaccacact
     4141 cacggcttat ccgcctgagc gctttttctc ttattatgtc agcgtcgcgc tgacacctgg
     4201 aatgaatatg cgcaagtact tgttttccaa aaaggcgacc atacaccttt ttacgctctt
     4261 cattgctgag accgcaaaac acttcgtcaa aagaaagcct gtacgctgcg cttacagaac
     4321 ctctcgccgt tctgctttct ggaaatagcg gaacatcttc aacaacattt ctaacttgct
     4381 gaatgtctga agacagtgga gtacgtccgg catttttttc cttatctgtt tccagatatt
     4441 ccggaacctt tatacttcca ctatggcaga taggtttgag catagaattc gaagcttgag
     4501 ctcgagatct gagtccggac ttgtacagct cgtccatgcc gccggtggag tggcggccct
     4561 cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact
     4621 tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct
     4681 tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct
     4741 tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc atggtcttct
     4801 tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga
     4861 actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt
     4921 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag tcggggatgt
     4981 cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac aggatgtccc
     5041 aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
     5101 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca
     5161 tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc
     5221 tcaccatggt ggcagcctgc ttttttgtac aaacttgtca cgacacctga aatggaagaa
     5281 aaaaactttg aaccactgtc tgaggcttga gaatgaacca agatccaaac tcaaaaaggg
     5341 caaattccaa ggagaattac atcaagtgcc aagctggcct aacttcagtc tccacccact
     5401 cagtgtgggg aaactccatc gcataaaacc cctcccccca acctaaagac gacgtactcc
     5461 aaaagctcga gaactaatcg aggtgcctgg acggcgcccg gtactccgtg gagtcacatg
     5521 aagcgacggc tgaggacgga aaggcccttt tcctttgtgt gggtgactca cccgcccgct
     5581 ctcccgagcg ccgcgtcctc cattttgagc tccctgcagc agggccggga agcggccatc
     5641 tttccgctca cgcaactggt gccgaccggg ccagccttgc cgcccagggc ggggcgatac
     5701 acggcggcgc gagaccaggc accagagcag gccggccagc ttgagactac ccccgtccga
     5761 ttctcggtgg ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg
     5821 ccccgtcgcc gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct tggcccgcat
     5881 ttacaagact atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg
     5941 ctagagactt atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc
     6001 ggccccagcg cccaggccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc
     6061 aaggcctcga actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact
     6121 gcagccaggt ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc
     6181 cgcgggaacc acacacggca cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa
     6241 cgttcacggc gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga
     6301 gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc
     6361 aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact
     6421 gacgggcacc ggagcccaac ttttctatac aaagttgatg atatctataa caagaaaata
     6481 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
     6541 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
     6601 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
     6661 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
     6721 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aaaaaagatt
     6781 tgcgctttac tcgacctaaa ctttaaacac gtcatagaat cttcgtttga caaaaaccac
     6841 attgtggcca agctgtgtga cgcgacgcgc gctaaagaat ggcaaaccaa gtcgcgcgag
     6901 gtaccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
     6961 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7021 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7081 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7141 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7201 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7261 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7321 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7381 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7441 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7501 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7561 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7621 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7681 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7741 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7801 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7861 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7921 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7981 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8041 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8101 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8161 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8221 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8281 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8341 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8401 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8461 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8521 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8581 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8641 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8701 tgcggcgacc gagttgctct tgcccg
//
