LOCUS       sequence-462342-        5391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1101
FEATURES             Location/Qualifiers
     source          1..5391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             324..1037
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             324..1037
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(354..382)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     977..997
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1112..1198
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1888..2550)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2551..3322
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(4604..5137)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5326..5331,5332..5348,5349..5354))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ccatgggcaa atgggatggc tttactaagc tgatccggtg gatgaccttt tgaatgacct
       61 ttaatagatt atattactaa ttaattgggg accctagagg tccccttttt tattttaaaa
      121 attttttcac aaaacggttt acaagcataa agcttgctca atcaatcacc ggatctagat
      181 atcgattaat taagtcgaca tctagagttt aaactttaat taagcccggg ctgcagcccg
      241 ggggatccac taggttaacg catgcggccc ggtaccaggc ctagatagat agagagagag
      301 agagactagt ggaggaagaa aaaatgcgta aaggagaaga acttttcact ggagttgtcc
      361 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      421 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      481 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      541 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      601 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      661 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      721 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      781 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      841 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      901 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      961 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     1021 tggatgaact atacaaaagg cctgcagcaa acgacgaaaa ctacgctgca tcagtttaat
     1081 gagctctaca aataatgaat tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1141 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     1201 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     1261 gccataaact gccaggaatt ccaactgagc gccggtcgct accattacca acttgtctgg
     1321 tgtcaaaaat aataggattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     1381 ttttaaccaa taggccgtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
     1441 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga
     1501 atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt tcagggcagg gtcgttaaat
     1561 agccgcttat gtctattgct ggtttaccgg tttattgact accggaagca gtgtgaccgt
     1621 gtgcttctca aatgcctgag gccagtttgc tcaggctctc cccgtggagg taataattga
     1681 cgatatgatc atttattctg cctcccagag cctgataaaa acggtgaatc cgttagcgag
     1741 gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg
     1801 gggaggcaga caaggtatag ggcggcgagg cggctacagc cgatagtctg gaacagcgca
     1861 cttacgggtt gctgcgcaac ccaagtgcta ccggcgcggc agcgtgaccc gtgtcggcgg
     1921 ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg ggcatcggca ggcgctgctg
     1981 cccgcgccgt tcccattcct ccgtttcggt caaggctggc aggtctggtt ccatgcccgg
     2041 aatgccgggc tggctgggcg gctcctcgcc ggggccggtc ggtagttgct gctcgcccgg
     2101 atacagggtc gggatgcggc gcaggtcgcc atgccccaac agcgattcgt cctggtcgtc
     2161 gtgatcaacc accacggcgg cactgaacac cgacaggcgc aactggtcgc ggggctggcc
     2221 ccacgccacg cggtcattga ccacgtaggc cgacacggtg ccggggccgt tgagcttcac
     2281 gacggagatc cagcgctcgg ccaccaagtc cttgactgcg tattggaccg tccgcaaaga
     2341 acgtccgatg agcttggaaa gtgtcttctg gctgaccacc acggcgttct ggtggcccat
     2401 ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt ttcctcgcaa taagcccggc
     2461 ccacgcctca tgcgctttgc gttccgtttg cacccagtga ccgggcttgt tcttggcttg
     2521 aatgccgatt tctctggact gcgtggccat gcttatctcc atgcggtagg ggtgccgcac
     2581 ggttgcggca ccatgcgcaa tcagctgcaa cttttcggca gcgcgacaac aattatgcgt
     2641 tgcgtaaaag tggcagtcaa ttacagattt tctttaacct acgcaatgag ctattgcggg
     2701 gggtgccgca atgagctgtt gcgtaccccc cttttttaag ttgttgattt ttaagtcttt
     2761 cgcatttcgc cctatatcta gttctttggt gcccaaagaa gggcacccct gcggggttcc
     2821 cccacgcctt cggcgcggct ccccctccgg caaaaagtgg cccctccggg gcttgttgat
     2881 cgactgcgcg gccttcggcc ttgcccaagg tggcgctgcc cccttggaac ccccgcactc
     2941 gccgccgtga ggctcggggg gcaggcgggc gggcttcgcc cttcgactgc ccccactcgc
     3001 ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc
     3061 cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg
     3121 aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt
     3181 ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc
     3241 tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac gggccgagcg
     3301 aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg ctggcaaggg
     3361 agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc cgcagccgcc
     3421 gtaggcggtc acgactttgc gaagcaaagt ctagtgagta tactcaagca ttgagtggcc
     3481 cgccggaggc accgccttgc gctgcccccg tcgagccggt tggacaccaa aagggagggg
     3541 caggcatggc ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg ggcaacgtgg
     3601 cggccagtct caagcacgcc taccgcgagc gcgagacgcc caacgctgac gccagcagga
     3661 cgccagagaa cgagcactgg gcggccagca gcaccgatga agcgatgggc cgactgcgcg
     3721 agttgctgcc agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac gtcatgacgg
     3781 ccagcccgga atggtggaag tcggccagcc aagaacagca ggcggcgttc ttcgagaagg
     3841 cgcacaagtg gctggcggac aagtacgggg cggatcgcat cgtgacggcc agcatccacc
     3901 gtgacgaaac cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag gacggcaggc
     3961 tgtcggccaa ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag accacgtttg
     4021 cggccgctgt ggccgatcta gggctgcaac ggggcatcga gggcagcaag gcacgtcaca
     4081 cgcgcattca ggcgttctac gaggccctgg agcggccacc agtgggccac gtcaccatca
     4141 gcccgcaagc ggtcgagcca cgcgcctatg caccgcaggg attggccgaa aagctgggaa
     4201 tctcaaagcg cgttgagacg ccggaagccg tggccgaccg gctgacaaaa gcggttcggc
     4261 aggggtatga gcctgcccta caggccgccg caggagcgcg tgagatgcgc aagaaggccg
     4321 atcaagccca agagacggcc cgagaccttc gggagcgcct gaagcccgtt ctggacgccc
     4381 tggggccgtt gaatcgggat atgcaggcca aggccgccgc gatcatcaag gccgtgggcg
     4441 aaaagctgct gacggaacag cgggaagtcc agcgccagaa acaggcccag cgccagcagg
     4501 aacgcgggcg cgcacatttc cccgaaaagt gccacctgcg gcgttgtgac aatttaccga
     4561 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     4621 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     4681 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     4741 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tctccggaga
     4801 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
     4861 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     4921 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     4981 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     5041 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     5101 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5161 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     5221 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     5281 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     5341 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg t
//
