LOCUS       sequence-462341-        5280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pOT2
FEATURES             Location/Qualifiers
     source          1..5280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             268..984
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(298..326)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     921..941
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1001..1087
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1777..2439)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2440..3211
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(4493..5026)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5215..5220,5221..5237,5238..5243))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ccatgggcaa atgggatggc tttactaagc tgatccggtg gatgaccttt tgaatgacct
       61 ttaatagatt atattactaa ttaattgggg accctagagg tccccttttt tattttaaaa
      121 attttttcac aaaacggttt acaagcataa agcttgctca atcaatcacc ggatctagat
      181 atcgattaat taagtcgaca tctagagttt aaactttaat taagcccggg ctgcagcccg
      241 ggggatccac tagtggagga agaaaaaatg agtaaaggag aagaactttt cactggagtt
      301 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      361 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      421 aaactacctg ttccatggcc aacacttgtc actactttct cttatggtgt tcaatgcttt
      481 tcccgttatc cggatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
      541 tatgtacagg aacgcactat atctttcaaa gatgacggga actacaagac gcgtgctgaa
      601 gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaaaggtat tgattttaaa
      661 gaagatggaa acattctcgg acacaaactc gagtacaact ataactcaca caatgtatac
      721 atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacatt
      781 gaagatggat ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      841 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
      901 aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat
      961 ggcatggatg agctctacaa ataatgaatt ccccaggcat caaataaaac gaaaggctca
     1021 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     1081 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
     1141 aggacgcccg ccataaactg ccaggaattc caactgagcg ccggtcgcta ccattaccaa
     1201 cttgtctggt gtcaaaaata ataggatttt gttaaaattc gcgttaaatt tttgttaaat
     1261 cagctcattt tttaaccaat aggccgtact gcgatgagtg gcagggcggg gcgtaatttt
     1321 tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata
     1381 agcggatgaa tggcagaaat tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg
     1441 tcgttaaata gccgcttatg tctattgctg gtttaccggt ttattgacta ccggaagcag
     1501 tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct caggctctcc ccgtggaggt
     1561 aataattgac gatatgatca tttattctgc ctcccagagc ctgataaaaa cggtgaatcc
     1621 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
     1681 cgcaacgcgg ggaggcagac aaggtatagg gcggcgaggc ggctacagcc gatagtctgg
     1741 aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg
     1801 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag
     1861 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc
     1921 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg
     1981 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc
     2041 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg
     2101 gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt
     2161 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt
     2221 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg
     2281 gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat
     2341 aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt
     2401 cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg
     2461 gtgccgcacg gttgcggcac catgcgcaat cagctgcaac ttttcggcag cgcgacaaca
     2521 attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta cgcaatgagc
     2581 tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt tgttgatttt
     2641 taagtctttc gcatttcgcc ctatatctag ttctttggtg cccaaagaag ggcacccctg
     2701 cggggttccc ccacgccttc ggcgcggctc cccctccggc aaaaagtggc ccctccgggg
     2761 cttgttgatc gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc ccttggaacc
     2821 cccgcactcg ccgccgtgag gctcgggggg caggcgggcg ggcttcgccc ttcgactgcc
     2881 cccactcgca taggcttggg tcgttccagg cgcgtcaagg ccaagccgct gcgcggtcgc
     2941 tgcgcgagcc ttgacccgcc ttccacttgg tgtccaaccg gcaagcgaag cgcgcaggcc
     3001 gcaggccgga ggcttttccc cagagaaaat taaaaaaatt gatggggcaa ggccgcaggc
     3061 cgcgcagttg gagccggtgg gtatgtggtc gaaggctggg tagccggtgg gcaatccctg
     3121 tggtcaagct cgtgggcagg cgcagcctgt ccatcagctt gtccagcagg gttgtccacg
     3181 ggccgagcga agcgagccag ccggtggccg ctcgcggcca tcgtccacat atccacgggc
     3241 tggcaaggga gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc gtagggcgcc
     3301 gcagccgccg taggcggtca cgactttgcg aagcaaagtc tagtgagtat actcaagcat
     3361 tgagtggccc gccggaggca ccgccttgcg ctgcccccgt cgagccggtt ggacaccaaa
     3421 agggaggggc aggcatggcg gcatacgcga tcatgcgatg caagaagctg gcgaaaatgg
     3481 gcaacgtggc ggccagtctc aagcacgcct accgcgagcg cgagacgccc aacgctgacg
     3541 ccagcaggac gccagagaac gagcactggg cggccagcag caccgatgaa gcgatgggcc
     3601 gactgcgcga gttgctgcca gagaagcggc gcaaggacgc tgtgttggcg gtcgagtacg
     3661 tcatgacggc cagcccggaa tggtggaagt cggccagcca agaacagcag gcggcgttct
     3721 tcgagaaggc gcacaagtgg ctggcggaca agtacggggc ggatcgcatc gtgacggcca
     3781 gcatccaccg tgacgaaacc agcccgcaca tgaccgcgtt cgtggtgccg ctgacgcagg
     3841 acggcaggct gtcggccaag gagttcatcg gcaacaaagc gcagatgacc cgcgaccaga
     3901 ccacgtttgc ggccgctgtg gccgatctag ggctgcaacg gggcatcgag ggcagcaagg
     3961 cacgtcacac gcgcattcag gcgttctacg aggccctgga gcggccacca gtgggccacg
     4021 tcaccatcag cccgcaagcg gtcgagccac gcgcctatgc accgcaggga ttggccgaaa
     4081 agctgggaat ctcaaagcgc gttgagacgc cggaagccgt ggccgaccgg ctgacaaaag
     4141 cggttcggca ggggtatgag cctgccctac aggccgccgc aggagcgcgt gagatgcgca
     4201 agaaggccga tcaagcccaa gagacggccc gagaccttcg ggagcgcctg aagcccgttc
     4261 tggacgccct ggggccgttg aatcgggata tgcaggccaa ggccgccgcg atcatcaagg
     4321 ccgtgggcga aaagctgctg acggaacagc gggaagtcca gcgccagaaa caggcccagc
     4381 gccagcagga acgcgggcgc gcacatttcc ccgaaaagtg ccacctgcgg cgttgtgaca
     4441 atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg
     4501 cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga
     4561 gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg
     4621 cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct
     4681 ctccggagac tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca
     4741 gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga
     4801 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
     4861 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
     4921 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
     4981 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat
     5041 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     5101 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     5161 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     5221 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
//
