LOCUS       sequence-462308-        9614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses estradiol-inducible CRISPR guide RNA and repair template 
            for creating a frameshift mutation in ADE2 gene of yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Stuecker TN, Hood SE, Molina Pineda J, Lenaduwe S, Winter J, Sadhu 
            MJ, Lewis JA
  TITLE     Improved vectors for retron-mediated CRISPR-Cas9 genome editing in 
            Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2025 Aug 4:jkaf175. doi: 10.1093/g3journal/jkaf175.
  PUBMED    40758833
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2025 Aug 4:jkaf175. doi: 10.1093/g3journal/jkaf175."
COMMENT     Sequence Label: pCRISPEY-Z3-Hyg-ADE2
FEATURES             Location/Qualifiers
     source          1..9614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(183..202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(184..202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(212..229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(212..228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(221..243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     gene            complement(350..1925)
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     terminator      complement(350..547)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(553..1581)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             complement(553..1581)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     promoter        complement(1582..1925)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     rep_origin      2038..2493
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2125..2144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2335..2356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2624..3427)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3428..3643)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(3667..3686)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3786..3808
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3846..3864)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3905..4408
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        4445..4549
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4550..4618,4619..5410)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4768..4787)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5581..6169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6070..6089
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8914..8937
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 aaaaaaaaaa aaagaaaaaa gaattttagg aatacaatgc agcttgtaag taaaatctgg
       61 aatattcata tcgccacaac ttcttatgct tataaaagca ctaatgcctg aatttatgtt
      121 gaaaatatgt gtcacaaata aagaaactgt gacatctgac acatttccac gtacccaatt
      181 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
      241 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagtc
      301 gagagctcgt tttcgacact ggatggcggc gttagtatcg aatcgacagc agtatagcga
      361 ccagcattca catacgattg acgcatgata ttactttctg cgcacttaac ttcgcatctg
      421 ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct aacatttgat taaaatagaa
      481 caactacaat ataaaaaaac tatacaaatg acaagttctt gaaaacaaga atctttttat
      541 tgtcagtact gattattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
      601 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
      661 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
      721 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
      781 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
      841 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
      901 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
      961 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
     1021 ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
     1081 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
     1141 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
     1201 atcggccgca gcaatggcat ccattgcctc cgcgaccggc tgcagaacag cgggcagttc
     1261 ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag
     1321 gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa
     1381 gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac
     1441 atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg
     1501 catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt
     1561 gagttcaggc tttttaccca tggttgttta tgttcggatg tgatgtgaga actgtatcct
     1621 agcaagattt taaaaggaag tatatgaaag aagaacctca gtggcaaatc ctaacctttt
     1681 atatttctct acaggggcgc ggcgtgggga caattcaacg cgtctgtgag gggagcgttt
     1741 ccctgctcgc aggtctgcag cgaggagccg taatttttgc ttcgcgccgt gcggccatca
     1801 aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg gctaaatgta cgggcgacag
     1861 tcacatcatg cccctgagct gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc
     1921 atgtcgctgg ccgggtgacc cggcggggac gaggcaagct aaacagactg gcgtaatagc
     1981 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     2041 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2101 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2161 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2221 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     2281 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2341 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2401 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2461 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt gaagctctaa
     2581 tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc
     2641 atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat
     2701 cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca
     2761 catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac
     2821 cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa
     2881 taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt
     2941 ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt
     3001 cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac
     3061 cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca
     3121 atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact
     3181 tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat
     3241 ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt
     3301 ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat
     3361 taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt
     3421 tcgacatgat ttatcttcgt ttcctgcagg tttttgttct gtgcagttgg gttaagaata
     3481 ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt
     3541 gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa
     3601 accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaaaaggtgg tatggtgcac
     3661 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     3721 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3781 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     3841 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3901 gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg
     3961 ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata
     4021 aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc
     4081 aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat
     4141 acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac
     4201 acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg
     4261 tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt
     4321 ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta
     4381 aattataatt atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa
     4441 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4501 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4561 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4621 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4681 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4741 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4801 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4861 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4921 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4981 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5041 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5101 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5161 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5221 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5281 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5341 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5401 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5461 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5521 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5581 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5641 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5701 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5761 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5821 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5881 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5941 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6001 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6061 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6121 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa aggccagcaa
     6181 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcgctcgc gtcgatctcc
     6241 cctcaagtgg caaactgcga ctgatatgaa agtaaagtat cggaccttct attacggaat
     6301 gtatacaaac gctacaggat acagtgatat taaatagaaa acaagtctac aattaaatgg
     6361 cagaaagaag aaccagctta ctactaacca ccatactcat caatacccag agcatcggtg
     6421 aacatctgct caaattcgaa gtcagccata tccagcgcac cgtaaggtgc tgaatcatgt
     6481 ggagtgaagc caggccctgg agagtccccg tcacccaaca tatctaaatc aaaatcgtct
     6541 aaagcgtcag cgtgggccat ggcaacgtct tcaccgtcca aatgcagttc gctagatgta
     6601 gaccctgctg tcgctaggtg ggattggtcg gtttcttcaa cggaggctcc gcctctagaa
     6661 gttggtgcat gcaacctgtg agcgtccagc atttctagta ataaatcata cagaggaacg
     6721 acgtttttac acttcataga atacaggtgc tccataccct tgttagacat atgtctaatg
     6781 tgtgacaaaa ttagtagtaa ctgagctagc ctctgatgct gttgctgcag agtcaatcct
     6841 gctttcgcca taaggtgaat caatgtatca gtgatcttgt ccaacactct atgtatatgg
     6901 tctttctctt ccaggctttt cagagttgag cttaaaaatg tatagacacc gctatttaac
     6961 aaaataattg atttcaggca aacgaattct tctccttgca aattcatcat tctgaacctg
     7021 gagctagtgg caagaagcat atcaaaaatc tcaaccatac cttctacgca tttaccttgg
     7081 ttcctgtcca ataataaatt aggtgcaaat aataatttta cagggtgctc catacttctc
     7141 cacactaatc cgatcatcaa tatttctaac cacgcgcatt caagaagatg tacctggtca
     7201 tgtaatgtca agtcaacaaa gccgggaact cttttggccc aattaatcat atgaactaat
     7261 tccctgtctg ctagatttgt caaaagaccc atcatggaag cctcggaaaa aggacgagtt
     7321 gggtcgtatt cactgtacaa tataggtggt tcagcatcaa gtagtgcgga aaccatttga
     7381 tcagcggtca aagacagtgc aagagaattc ttcttagatc ttttgatcat taatgggctt
     7441 ggccacaagt tggctgccct catgtctcca gcgcttggat cttctagggt tgaggctgct
     7501 gccggagtgc ccccacctcc tgtatggatc tttgtatgtc tctttctttc atcagatctg
     7561 gcgaactttc ttccacaaat gtcacacgcg aacggcttct cgccggtgtg ggttctaatg
     7621 tgcgtagtca aatggtcact tctgctgaag tttctcatac agattctaca ttggaaaggc
     7681 ttttggccgg tgtgaatacg aatatgccta gtcagctcgt cagatctgct gaatctccta
     7741 tcacaacttt ctacagggca agcgtagggt ctcgttccca ttgttaattc agtaaatttt
     7801 cgatcttggg aagaaaaaag cagtaagcgt gaaaaatcta aaagctgatg tagtagaaga
     7861 tcctattctt taacaaagat tgaccttttc tttttcttct tggtttgagt agaaagggga
     7921 aggaagaata caagagagag gaaaaaaagg aagataaaaa gagagcgtga tataaatgaa
     7981 tatatattaa acaagagaga ttgggaagga aaggatcaaa caaacccaaa aatatttcaa
     8041 aaaggagaga gagaggcgag tttggtttca aaacggttta tttatttatg caagaggacg
     8101 tggaagaaaa agaagaagga agaaaaaaat ttgaaagaaa aaaacgcgtg gcgggtaaag
     8161 aagaaaatgg aaaatagagg ccgggtgaca gagaaatatt gagggttaat tggaaaatat
     8221 gttagggtga ggcatatgtt tttaaaggga ctagtttata ttgaattttc aaaaattctt
     8281 actttttttt tggatggacg caaagaagtt taataatcat attacatggc attaccacca
     8341 tatacatatc catatacata tccatatcta atcttactta tatgttgtgg aaatgtaaag
     8401 agccccatta tcttagccta aaaaaacctt ctctttggaa ctttcagtaa tacgcttaac
     8461 tgctcattgc tatattgaag tgcggccgcg tgggcgtgcg tgggcgggcg tgggcgtgcg
     8521 tgggcgggcg tgggcgtgcg tgggcgtcta gaccgtgcgt cctcgtcttc accggtcgcg
     8581 ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga acaataaaga ttctacaata
     8641 ctagctttta tggttatgaa gaggaaaaat tggcagtaac ctggccccac aaaccttcaa
     8701 attaacgaat caaattaaca accataggat gataatgcga ttagtttttt agccttattt
     8761 ctggggtaat taatcagcga agcgatgatt tttgatctat taacagatat ataaatggaa
     8821 aagctgcata accactttaa ctaatacttt caacattttc agtttgtatt acttcttatt
     8881 caaatgtcat aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag
     8941 gagaaaaaac tataagggtg cgcatctgat gagtccgtga ggacgaaacg agctagctcg
     9001 tcatgcgcac ccttagcgag aggtttatca ttaaggtcaa cctctggatg ttgtttcggc
     9061 atcctgcatt gaatctgagt tactgtctgt tttcctgggt cacgcgtagg agggttttcc
     9121 attcgtcttg aagtcgagga ctttggcata cgatggaaga gcgtaacttc gttgtaaaga
     9181 ataaggaaat gattccggaa gctttggaag taaggaaacc cgtttcttct gacgtaaggg
     9241 tgcgcaactt tggcatacga tggaaggttt cagagctatg ctggaaacag catagcaagt
     9301 tgaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttgat
     9361 ggccggcatg gtcccagcct cctcgctggc gccggctggg caacaccttc gggtggcgaa
     9421 tgggacttaa agaaagtgga atattcattc atatcatatt ttttctatta actgcctggt
     9481 ttcttttaaa ttttttattg gttgtcgact tgaacggagt gacaatatat atatatatat
     9541 ttaataatga catcattatc tgtaaatctg attcttaatg ctattctagt tatgtaagag
     9601 tggtcctttc cata
//
